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The map label for this gene is nadE [H]

Identifier: 182677746

GI number: 182677746

Start: 844546

End: 846219

Strand: Direct

Name: nadE [H]

Synonym: Bind_0753

Alternate gene names: 182677746

Gene position: 844546-846219 (Clockwise)

Preceding gene: 182677745

Following gene: 182677750

Centisome position: 20.25

GC content: 57.11

Gene sequence:

>1674_bases
ATGAGCGACACGCGTCCAGTGGATCGCCTCGTCATCGCGCTCGCGCAGATCAATTCGACAGTCGGTGATATCGACGGCAA
TCTCGCCCGTATTCGAGAGGCACGCGCCAAGGCGCACGGCTTCGGCGCTGATCTCGTCATGTTTTCTGAGCTCTTCATCT
CCGGCTATCCCCCGGAGGATCTGGTTCTGAAACCCAGCTTTCAGGACGCCTGCCGCGTCGCTTGTGAAAATCTTGCCCGC
GAGACGGCGGATGGTGGCCCAGCCATTCTCGTTGGCTTGCCCTGGGGCGAATTGGGCGCCTGCTATAATGCTTATGCGCT
GCTCGCTCATGGCCGGATTGAAGCCATGAGGTTCAAGGTCGATTTGCCGAATTATGGCGTTTTCGACGAAAAGCGCGTTT
TCCGTGCGGGTCCCTTGCAGGGGCCCATTATCTTCAAGGGCGTGCGTATCGGCATTCCGATCTGCGAGGATATTTGGGGA
CCTGATCCCGTCGAATGCATTGCCGAAACGGGTGGTGAAATCCTGCTCGTGCCGAATGGCTCGCCTTATTGGGAATCGAA
GAGCAGCGAGCGTTTGTCAGTCGTCAAGGCACGGATCAGTGAGACCCATCTGCCTTTGATCTATCTGAACCAGCTCGGCG
GTCAGGACGAATTAGTCTTTGATGGCGCCACTTTCGCGTTAAACGCGGATGGAACACTCGCCTGCCAGTTGCCGGCTTTC
GAGGCCATTATCGCCAAAACCGTCTGGGAACGCCGGGGCGATACATTCGTCTGCGTCGAGGCACCGAAGACACAAGTGAT
TGAGGGTGACGACGCGGATTACACGGCCTGTATGCTGGGCCTGCGGGATTATGTCGAAAAAATCGGTTTTCCTGGTGTCG
TGCTGGGCCTCTCTGGCGGTGTCGATTCCGCGCTTTGCGCGGCGCTCGCTGTCGATGCCCTGGGGCGTGAGAAGGTTCAT
GCCGTCATGCTGCCCTATCGTTACACCTCCCAGGAATCCTTGATCGATGCCAAGGCCTGCGCCACAGCGCTTGGTTTGCG
TTATGATATCGTACCCATTGCGCCCGCGGTTGAAGGTTTCGAGACGTTGCTCGCGCCGATGTTCGCCGGTACGGCGCCGG
GGTTGACGGAGGAAAATCTGCAGAGCCGTACACGTGGCACAGTGCTGATGTCGATCTCCAATAAATTTGGCTCCATGCTG
GTCACGACGGGTAACAAGTCGGAAATGTCGGTGGGCTATGCGACGATCTATGGCGACATGAACGGCGGCTTCAATCCGAT
CAAGGATATTTACAAAACGCAGGTGTTTCGCCTTTGTCATTTGCGCAATCGCTGGAAGCCAAAGGGTGCGCTCGGACCTG
ACGGTGAAGTCATTCCGTATAATATTATCGTCAAGCCGCCATCCGCCGAATTGCGCGAAAATCAGAAGGATCAGGATTCA
CTGCCTCCTTATGACATCCTCGATGGGATCCTCGAAGGTCTCGTCGAGAAGGATCAAGGCGTCGCGGATCTGGTCGCTGC
GGGTTATGAGGAACCCACAATACGCCGGGTGGAGCGCCTTCTTTACCTGGCGGAATATAAAAGGCGGCAATCGGCGCCGG
GCGTAAAGGTGACGCGCCGCAATTTCGGGCGGGACCGCCGCTATCCGATCGTCAATGGTTTCCGGGATCGGTAG

