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The map label for this gene is vsr [H]

Identifier: 17548156

GI number: 17548156

Start: 3710105

End: 3710569

Strand: Reverse

Name: vsr [H]

Synonym: RSc3439

Alternate gene names: 17548156

Gene position: 3710569-3710105 (Counterclockwise)

Preceding gene: 17548157

Following gene: 17548155

Centisome position: 99.84

GC content: 52.9

Gene sequence:

>465_bases
ATGGACACCATATCGAAGGTGGCTCGCAGTGCCCTTATGGCAAGAATCAGAAACAAAGACTCGAAGCCAGAACGCGCTTT
ACGCTCAGCCATGCATCGGACGGGGCTCCGTTTTCGACTTCATCGCACGGATCTCCCTGGGACGCCCGACATTGTTCTTC
CCGCAACGAAAATTGCGATCTTTGTTCACGGATGTTTCTGGCATCAACATGCGCGATGCAAGCTCGCGTCGTCGCCCCGG
AGCAATACCGAATACTGGCTTCCAAAACTACAGCGTAACGTTGAGCGGGATCGGCGCGTCGCTAAAGCTCTGCGGCAGTT
AGGCTGGGCAGTTCTCACTGTATGGGAATGCCAAACACGTAAAGAAATCTCGCTCGCGGTGGCTTTGAAACGAATACACA
CCAGCGTACGAAAAAGAAAGGATAAAAATGCTACTCAGTCTATTCTGCGGCGCAGGAGGGCTTGA

Upstream 100 bases:

>100_bases
CGCGGAATATTGATGTTTGAGGGGTTGGTTCACTCAGACGATATGGAGTGAGCGGGCAGCGCGTTAGTTGTATCTAGTCT
GGCATTGAGGGGGCGTTCGT

Downstream 100 bases:

>100_bases
CAAGGGTTTCGAACAAGCGGGCTTTGAAGTGGGTTTGGCATTCGATATCCGGCCGGACAGCATCGAATCATACAACCGCA
ATCGGCGTGCGCCAATCAGA

Product: patch repair protein

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MDTISKVARSALMARIRNKDSKPERALRSAMHRTGLRFRLHRTDLPGTPDIVLPATKIAIFVHGCFWHQHARCKLASSPR
SNTEYWLPKLQRNVERDRRVAKALRQLGWAVLTVWECQTRKEISLAVALKRIHTSVRKRKDKNATQSILRRRRA

Sequences:

>Translated_154_residues
MDTISKVARSALMARIRNKDSKPERALRSAMHRTGLRFRLHRTDLPGTPDIVLPATKIAIFVHGCFWHQHARCKLASSPR
SNTEYWLPKLQRNVERDRRVAKALRQLGWAVLTVWECQTRKEISLAVALKRIHTSVRKRKDKNATQSILRRRRA
>Mature_154_residues
MDTISKVARSALMARIRNKDSKPERALRSAMHRTGLRFRLHRTDLPGTPDIVLPATKIAIFVHGCFWHQHARCKLASSPR
SNTEYWLPKLQRNVERDRRVAKALRQLGWAVLTVWECQTRKEISLAVALKRIHTSVRKRKDKNATQSILRRRRA

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=119, Percent_Identity=46.218487394958, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 17938; Mature: 17938

Theoretical pI: Translated: 12.23; Mature: 12.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTISKVARSALMARIRNKDSKPERALRSAMHRTGLRFRLHRTDLPGTPDIVLPATKIAI
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEHHHHHHH
FVHGCFWHQHARCKLASSPRSNTEYWLPKLQRNVERDRRVAKALRQLGWAVLTVWECQTR
HHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KEISLAVALKRIHTSVRKRKDKNATQSILRRRRA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MDTISKVARSALMARIRNKDSKPERALRSAMHRTGLRFRLHRTDLPGTPDIVLPATKIAI
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEHHHHHHH
FVHGCFWHQHARCKLASSPRSNTEYWLPKLQRNVERDRRVAKALRQLGWAVLTVWECQTR
HHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KEISLAVALKRIHTSVRKRKDKNATQSILRRRRA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]