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The map label for this gene is queA

Identifier: 182679292

GI number: 182679292

Start: 2673519

End: 2674622

Strand: Reverse

Name: queA

Synonym: Bind_2336

Alternate gene names: 182679292

Gene position: 2674622-2673519 (Counterclockwise)

Preceding gene: 182679293

Following gene: 182679291

Centisome position: 64.14

GC content: 59.78

Gene sequence:

>1104_bases
ATGCGCGTCGATCTTTTCGATTTCGATCTGCCACCGGAGCGCATCGCCTTGCGCCCGGTCGAGCCACGTGATGCTTCACG
GCTTCTCGTAGTGCGTCCCGACAGCGGGGACATGACCGATCATGGGATGCGGGAATTGCCGGATTTCCTGCGTGCAGGCG
ATGTTCTCGTCGTCAATGACACGCGGGTCATTCCGGCGCGCCTCCATGGATTTCGTTCACGCGGGGAGAGCCGGGCGAAA
ATCGAGGCCACCTTGCACAAGCGCGAGGGGGAAGCCCTTTGGCGAGCCTTCGTGAAACCGGCTAAAAAACTTCGGGTCGG
TGAAACAATTTGCTTCGCGCGCCAAGAGCCTGGGACAGAGGTTGAAAGCCTTGAGGCCGAGGTTTTGGAAAAAGGTGCTG
AAGGCGAGGTCGTTCTCGGCTTCAACCGCGCAGGCGCGGCACTCGATGCGGCGCTTGATCTTCTCGGCGAAATGCCGCTG
CCGCCCTATATTGCTGGCAAAAGGGCGCCCGACAGCCAGGATTCCTTGGATTATCAGACACTCTTTGCCAACCGGTCCGG
CGCGGTCGCGGCACCGACCGCGAGTCTGCATTTCACGCCCCGGCTGATCGCGGCCATAGAGGCGAGGGGTGTCACGATTT
GCAAAGTGACATTGCATGTCGGGGCAGGGACCTTTCTGCCGGTCAAAGCCGAAGATACAGACGCGCACCGCATGCATGCC
GAATGGGGGGAAGTCAGTGCCGAGGTCGCGGCTTTCTTGAATAAGGTCCATGCCGCCGGTGGACGGATTATCGCCGCCGG
CACGACAAGCCTGCGTCTGCTCGAATCAGCCGTGGACGAAGACGGCCTTATTCAGCCCTTTCAGGGCGAAACCTCGATCT
TCATGACGCCGGGTTTCAGGTTTCGCGCCGTGGATATTCTCTTGACCAATTTTCATCTGCCGAGATCGACGCTCTTCATG
CTCGTCTGCGCCTTTGCCGGACTCGAAACCATGCGGCGGGCCTATGCCCATGCGATCGCGGCCTCCTATCGGTTCTACAG
CTATGGTGATGCCTGTCTGTTGTTTCGCGCGACAAGGAATACATCGACGGGGGCCGCGTTATGA

Upstream 100 bases:

>100_bases
GCCGGTGCGCGATCCCGACAAAATTATTTCCGCGACCCTGAGCTAATCACAAAGGCTGTTCTCGTGCGGTTCGCCCGAGA
AGGGCGGATCGCCATCCACC

Downstream 100 bases:

>100_bases
GTGAACCGGCTGAATCCGAAACGGCTTTTCGCTTCAAGCTCCTGCATCAGGACGGAGCGGCGCGTGCCGGTGTCATCTCG
ACGCCGCGTGGAGAGATCCG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRVDLFDFDLPPERIALRPVEPRDASRLLVVRPDSGDMTDHGMRELPDFLRAGDVLVVNDTRVIPARLHGFRSRGESRAK
IEATLHKREGEALWRAFVKPAKKLRVGETICFARQEPGTEVESLEAEVLEKGAEGEVVLGFNRAGAALDAALDLLGEMPL
PPYIAGKRAPDSQDSLDYQTLFANRSGAVAAPTASLHFTPRLIAAIEARGVTICKVTLHVGAGTFLPVKAEDTDAHRMHA
EWGEVSAEVAAFLNKVHAAGGRIIAAGTTSLRLLESAVDEDGLIQPFQGETSIFMTPGFRFRAVDILLTNFHLPRSTLFM
LVCAFAGLETMRRAYAHAIAASYRFYSYGDACLLFRATRNTSTGAAL

Sequences:

>Translated_367_residues
MRVDLFDFDLPPERIALRPVEPRDASRLLVVRPDSGDMTDHGMRELPDFLRAGDVLVVNDTRVIPARLHGFRSRGESRAK
IEATLHKREGEALWRAFVKPAKKLRVGETICFARQEPGTEVESLEAEVLEKGAEGEVVLGFNRAGAALDAALDLLGEMPL
PPYIAGKRAPDSQDSLDYQTLFANRSGAVAAPTASLHFTPRLIAAIEARGVTICKVTLHVGAGTFLPVKAEDTDAHRMHA
EWGEVSAEVAAFLNKVHAAGGRIIAAGTTSLRLLESAVDEDGLIQPFQGETSIFMTPGFRFRAVDILLTNFHLPRSTLFM
LVCAFAGLETMRRAYAHAIAASYRFYSYGDACLLFRATRNTSTGAAL
>Mature_367_residues
MRVDLFDFDLPPERIALRPVEPRDASRLLVVRPDSGDMTDHGMRELPDFLRAGDVLVVNDTRVIPARLHGFRSRGESRAK
IEATLHKREGEALWRAFVKPAKKLRVGETICFARQEPGTEVESLEAEVLEKGAEGEVVLGFNRAGAALDAALDLLGEMPL
PPYIAGKRAPDSQDSLDYQTLFANRSGAVAAPTASLHFTPRLIAAIEARGVTICKVTLHVGAGTFLPVKAEDTDAHRMHA
EWGEVSAEVAAFLNKVHAAGGRIIAAGTTSLRLLESAVDEDGLIQPFQGETSIFMTPGFRFRAVDILLTNFHLPRSTLFM
LVCAFAGLETMRRAYAHAIAASYRFYSYGDACLLFRATRNTSTGAAL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=357, Percent_Identity=42.8571428571429, Blast_Score=252, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_BEII9 (B2IHG4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833438.1
- GeneID:   6201054
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_2336
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40043; Mature: 40043

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFDLPPERIALRPVEPRDASRLLVVRPDSGDMTDHGMRELPDFLRAGDVLVVND
CEEEEECCCCCHHHEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEC
TRVIPARLHGFRSRGESRAKIEATLHKREGEALWRAFVKPAKKLRVGETICFARQEPGTE
CEEEHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
VESLEAEVLEKGAEGEVVLGFNRAGAALDAALDLLGEMPLPPYIAGKRAPDSQDSLDYQT
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LFANRSGAVAAPTASLHFTPRLIAAIEARGVTICKVTLHVGAGTFLPVKAEDTDAHRMHA
EEECCCCCEECCCCCEECCHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCCCHHHHHH
EWGEVSAEVAAFLNKVHAAGGRIIAAGTTSLRLLESAVDEDGLIQPFQGETSIFMTPGFR
HHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
FRAVDILLTNFHLPRSTLFMLVCAFAGLETMRRAYAHAIAASYRFYSYGDACLLFRATRN
EEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCEEEEEEECCC
TSTGAAL
CCCCCCC
>Mature Secondary Structure
MRVDLFDFDLPPERIALRPVEPRDASRLLVVRPDSGDMTDHGMRELPDFLRAGDVLVVND
CEEEEECCCCCHHHEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEC
TRVIPARLHGFRSRGESRAKIEATLHKREGEALWRAFVKPAKKLRVGETICFARQEPGTE
CEEEHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
VESLEAEVLEKGAEGEVVLGFNRAGAALDAALDLLGEMPLPPYIAGKRAPDSQDSLDYQT
HHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LFANRSGAVAAPTASLHFTPRLIAAIEARGVTICKVTLHVGAGTFLPVKAEDTDAHRMHA
EEECCCCCEECCCCCEECCHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCCCHHHHHH
EWGEVSAEVAAFLNKVHAAGGRIIAAGTTSLRLLESAVDEDGLIQPFQGETSIFMTPGFR
HHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
FRAVDILLTNFHLPRSTLFMLVCAFAGLETMRRAYAHAIAASYRFYSYGDACLLFRATRN
EEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCEEEEEEECCC
TSTGAAL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA