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The map label for this gene is nocT [H]

Identifier: 182678758

GI number: 182678758

Start: 2026010

End: 2026855

Strand: Direct

Name: nocT [H]

Synonym: Bind_1787

Alternate gene names: 182678758

Gene position: 2026010-2026855 (Clockwise)

Preceding gene: 182678756

Following gene: 182678759

Centisome position: 48.58

GC content: 53.66

Gene sequence:

>846_bases
ATGCAGATAGATTTTCCCTTCTCGCTTCATACCTTGATCCGCCGTCACCAGGAGGGCCTGGGAACCGGGGTTCTCACCCG
TTTCCTCGTTTATTCTTTCCTCGCCATTGTCCTCATGTCGTCGCCCTGCCGCGCTACGGACACCCCTGGTCCGGAATTGA
GGATCGCCAGCGAAGGCGCGCGGCCGCCTTATAATTATCTCGAAAATAACGAATTAGCCGGTTTCGAGATCGATCTTGGC
CAGGAACTCTGCAAACGCATGAACAGGGTTTGCCGTTTCGTTCCGCAGGATTGGGATAATCTGATTCCTGGTCTTCTCGA
TCATCGCTATGACGCAATCATGGCGGCCTTGGACATCAGTGACGCCGCCAAGGAAAAGATTGCCTTCAGCGAGCCCTATG
TGCGCATGCCCATGGCTCTCCTCGGCAATCGTGAAGAGAAACTTCCTGACACGAGCCCCAAGGGCCTCACTGGCAAGAAA
CTCGGCGTCGAAGCAAACAGCCTCTATCAAGCCTATGCCGAAGATCATTTCGACCATTCGCAAATTCAGACCTATGCCAG
CCTGGAAGAAGCCATCCTAGATCTGGGCGAGGGCCGCATCGACCTCGTCCTCGGCAACAAAGCCGATATCAGCGATTTCC
TGAAAAACCGGCGCGAGGCGCAAAATACCGCCATTTTGGGAGAACTGCCTCATGATGCCGCTTATCTTGGCGAAGGCATC
GGCATCGGCCTGCGTAAAGAGGATAAGGAACTCAAGCGGCAGTTCGATCAAGCGCTCGAGGACATCAACAAGGACGGTAC
ATTTACCAAGATACGCGAACGTTATTTCCCTTATGCGATCAATTGA

Upstream 100 bases:

>100_bases
TCGATTGTCCGCCCAAGGATCATATGATGGCTGCACAAGAAAACCGATCGATCCTGCCATCCGGCCCAAAACTGCGCGGC
CCGAGAGCATGCTACCCAAG

Downstream 100 bases:

>100_bases
ACCATTGGGAAAGTATCAGCGGGGGAGCGCTTCAGCTCGATCACGCTCCCTAAGAACAATTCAAATGCGCGCGATACCGA
TACGTCGAAGCGCATGAACA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MQIDFPFSLHTLIRRHQEGLGTGVLTRFLVYSFLAIVLMSSPCRATDTPGPELRIASEGARPPYNYLENNELAGFEIDLG
QELCKRMNRVCRFVPQDWDNLIPGLLDHRYDAIMAALDISDAAKEKIAFSEPYVRMPMALLGNREEKLPDTSPKGLTGKK
LGVEANSLYQAYAEDHFDHSQIQTYASLEEAILDLGEGRIDLVLGNKADISDFLKNRREAQNTAILGELPHDAAYLGEGI
GIGLRKEDKELKRQFDQALEDINKDGTFTKIRERYFPYAIN

Sequences:

>Translated_281_residues
MQIDFPFSLHTLIRRHQEGLGTGVLTRFLVYSFLAIVLMSSPCRATDTPGPELRIASEGARPPYNYLENNELAGFEIDLG
QELCKRMNRVCRFVPQDWDNLIPGLLDHRYDAIMAALDISDAAKEKIAFSEPYVRMPMALLGNREEKLPDTSPKGLTGKK
LGVEANSLYQAYAEDHFDHSQIQTYASLEEAILDLGEGRIDLVLGNKADISDFLKNRREAQNTAILGELPHDAAYLGEGI
GIGLRKEDKELKRQFDQALEDINKDGTFTKIRERYFPYAIN
>Mature_281_residues
MQIDFPFSLHTLIRRHQEGLGTGVLTRFLVYSFLAIVLMSSPCRATDTPGPELRIASEGARPPYNYLENNELAGFEIDLG
QELCKRMNRVCRFVPQDWDNLIPGLLDHRYDAIMAALDISDAAKEKIAFSEPYVRMPMALLGNREEKLPDTSPKGLTGKK
LGVEANSLYQAYAEDHFDHSQIQTYASLEEAILDLGEGRIDLVLGNKADISDFLKNRREAQNTAILGELPHDAAYLGEGI
GIGLRKEDKELKRQFDQALEDINKDGTFTKIRERYFPYAIN

Specific function: Component of the nopaline active transport system probably consisting of four subunits:Q, M, P and T. This system is also capable of transporting octopine provided that catabolic functions are induced with nopaline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=232, Percent_Identity=35.3448275862069, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI1787085, Length=227, Percent_Identity=31.2775330396476, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788649, Length=255, Percent_Identity=32.156862745098, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=30.3030303030303, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788228, Length=225, Percent_Identity=27.5555555555556, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787031, Length=228, Percent_Identity=25.4385964912281, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31695; Mature: 31695

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIDFPFSLHTLIRRHQEGLGTGVLTRFLVYSFLAIVLMSSPCRATDTPGPELRIASEGA
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCC
RPPYNYLENNELAGFEIDLGQELCKRMNRVCRFVPQDWDNLIPGLLDHRYDAIMAALDIS
CCCHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DAAKEKIAFSEPYVRMPMALLGNREEKLPDTSPKGLTGKKLGVEANSLYQAYAEDHFDHS
HHHHHHHHCCCCHHHCCHHHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
QIQTYASLEEAILDLGEGRIDLVLGNKADISDFLKNRREAQNTAILGELPHDAAYLGEGI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEECCCCCHHHHHCCCC
GIGLRKEDKELKRQFDQALEDINKDGTFTKIRERYFPYAIN
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MQIDFPFSLHTLIRRHQEGLGTGVLTRFLVYSFLAIVLMSSPCRATDTPGPELRIASEGA
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCC
RPPYNYLENNELAGFEIDLGQELCKRMNRVCRFVPQDWDNLIPGLLDHRYDAIMAALDIS
CCCHHHHCCCCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DAAKEKIAFSEPYVRMPMALLGNREEKLPDTSPKGLTGKKLGVEANSLYQAYAEDHFDHS
HHHHHHHHCCCCHHHCCHHHHCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
QIQTYASLEEAILDLGEGRIDLVLGNKADISDFLKNRREAQNTAILGELPHDAAYLGEGI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEECCCCCHHHHHCCCC
GIGLRKEDKELKRQFDQALEDINKDGTFTKIRERYFPYAIN
CCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1732218; 11743193; 11743194 [H]