Upstream 100 bases:

>100_bases
TTTACAGAAAGAGAGAGCATCGGGCAGGATTCAATTTGAAAGTCCGATGCTCTAGAGCATTCTCAAGCAAGTCCCGAGAT
TGGAAGAGGAGCCGGCAGGC

Downstream 100 bases:

>100_bases
AAAGCTTGGGCATGATACCGGAAAGCGGTCCCGCTATCGGAGCGAATGATAGCCCGATAGTCGCCGATCTAAATCCTGGA
AGCGCACTCGTTGGTCAGCA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSDTRPVDRLVIALAQINSTVGDIDGNLARIREARAKAHGFGADLVMFSELFISGYPPEDLVLKPSFQDACRVACENLAR
ETADGGPAILVGLPWGELGACYNAYALLAHGRIEAMRFKVDLPNYGVFDEKRVFRAGPLQGPIIFKGVRIGIPICEDIWG
PDPVECIAETGGEILLVPNGSPYWESKSSERLSVVKARISETHLPLIYLNQLGGQDELVFDGATFALNADGTLACQLPAF
EAIIAKTVWERRGDTFVCVEAPKTQVIEGDDADYTACMLGLRDYVEKIGFPGVVLGLSGGVDSALCAALAVDALGREKVH
AVMLPYRYTSQESLIDAKACATALGLRYDIVPIAPAVEGFETLLAPMFAGTAPGLTEENLQSRTRGTVLMSISNKFGSML
VTTGNKSEMSVGYATIYGDMNGGFNPIKDIYKTQVFRLCHLRNRWKPKGALGPDGEVIPYNIIVKPPSAELRENQKDQDS
LPPYDILDGILEGLVEKDQGVADLVAAGYEEPTIRRVERLLYLAEYKRRQSAPGVKVTRRNFGRDRRYPIVNGFRDR

Sequences:

>Translated_557_residues
MSDTRPVDRLVIALAQINSTVGDIDGNLARIREARAKAHGFGADLVMFSELFISGYPPEDLVLKPSFQDACRVACENLAR
ETADGGPAILVGLPWGELGACYNAYALLAHGRIEAMRFKVDLPNYGVFDEKRVFRAGPLQGPIIFKGVRIGIPICEDIWG
PDPVECIAETGGEILLVPNGSPYWESKSSERLSVVKARISETHLPLIYLNQLGGQDELVFDGATFALNADGTLACQLPAF
EAIIAKTVWERRGDTFVCVEAPKTQVIEGDDADYTACMLGLRDYVEKIGFPGVVLGLSGGVDSALCAALAVDALGREKVH
AVMLPYRYTSQESLIDAKACATALGLRYDIVPIAPAVEGFETLLAPMFAGTAPGLTEENLQSRTRGTVLMSISNKFGSML
VTTGNKSEMSVGYATIYGDMNGGFNPIKDIYKTQVFRLCHLRNRWKPKGALGPDGEVIPYNIIVKPPSAELRENQKDQDS
LPPYDILDGILEGLVEKDQGVADLVAAGYEEPTIRRVERLLYLAEYKRRQSAPGVKVTRRNFGRDRRYPIVNGFRDR
>Mature_556_residues
SDTRPVDRLVIALAQINSTVGDIDGNLARIREARAKAHGFGADLVMFSELFISGYPPEDLVLKPSFQDACRVACENLARE
TADGGPAILVGLPWGELGACYNAYALLAHGRIEAMRFKVDLPNYGVFDEKRVFRAGPLQGPIIFKGVRIGIPICEDIWGP
DPVECIAETGGEILLVPNGSPYWESKSSERLSVVKARISETHLPLIYLNQLGGQDELVFDGATFALNADGTLACQLPAFE
AIIAKTVWERRGDTFVCVEAPKTQVIEGDDADYTACMLGLRDYVEKIGFPGVVLGLSGGVDSALCAALAVDALGREKVHA
VMLPYRYTSQESLIDAKACATALGLRYDIVPIAPAVEGFETLLAPMFAGTAPGLTEENLQSRTRGTVLMSISNKFGSMLV
TTGNKSEMSVGYATIYGDMNGGFNPIKDIYKTQVFRLCHLRNRWKPKGALGPDGEVIPYNIIVKPPSAELRENQKDQDSL
PPYDILDGILEGLVEKDQGVADLVAAGYEEPTIRRVERLLYLAEYKRRQSAPGVKVTRRNFGRDRRYPIVNGFRDR

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=267, Percent_Identity=28.4644194756554, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60839; Mature: 60708

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTRPVDRLVIALAQINSTVGDIDGNLARIREARAKAHGFGADLVMFSELFISGYPPED
CCCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
LVLKPSFQDACRVACENLARETADGGPAILVGLPWGELGACYNAYALLAHGRIEAMRFKV
CEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEE
DLPNYGVFDEKRVFRAGPLQGPIIFKGVRIGIPICEDIWGPDPVECIAETGGEILLVPNG
ECCCCCCCCCCHHHCCCCCCCCEEEEEEEECCCHHHHCCCCCHHHHHHHCCCCEEEECCC
SPYWESKSSERLSVVKARISETHLPLIYLNQLGGQDELVFDGATFALNADGTLACQLPAF
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCEEEEECCCCEEEEECCHH
EAIIAKTVWERRGDTFVCVEAPKTQVIEGDDADYTACMLGLRDYVEKIGFPGVVLGLSGG
HHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
VDSALCAALAVDALGREKVHAVMLPYRYTSQESLIDAKACATALGLRYDIVPIAPAVEGF
CHHHHHHHHHHHHCCCCCEEEEEECEEECCHHHHHHHHHHHHHHCCEEEEEECCHHHHHH
ETLLAPMFAGTAPGLTEENLQSRTRGTVLMSISNKFGSMLVTTGNKSEMSVGYATIYGDM
HHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCCEEEECCCCCCCEEEEEEEEECC
NGGFNPIKDIYKTQVFRLCHLRNRWKPKGALGPDGEVIPYNIIVKPPSAELRENQKDQDS
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHHHCCCCCCCC
LPPYDILDGILEGLVEKDQGVADLVAAGYEEPTIRRVERLLYLAEYKRRQSAPGVKVTRR
CCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHH
NFGRDRRYPIVNGFRDR
HCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDTRPVDRLVIALAQINSTVGDIDGNLARIREARAKAHGFGADLVMFSELFISGYPPED
CCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
LVLKPSFQDACRVACENLARETADGGPAILVGLPWGELGACYNAYALLAHGRIEAMRFKV
CEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEE
DLPNYGVFDEKRVFRAGPLQGPIIFKGVRIGIPICEDIWGPDPVECIAETGGEILLVPNG
ECCCCCCCCCCHHHCCCCCCCCEEEEEEEECCCHHHHCCCCCHHHHHHHCCCCEEEECCC
SPYWESKSSERLSVVKARISETHLPLIYLNQLGGQDELVFDGATFALNADGTLACQLPAF
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCEEEEECCCCEEEEECCHH
EAIIAKTVWERRGDTFVCVEAPKTQVIEGDDADYTACMLGLRDYVEKIGFPGVVLGLSGG
HHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
VDSALCAALAVDALGREKVHAVMLPYRYTSQESLIDAKACATALGLRYDIVPIAPAVEGF
CHHHHHHHHHHHHCCCCCEEEEEECEEECCHHHHHHHHHHHHHHCCEEEEEECCHHHHHH
ETLLAPMFAGTAPGLTEENLQSRTRGTVLMSISNKFGSMLVTTGNKSEMSVGYATIYGDM
HHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCCEEEECCCCCCCEEEEEEEEECC
NGGFNPIKDIYKTQVFRLCHLRNRWKPKGALGPDGEVIPYNIIVKPPSAELRENQKDQDS
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCHHHHCCCCCCCC
LPPYDILDGILEGLVEKDQGVADLVAAGYEEPTIRRVERLLYLAEYKRRQSAPGVKVTRR
CCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHH
NFGRDRRYPIVNGFRDR
HCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]