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The map label for this gene is glxR [C]

Identifier: 182678759

GI number: 182678759

Start: 2026970

End: 2027845

Strand: Direct

Name: glxR [C]

Synonym: Bind_1788

Alternate gene names: 182678759

Gene position: 2026970-2027845 (Clockwise)

Preceding gene: 182678758

Following gene: 182678763

Centisome position: 48.61

GC content: 58.79

Gene sequence:

>876_bases
ATGGCAAAAGTCGCTTTTCTCGGTCTCGGCGTCATGGGTTATCCCATGGCCGGCCATCTCGTGAAACACGGTCACGAGGT
CACCGTCTATAATCGCAGTGCGGCGAAAGCCGAAAAATGGGCGACTCAATTCGGCGGCAAAACGGCCGCGACGCCAGGGG
CGGCGGCGGCGGGCCAGGAGATTGTTTTTTCCTGTGTCGGCAATGACGATGACGTGCGGATGATCACGATTGGCGAGAAC
GGCGCCTTTTCGACCCTGCCCGTCGGCGCGCTTTTCGTCGATCACACCACGGCTTCCGCCTCGGTCGCGCGTGAGCTTGC
CGCCATTGCCACTAAAGAAGGCAAACATTTTATCGATGCGCCCGTTTCGGGTGGTCAAGCGGGGGCTGAAAATGGTGCCC
TGACGGTCATGTGCGGCGGCGATGAAAAACCCTATGCGGAGGCCGAAAAGGTCATCAAGGCCTATGCTAAATCCTGCCGC
CTCATGGGACCGGCCGGATCAGGCCAATTGACCAAGATGGTCAACCAGATCTGCATCGCCGGACTGGTTCAGGCTCTTTC
CGAGGGATTGCATTTCGCGCAATGCGCCGGGCTCGACGGCGCGGCTGTCATCGATGTGATTTCGAAAGGCGCGGCGCAAT
CCTGGCAGATGGAGAACCGTTATAAAACCATGCTGGACGGCAAGTTCGATTTCGGTTTCGCCGTGCAATGGATGCGCAAG
GATCTTTCGATCTGCCTCGCCGAGGCCCGCAATAATGGCGCGAGCCTGCCGGTCTCGGCTTTGGTCGATCAATTCTATGC
CGAAGTCGAGAAACTCGGCGGGTCACGTTGGGATACATCGAGCCTGATCGCGCGGCTGCAACCAAAAGCCGGTTGA

Upstream 100 bases:

>100_bases
ATCAGCGGGGGAGCGCTTCAGCTCGATCACGCTCCCTAAGAACAATTCAAATGCGCGCGATACCGATACGTCGAAGCGCA
TGAACATAAGAGGACGTCCA

Downstream 100 bases:

>100_bases
GGGCAAAGCCAAAACAGACAAGAGGATGCCAGCGATAGCTCGGGCATCCTCGAACCTGCCGATGGCGCAGTCACCGCTTC
AGTCTGCGCTCTCGGCCTGA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAKVAFLGLGVMGYPMAGHLVKHGHEVTVYNRSAAKAEKWATQFGGKTAATPGAAAAGQEIVFSCVGNDDDVRMITIGEN
GAFSTLPVGALFVDHTTASASVARELAAIATKEGKHFIDAPVSGGQAGAENGALTVMCGGDEKPYAEAEKVIKAYAKSCR
LMGPAGSGQLTKMVNQICIAGLVQALSEGLHFAQCAGLDGAAVIDVISKGAAQSWQMENRYKTMLDGKFDFGFAVQWMRK
DLSICLAEARNNGASLPVSALVDQFYAEVEKLGGSRWDTSSLIARLQPKAG

Sequences:

>Translated_291_residues
MAKVAFLGLGVMGYPMAGHLVKHGHEVTVYNRSAAKAEKWATQFGGKTAATPGAAAAGQEIVFSCVGNDDDVRMITIGEN
GAFSTLPVGALFVDHTTASASVARELAAIATKEGKHFIDAPVSGGQAGAENGALTVMCGGDEKPYAEAEKVIKAYAKSCR
LMGPAGSGQLTKMVNQICIAGLVQALSEGLHFAQCAGLDGAAVIDVISKGAAQSWQMENRYKTMLDGKFDFGFAVQWMRK
DLSICLAEARNNGASLPVSALVDQFYAEVEKLGGSRWDTSSLIARLQPKAG
>Mature_290_residues
AKVAFLGLGVMGYPMAGHLVKHGHEVTVYNRSAAKAEKWATQFGGKTAATPGAAAAGQEIVFSCVGNDDDVRMITIGENG
AFSTLPVGALFVDHTTASASVARELAAIATKEGKHFIDAPVSGGQAGAENGALTVMCGGDEKPYAEAEKVIKAYAKSCRL
MGPAGSGQLTKMVNQICIAGLVQALSEGLHFAQCAGLDGAAVIDVISKGAAQSWQMENRYKTMLDGKFDFGFAVQWMRKD
LSICLAEARNNGASLPVSALVDQFYAEVEKLGGSRWDTSSLIARLQPKAG

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=27.8571428571429, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=27.4914089347079, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=31.9298245614035, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=32.1428571428571, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=28.0821917808219, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789092, Length=286, Percent_Identity=29.020979020979, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557316, Length=274, Percent_Identity=28.8321167883212, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28574115, Length=281, Percent_Identity=23.8434163701068, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24655230, Length=277, Percent_Identity=27.7978339350181, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI19922568, Length=277, Percent_Identity=27.7978339350181, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30407; Mature: 30276

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFLGLGVMGYPMAGHLVKHGHEVTVYNRSAAKAEKWATQFGGKTAATPGAAAAGQE
CCEEEEEECCHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCE
IVFSCVGNDDDVRMITIGENGAFSTLPVGALFVDHTTASASVARELAAIATKEGKHFIDA
EEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEC
PVSGGQAGAENGALTVMCGGDEKPYAEAEKVIKAYAKSCRLMGPAGSGQLTKMVNQICIA
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHH
GLVQALSEGLHFAQCAGLDGAAVIDVISKGAAQSWQMENRYKTMLDGKFDFGFAVQWMRK
HHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DLSICLAEARNNGASLPVSALVDQFYAEVEKLGGSRWDTSSLIARLQPKAG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
AKVAFLGLGVMGYPMAGHLVKHGHEVTVYNRSAAKAEKWATQFGGKTAATPGAAAAGQE
CEEEEEECCHHCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCE
IVFSCVGNDDDVRMITIGENGAFSTLPVGALFVDHTTASASVARELAAIATKEGKHFIDA
EEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEC
PVSGGQAGAENGALTVMCGGDEKPYAEAEKVIKAYAKSCRLMGPAGSGQLTKMVNQICIA
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHH
GLVQALSEGLHFAQCAGLDGAAVIDVISKGAAQSWQMENRYKTMLDGKFDFGFAVQWMRK
HHHHHHHHCCCCHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
DLSICLAEARNNGASLPVSALVDQFYAEVEKLGGSRWDTSSLIARLQPKAG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA