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The map label for this gene is tgt [H]

Identifier: 182679291

GI number: 182679291

Start: 2672362

End: 2673522

Strand: Reverse

Name: tgt [H]

Synonym: Bind_2335

Alternate gene names: 182679291

Gene position: 2673522-2672362 (Counterclockwise)

Preceding gene: 182679292

Following gene: 182679290

Centisome position: 64.11

GC content: 60.38

Gene sequence:

>1161_bases
ATGAGTGAACCGGCTGAATCCGAAACGGCTTTTCGCTTCAAGCTCCTGCATCAGGACGGAGCGGCGCGTGCCGGTGTCAT
CTCGACGCCGCGTGGAGAGATCCGCACGCCGGCTTTCATGCCGGTGGGCACGGCGGCAACGGTGAAAGCCATGCAGCCCG
AGGCGGTGAGGGCGCTAGGCGCCGATATTGTTCTCTCTAACACCTATCATCTGATGTTGCGCCCCGGTGCCGAGCGGATT
GCGGCCTTGGGCGGCCTGCATCGCTTCATGAACTGGCCACATCCCATTCTGACCGATTCCGGCGGCTTTCAGGTGATGTC
GCTCGCGAAATTGCGCAAACTCGATGAAAACGGCGTCACTTTTCAATCGCATATCGATGGCGCCCGGCATGTTTTGACGC
CTGAGCGCTCGATGGAGATCCAGGATCTCCTCGGCTCGGATATTGTGATGCAATTCGATGAATGTGTGCGGCTTCCTTGC
GCGCCGGGAGAGGCCGAACGGGCCATGCGGCTGTCCTTGCGCTGGGGTGAGCGCAGCAAGCGGGCCTTGGCGCCCAAATC
CGGACGCGCGCTTTTCGGCATTGTGCAAGGTGGCGATGACAAGGCGCTCAGACGGGAAAGTGCCACGGAACTCGTGAGCA
TGGGGTTCGACGGTTATGCCTTGGGTGGTCTCGCGGTCGGCGAGCCGCAAGCGGTCATGCTCGACATGATCGAGACGGTC
ATGCCTTTCTTGCCGGTTGATAAGCCTCATTATCTGATGGGCGTCGGCACACCGGACGATCTTCTGCAAAGCGTCAAACG
CGGCATCGATATGTTCGATTGTGTCATGCCAACGCGTGCCGGGCGGCATGGCCTGGTGTTTACCCGCCATGGCCGGATCA
ACCTGCGCAATGCCCGCCATGCCGATGATCCTGCGCCGATCGATGAGAAATCGTCCTGTGCCGCTGCCAGGATTTATTCA
CGCGCCTATCTGCATCATTTGGTGAAGGCCAGTGAGATTCTCGGCATGATCCTCATGACGACGATCAATCTCGCCTATTA
TCAGGAGCTGATGGCGGGGATGCGGGCGGCGATCGCCGAGGGCCGGTTTGAGGACTTCGCGGCAGAGACCGAGGCTCTCT
GGCGTGAAGGCGAAAGCCTGAGCGGGCGGGCAAGCGCATAA

Upstream 100 bases:

>100_bases
CCTATGCCCATGCGATCGCGGCCTCCTATCGGTTCTACAGCTATGGTGATGCCTGTCTGTTGTTTCGCGCGACAAGGAAT
ACATCGACGGGGGCCGCGTT

Downstream 100 bases:

>100_bases
GATCGGATTTAAAGATTGGAAAATGACCAATGAAAAAGAGGGGCGGGATCTTCCCAGGATCCCTGCCCCTCTTTATCTAG
CCTCAGTCCTTGAACCTACC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSEPAESETAFRFKLLHQDGAARAGVISTPRGEIRTPAFMPVGTAATVKAMQPEAVRALGADIVLSNTYHLMLRPGAERI
AALGGLHRFMNWPHPILTDSGGFQVMSLAKLRKLDENGVTFQSHIDGARHVLTPERSMEIQDLLGSDIVMQFDECVRLPC
APGEAERAMRLSLRWGERSKRALAPKSGRALFGIVQGGDDKALRRESATELVSMGFDGYALGGLAVGEPQAVMLDMIETV
MPFLPVDKPHYLMGVGTPDDLLQSVKRGIDMFDCVMPTRAGRHGLVFTRHGRINLRNARHADDPAPIDEKSSCAAARIYS
RAYLHHLVKASEILGMILMTTINLAYYQELMAGMRAAIAEGRFEDFAAETEALWREGESLSGRASA

Sequences:

>Translated_386_residues
MSEPAESETAFRFKLLHQDGAARAGVISTPRGEIRTPAFMPVGTAATVKAMQPEAVRALGADIVLSNTYHLMLRPGAERI
AALGGLHRFMNWPHPILTDSGGFQVMSLAKLRKLDENGVTFQSHIDGARHVLTPERSMEIQDLLGSDIVMQFDECVRLPC
APGEAERAMRLSLRWGERSKRALAPKSGRALFGIVQGGDDKALRRESATELVSMGFDGYALGGLAVGEPQAVMLDMIETV
MPFLPVDKPHYLMGVGTPDDLLQSVKRGIDMFDCVMPTRAGRHGLVFTRHGRINLRNARHADDPAPIDEKSSCAAARIYS
RAYLHHLVKASEILGMILMTTINLAYYQELMAGMRAAIAEGRFEDFAAETEALWREGESLSGRASA
>Mature_385_residues
SEPAESETAFRFKLLHQDGAARAGVISTPRGEIRTPAFMPVGTAATVKAMQPEAVRALGADIVLSNTYHLMLRPGAERIA
ALGGLHRFMNWPHPILTDSGGFQVMSLAKLRKLDENGVTFQSHIDGARHVLTPERSMEIQDLLGSDIVMQFDECVRLPCA
PGEAERAMRLSLRWGERSKRALAPKSGRALFGIVQGGDDKALRRESATELVSMGFDGYALGGLAVGEPQAVMLDMIETVM
PFLPVDKPHYLMGVGTPDDLLQSVKRGIDMFDCVMPTRAGRHGLVFTRHGRINLRNARHADDPAPIDEKSSCAAARIYSR
AYLHHLVKASEILGMILMTTINLAYYQELMAGMRAAIAEGRFEDFAAETEALWREGESLSGRASA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=345, Percent_Identity=44.9275362318841, Blast_Score=298, Evalue=6e-81,
Organism=Escherichia coli, GI1786607, Length=370, Percent_Identity=53.7837837837838, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17544680, Length=359, Percent_Identity=41.7827298050139, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI19920510, Length=348, Percent_Identity=44.2528735632184, Blast_Score=307, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42212; Mature: 42081

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPAESETAFRFKLLHQDGAARAGVISTPRGEIRTPAFMPVGTAATVKAMQPEAVRALG
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHC
ADIVLSNTYHLMLRPGAERIAALGGLHRFMNWPHPILTDSGGFQVMSLAKLRKLDENGVT
CCEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCCCE
FQSHIDGARHVLTPERSMEIQDLLGSDIVMQFDECVRLPCAPGEAERAMRLSLRWGERSK
EHHHCCCCCEECCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHEEEEEECCCCCC
RALAPKSGRALFGIVQGGDDKALRRESATELVSMGFDGYALGGLAVGEPQAVMLDMIETV
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHH
MPFLPVDKPHYLMGVGTPDDLLQSVKRGIDMFDCVMPTRAGRHGLVFTRHGRINLRNARH
HHHCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCCEEECCCCC
ADDPAPIDEKSSCAAARIYSRAYLHHLVKASEILGMILMTTINLAYYQELMAGMRAAIAE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRFEDFAAETEALWREGESLSGRASA
CCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEPAESETAFRFKLLHQDGAARAGVISTPRGEIRTPAFMPVGTAATVKAMQPEAVRALG
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHC
ADIVLSNTYHLMLRPGAERIAALGGLHRFMNWPHPILTDSGGFQVMSLAKLRKLDENGVT
CCEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCCCE
FQSHIDGARHVLTPERSMEIQDLLGSDIVMQFDECVRLPCAPGEAERAMRLSLRWGERSK
EHHHCCCCCEECCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHEEEEEECCCCCC
RALAPKSGRALFGIVQGGDDKALRRESATELVSMGFDGYALGGLAVGEPQAVMLDMIETV
CCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEECCEECCCCHHHHHHHHHHH
MPFLPVDKPHYLMGVGTPDDLLQSVKRGIDMFDCVMPTRAGRHGLVFTRHGRINLRNARH
HHHCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCCEEECCCCC
ADDPAPIDEKSSCAAARIYSRAYLHHLVKASEILGMILMTTINLAYYQELMAGMRAAIAE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRFEDFAAETEALWREGESLSGRASA
CCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]