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The map label for this gene is ispE [H]

Identifier: 182677851

GI number: 182677851

Start: 978394

End: 979338

Strand: Direct

Name: ispE [H]

Synonym: Bind_0858

Alternate gene names: 182677851

Gene position: 978394-979338 (Clockwise)

Preceding gene: 182677850

Following gene: 182677852

Centisome position: 23.46

GC content: 62.54

Gene sequence:

>945_bases
TTGTTTTCGTTCCATGGCTCGAACCCGTTCCGTTCGGGCCATGCCCGCCCTTGGATCCTGCCTTTGTCCTCTCCCGCCGT
CCTGGTCGAGCGCGCGCCCGCGAAGATCAATCTCACCTTGCATGTGCTCGGCCGCAGTGATGATGGCTGGCATCAGCTCG
AAAGCCTTGTCGCTTTTTCCTGTGGCGGGGATCAGCTTTCCCTGACGCCTGGCGAGCCGCTTGGCCTGACGCTTGAAGGA
CCGACGGCTCCCTTGGCCGGCGACAATGATCAGAATCTGGTTCTCAAGGCGGCTCGCAATCTCGGCCAGCAGATCGAAAA
TCTGAAATTCGGCGCCTTTCATCTCGTCAAACGCCTCCCGGTTGCGGCGGGCATTGGTGGCGGTTCGTCGGATGCGGCGG
CAGCCCTTCGCCTTTTGGCGCGGACCAATGGGATCCCCCTCGATGATCCGCGCCTGATGGCCGCAGCGCGCGCGACTGGC
GCCGATGTCCCCGTGTGTCTCGAACGGCGGGCACGCATGATGCGCGGTGTCGGCGAGCAACTTGGCGAGCCGCTTCCTTT
GCCTCCGCTCGTCGGGTTGATTGTCAATCCGGGGCAGGCGCTCGAAACCAAGGCCGTGTTTTCCCGCATGAATCTCACGC
CTGGATCGCAGACCCATTATGGCCCGCATCCGGCTTTTTCTCCGAACCTTGACAGCTCTGGCTTCCTCGCCCTTCTGCGC
AAGGGCCGTAACGATATGGAAGACGCGGCCTCGGTCCTGGCCCCGGTCATTGGCGATGTGCTGGCCATTCTCTCGGCGGC
GCGTGGTTGCCAGCTCGCGCGCATGTCCGGGTCCGGCGCGACCTGTTTTGCGCTTTTTTCCGATTGTCGCGCCGCCGCCA
AGGCCAAGAAAGTGATTTTGCAAGCGCATCCCAACTGGTGGGTCAAGAGCTGCGCCTTTCGATAA

Upstream 100 bases:

>100_bases
AGGACAAGTGGATAAGTTTAAAAAGTGACGTATCGAGAATCCGTGATTCTCGATACTAGTCTCGTGCCTTGACCTTTCCT
TCCTGACCGGAAAAGGAAAA

Downstream 100 bases:

>100_bases
ACTGGCGCTTTTTCAAAGAGATAAGATTGTCTGTAAATTCTATAAAAAGCCTTTTAAAATGAAAAGTTATGGCTAAGCTC
TTGCTGAGCTATATAGGGGG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MFSFHGSNPFRSGHARPWILPLSSPAVLVERAPAKINLTLHVLGRSDDGWHQLESLVAFSCGGDQLSLTPGEPLGLTLEG
PTAPLAGDNDQNLVLKAARNLGQQIENLKFGAFHLVKRLPVAAGIGGGSSDAAAALRLLARTNGIPLDDPRLMAAARATG
ADVPVCLERRARMMRGVGEQLGEPLPLPPLVGLIVNPGQALETKAVFSRMNLTPGSQTHYGPHPAFSPNLDSSGFLALLR
KGRNDMEDAASVLAPVIGDVLAILSAARGCQLARMSGSGATCFALFSDCRAAAKAKKVILQAHPNWWVKSCAFR

Sequences:

>Translated_314_residues
MFSFHGSNPFRSGHARPWILPLSSPAVLVERAPAKINLTLHVLGRSDDGWHQLESLVAFSCGGDQLSLTPGEPLGLTLEG
PTAPLAGDNDQNLVLKAARNLGQQIENLKFGAFHLVKRLPVAAGIGGGSSDAAAALRLLARTNGIPLDDPRLMAAARATG
ADVPVCLERRARMMRGVGEQLGEPLPLPPLVGLIVNPGQALETKAVFSRMNLTPGSQTHYGPHPAFSPNLDSSGFLALLR
KGRNDMEDAASVLAPVIGDVLAILSAARGCQLARMSGSGATCFALFSDCRAAAKAKKVILQAHPNWWVKSCAFR
>Mature_314_residues
MFSFHGSNPFRSGHARPWILPLSSPAVLVERAPAKINLTLHVLGRSDDGWHQLESLVAFSCGGDQLSLTPGEPLGLTLEG
PTAPLAGDNDQNLVLKAARNLGQQIENLKFGAFHLVKRLPVAAGIGGGSSDAAAALRLLARTNGIPLDDPRLMAAARATG
ADVPVCLERRARMMRGVGEQLGEPLPLPPLVGLIVNPGQALETKAVFSRMNLTPGSQTHYGPHPAFSPNLDSSGFLALLR
KGRNDMEDAASVLAPVIGDVLAILSAARGCQLARMSGSGATCFALFSDCRAAAKAKKVILQAHPNWWVKSCAFR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=282, Percent_Identity=30.4964539007092, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 33122; Mature: 33122

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFHGSNPFRSGHARPWILPLSSPAVLVERAPAKINLTLHVLGRSDDGWHQLESLVAFS
CCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHC
CGGDQLSLTPGEPLGLTLEGPTAPLAGDNDQNLVLKAARNLGQQIENLKFGAFHLVKRLP
CCCCEEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
VAAGIGGGSSDAAAALRLLARTNGIPLDDPRLMAAARATGADVPVCLERRARMMRGVGEQ
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LGEPLPLPPLVGLIVNPGQALETKAVFSRMNLTPGSQTHYGPHPAFSPNLDSSGFLALLR
HCCCCCCCHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KGRNDMEDAASVLAPVIGDVLAILSAARGCQLARMSGSGATCFALFSDCRAAAKAKKVIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEE
QAHPNWWVKSCAFR
EECCCHHHHHCCCC
>Mature Secondary Structure
MFSFHGSNPFRSGHARPWILPLSSPAVLVERAPAKINLTLHVLGRSDDGWHQLESLVAFS
CCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEEEEEEECCCCHHHHHHHHHHHC
CGGDQLSLTPGEPLGLTLEGPTAPLAGDNDQNLVLKAARNLGQQIENLKFGAFHLVKRLP
CCCCEEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
VAAGIGGGSSDAAAALRLLARTNGIPLDDPRLMAAARATGADVPVCLERRARMMRGVGEQ
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LGEPLPLPPLVGLIVNPGQALETKAVFSRMNLTPGSQTHYGPHPAFSPNLDSSGFLALLR
HCCCCCCCHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KGRNDMEDAASVLAPVIGDVLAILSAARGCQLARMSGSGATCFALFSDCRAAAKAKKVIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEEE
QAHPNWWVKSCAFR
EECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA