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The map label for this gene is ruvC

Identifier: 182677006

GI number: 182677006

Start: 3144

End: 3671

Strand: Direct

Name: ruvC

Synonym: Bind_0004

Alternate gene names: 182677006

Gene position: 3144-3671 (Clockwise)

Preceding gene: 182677005

Following gene: 182677007

Centisome position: 0.08

GC content: 59.85

Gene sequence:

>528_bases
ATGATGCCGCCGCGCCTGCGCATCCTCGGCATTGACCCTGGCTTGCGCAATATGGGCTGGGGCATTGTCGAACTTCTGGG
TTCGAATCTCACCTATGTGGCAAGCGGCGTCGTGCATTCGGATGGCAAGGGTGAACTCGCCCATCGCTTGAAGGATTTGC
ACGTGGGGCTCGCCGGTGTCATCGACGCCTATACACCGCATGAAGCCGCGATCGAGGAGACGTTCGTCAATCGCGATCCG
CAATCGACCTTGAAGCTCGGGCAGGCACGCGGAATCGCTCTGGTGGTGCCGGCACTGGCCGATCTCGCGGTCGCCGAATA
TGCCGCCAATCTCATCAAGAAAACAGTGACGGGCAATGGCCATGCGGAAAAGCAACAGGTCGCCATGATGGTCAAGATCC
TGCTGCCCAAAAGCGATGCCGGCAGCGCTGATGCGGTCGATGCGCTCGCGGTCGCCATTACCCATGCGCAATTGCGGCGC
AATCCCGCTGCTCTCAAAAGCCAAGCTATGATTGGTCAGGGGCGATGA

Upstream 100 bases:

>100_bases
AGATTCTTTCAGCTTGTGAGAGACTCATGCCCGCCAGAAAGTCCGCCGCGTCTTTCTCCTCCAGATCGTGCGGAAGACAA
TCTGGCGGGAGGAAATCCGC

Downstream 100 bases:

>100_bases
TCGGCAAGCTGACCGGCCTCGTGGATAGCCAAGGGGAGGATCATGTGATCCTCGACGTGCAGGGCGTCGGCTACATCGTC
TTTTGTTCGAGCCGGACTTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MMPPRLRILGIDPGLRNMGWGIVELLGSNLTYVASGVVHSDGKGELAHRLKDLHVGLAGVIDAYTPHEAAIEETFVNRDP
QSTLKLGQARGIALVVPALADLAVAEYAANLIKKTVTGNGHAEKQQVAMMVKILLPKSDAGSADAVDALAVAITHAQLRR
NPAALKSQAMIGQGR

Sequences:

>Translated_175_residues
MMPPRLRILGIDPGLRNMGWGIVELLGSNLTYVASGVVHSDGKGELAHRLKDLHVGLAGVIDAYTPHEAAIEETFVNRDP
QSTLKLGQARGIALVVPALADLAVAEYAANLIKKTVTGNGHAEKQQVAMMVKILLPKSDAGSADAVDALAVAITHAQLRR
NPAALKSQAMIGQGR
>Mature_175_residues
MMPPRLRILGIDPGLRNMGWGIVELLGSNLTYVASGVVHSDGKGELAHRLKDLHVGLAGVIDAYTPHEAAIEETFVNRDP
QSTLKLGQARGIALVVPALADLAVAEYAANLIKKTVTGNGHAEKQQVAMMVKILLPKSDAGSADAVDALAVAITHAQLRR
NPAALKSQAMIGQGR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=169, Percent_Identity=42.603550295858, Blast_Score=110, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_BEII9 (B2IAW3)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831152.1
- GeneID:   6201463
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0004
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18439; Mature: 18439

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPPRLRILGIDPGLRNMGWGIVELLGSNLTYVASGVVHSDGKGELAHRLKDLHVGLAGV
CCCCCEEEEECCCCCCHHCHHHHHHHCCCHHEEECCEEECCCCHHHHHHHHHHHHHHHHH
IDAYTPHEAAIEETFVNRDPQSTLKLGQARGIALVVPALADLAVAEYAANLIKKTVTGNG
HHCCCCCHHHHHHHHCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCC
HAEKQQVAMMVKILLPKSDAGSADAVDALAVAITHAQLRRNPAALKSQAMIGQGR
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MMPPRLRILGIDPGLRNMGWGIVELLGSNLTYVASGVVHSDGKGELAHRLKDLHVGLAGV
CCCCCEEEEECCCCCCHHCHHHHHHHCCCHHEEECCEEECCCCHHHHHHHHHHHHHHHHH
IDAYTPHEAAIEETFVNRDPQSTLKLGQARGIALVVPALADLAVAEYAANLIKKTVTGNG
HHCCCCCHHHHHHHHCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCC
HAEKQQVAMMVKILLPKSDAGSADAVDALAVAITHAQLRRNPAALKSQAMIGQGR
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA