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The map label for this gene is ydiB [C]

Identifier: 182680196

GI number: 182680196

Start: 3746475

End: 3747293

Strand: Reverse

Name: ydiB [C]

Synonym: Bind_3294

Alternate gene names: 182680196

Gene position: 3747293-3746475 (Counterclockwise)

Preceding gene: 182680197

Following gene: 182680195

Centisome position: 89.86

GC content: 54.58

Gene sequence:

>819_bases
ATGCCGAGAACCATCAACAAAGACACGATGTTATGTATGTCCCTTTCGGGGCGACCAGGAAATTTCGGAACGCGCTTTCA
TAATTTTCTTTATGAAGAGCTCGATCTCAATTTCATCTACAAGGCCTTTACCACGCAGGATTTGCGCGCGGCGATCGGCG
GGATCAGGGCGCTGGGCATTCGAGGCTGCGCCGTTTCCATGCCTTTCAAGGAAGCCTGTATCGAATTTCTGGATGAACTG
GATGCATCCGCCACAGCGATCCAATCGGTCAATACGATCGTCAATGATAAAGGCTGGCTGCGTGCTTATAATACCGACTA
TATTGCCGTCACCAGCCTCTTGAAATCCCATGAGGTGCCGCCCCAATCCGTGTTCGTTGTGCGCGGCAGCGGCGGAATGG
CAAAGGCCGTTCTCTGTGCTCTTCGTGATTCAGGGTTTCGCTCTGGCTTTGTTCTCGCCAGGAATGAAACAGCTGGCCGG
GCACTGGCCCAAATCTATGATTATCAATGGATCAAGACATTGGACGGCATAAAGGCGGATATGCTCGTCAATGTCACGCC
GATTGGCATGGCCGGCGGTCCGGAAGCCGATGATCTCGCTTTTGAGCCCGACGCCATCGAGAAAGCCAGCACGATTTTCG
ATGTGGTTGCGCTTCCCGTTGAGACCCCTTTGATCCGCCATGCCAGTGCCAAAGGGAAAAGTGTGATCACCGGCGCGGAC
GTGATCGCGCTGCAGGCCGTGGAACAATTCGTCCTTTATACCGGTTTGCGGCCGAACGACGACCAGATCCGCCGTGCCGC
GGATTTCGCGCGCACTTGA

Upstream 100 bases:

>100_bases
AAATCGCGATCATGCCAGGCACGACCGCACGCCATCAAAAGGGCCGTCCCTGATCATCATGCCGTGCGAACGGAAGCATA
CACTATCTTGAGGATTCTTC

Downstream 100 bases:

>100_bases
GTACGGTTGGGGCTATGCCTTCGCGGCAGGATCGAAACGCTTGACAAGCTTTTTTTGTCCTGTATTTTTGCATAATACCT
ATACATAAATAGCTGAGCTC

Product: shikimate dehydrogenase substrate binding subunit

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MPRTINKDTMLCMSLSGRPGNFGTRFHNFLYEELDLNFIYKAFTTQDLRAAIGGIRALGIRGCAVSMPFKEACIEFLDEL
DASATAIQSVNTIVNDKGWLRAYNTDYIAVTSLLKSHEVPPQSVFVVRGSGGMAKAVLCALRDSGFRSGFVLARNETAGR
ALAQIYDYQWIKTLDGIKADMLVNVTPIGMAGGPEADDLAFEPDAIEKASTIFDVVALPVETPLIRHASAKGKSVITGAD
VIALQAVEQFVLYTGLRPNDDQIRRAADFART

Sequences:

>Translated_272_residues
MPRTINKDTMLCMSLSGRPGNFGTRFHNFLYEELDLNFIYKAFTTQDLRAAIGGIRALGIRGCAVSMPFKEACIEFLDEL
DASATAIQSVNTIVNDKGWLRAYNTDYIAVTSLLKSHEVPPQSVFVVRGSGGMAKAVLCALRDSGFRSGFVLARNETAGR
ALAQIYDYQWIKTLDGIKADMLVNVTPIGMAGGPEADDLAFEPDAIEKASTIFDVVALPVETPLIRHASAKGKSVITGAD
VIALQAVEQFVLYTGLRPNDDQIRRAADFART
>Mature_271_residues
PRTINKDTMLCMSLSGRPGNFGTRFHNFLYEELDLNFIYKAFTTQDLRAAIGGIRALGIRGCAVSMPFKEACIEFLDELD
ASATAIQSVNTIVNDKGWLRAYNTDYIAVTSLLKSHEVPPQSVFVVRGSGGMAKAVLCALRDSGFRSGFVLARNETAGRA
LAQIYDYQWIKTLDGIKADMLVNVTPIGMAGGPEADDLAFEPDAIEKASTIFDVVALPVETPLIRHASAKGKSVITGADV
IALQAVEQFVLYTGLRPNDDQIRRAADFART

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=253, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320332, Length=257, Percent_Identity=26.4591439688716, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 29602; Mature: 29471

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTINKDTMLCMSLSGRPGNFGTRFHNFLYEELDLNFIYKAFTTQDLRAAIGGIRALGI
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
RGCAVSMPFKEACIEFLDELDASATAIQSVNTIVNDKGWLRAYNTDYIAVTSLLKSHEVP
CCEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCC
PQSVFVVRGSGGMAKAVLCALRDSGFRSGFVLARNETAGRALAQIYDYQWIKTLDGIKAD
CCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
MLVNVTPIGMAGGPEADDLAFEPDAIEKASTIFDVVALPVETPLIRHASAKGKSVITGAD
EEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEECCHH
VIALQAVEQFVLYTGLRPNDDQIRRAADFART
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PRTINKDTMLCMSLSGRPGNFGTRFHNFLYEELDLNFIYKAFTTQDLRAAIGGIRALGI
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
RGCAVSMPFKEACIEFLDELDASATAIQSVNTIVNDKGWLRAYNTDYIAVTSLLKSHEVP
CCEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCC
PQSVFVVRGSGGMAKAVLCALRDSGFRSGFVLARNETAGRALAQIYDYQWIKTLDGIKAD
CCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCC
MLVNVTPIGMAGGPEADDLAFEPDAIEKASTIFDVVALPVETPLIRHASAKGKSVITGAD
EEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEECCHH
VIALQAVEQFVLYTGLRPNDDQIRRAADFART
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]