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The map label for this gene is mthTIM [H]

Identifier: 17548155

GI number: 17548155

Start: 3709048

End: 3710142

Strand: Reverse

Name: mthTIM [H]

Synonym: RSc3438

Alternate gene names: 17548155

Gene position: 3710142-3709048 (Counterclockwise)

Preceding gene: 17548156

Following gene: 17548152

Centisome position: 99.83

GC content: 50.14

Gene sequence:

>1095_bases
ATGCTACTCAGTCTATTCTGCGGCGCAGGAGGGCTTGACAAGGGTTTCGAACAAGCGGGCTTTGAAGTGGGTTTGGCATT
CGATATCCGGCCGGACAGCATCGAATCATACAACCGCAATCGGCGTGCGCCAATCAGAGGGTATTGCCGAGATATTCGTG
ATATTAAACCCAAAGCGCTGGATGAGCTCTTTGGAGAGACCTTCCGCCCATCTGGCATCATTGGCGGTCCGCCTTGTCAA
AGCTTTAGCCGCGCCAATAAATCACAGAGCAATGATGATCCACGGCATGAGCTTCCATTTGTATATGCCGATCTTATTCG
GACGCTAAACAAACGATCGCCAGTTCCGTTCTTTGTTTTTGAGAACGTAGTTGGCCTCACGGAGGAGCCTCATAACGAGA
AATTTGTCGAGCTGAAGAAGCGCCTCGGTAAGATTGGCTTCTCTGTGCAAGAAGCAATACTGAATGCCGCCAACTATTCG
GTCCCTCAGAACCGGGAGCGGCTTATCATGGTTGGGTTTAATCGTGATCTCTATCCGGATCTGACTTGGCTGCCGCCTGG
CCCCAAAAACGCAAGACTAACCGTCAGGGAGGCAATAGGAGCGCTTCCGGAACCAACATATTTTCAGCGCAACTTGACCA
ACAAAGATATTCCGCATCACCCAAATCATTGGTGCATGCAACCCAAGTCGGCCAAATTTACGCAGCCAGGCGCTCTCGTT
GAGGGGCAACGTTCTCATCGAAGCTTTAAAACGCTAACATGGAATGAGCCCAGCATTACTGTTGCCTATGGACACCGAGA
AGTACATGTGCATCCAAATTGCCATCGGAGGTTAAGTGTCTATGAAGCGATGTTGCTCCAAGGATTTCCAACGGAATATG
TTCTTTATGGCAATCTTTCTTCGCAAATAACTCAAATCAGCGAGGCGGTCCCACCCCCTTTAGCGTATGCCGTGGCGATG
TCAATACAAGAACAGCTAAATTTAGTCAATGCCACCAGGCATGTTGAAATGGCGGCACAATGCCGCCTCAATTTGACTGC
TCGTCAACTGGCTGCTGTAGCGGTGCCCACCACTCTTTCGGCAAACTCCCGATAG

Upstream 100 bases:

>100_bases
GCAGTTCTCACTGTATGGGAATGCCAAACACGTAAAGAAATCTCGCTCGCGGTGGCTTTGAAACGAATACACACCAGCGT
ACGAAAAAGAAAGGATAAAA

Downstream 100 bases:

>100_bases
TTGTCTACGTTGTAACCTGCCAGCTTAAGAAAGCGCGCGGACTCCAGAATCATTGCATTCATTTCTGTCGGTGAACCTGC
CAAATGGTTATCTAGATCAA

Product: DNA modification cytosine-specific methyltransferase

Products: NA

Alternate protein names: M.MthTI; Cytosine-specific methyltransferase MthTI [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MLLSLFCGAGGLDKGFEQAGFEVGLAFDIRPDSIESYNRNRRAPIRGYCRDIRDIKPKALDELFGETFRPSGIIGGPPCQ
SFSRANKSQSNDDPRHELPFVYADLIRTLNKRSPVPFFVFENVVGLTEEPHNEKFVELKKRLGKIGFSVQEAILNAANYS
VPQNRERLIMVGFNRDLYPDLTWLPPGPKNARLTVREAIGALPEPTYFQRNLTNKDIPHHPNHWCMQPKSAKFTQPGALV
EGQRSHRSFKTLTWNEPSITVAYGHREVHVHPNCHRRLSVYEAMLLQGFPTEYVLYGNLSSQITQISEAVPPPLAYAVAM
SIQEQLNLVNATRHVEMAAQCRLNLTARQLAAVAVPTTLSANSR

Sequences:

>Translated_364_residues
MLLSLFCGAGGLDKGFEQAGFEVGLAFDIRPDSIESYNRNRRAPIRGYCRDIRDIKPKALDELFGETFRPSGIIGGPPCQ
SFSRANKSQSNDDPRHELPFVYADLIRTLNKRSPVPFFVFENVVGLTEEPHNEKFVELKKRLGKIGFSVQEAILNAANYS
VPQNRERLIMVGFNRDLYPDLTWLPPGPKNARLTVREAIGALPEPTYFQRNLTNKDIPHHPNHWCMQPKSAKFTQPGALV
EGQRSHRSFKTLTWNEPSITVAYGHREVHVHPNCHRRLSVYEAMLLQGFPTEYVLYGNLSSQITQISEAVPPPLAYAVAM
SIQEQLNLVNATRHVEMAAQCRLNLTARQLAAVAVPTTLSANSR
>Mature_364_residues
MLLSLFCGAGGLDKGFEQAGFEVGLAFDIRPDSIESYNRNRRAPIRGYCRDIRDIKPKALDELFGETFRPSGIIGGPPCQ
SFSRANKSQSNDDPRHELPFVYADLIRTLNKRSPVPFFVFENVVGLTEEPHNEKFVELKKRLGKIGFSVQEAILNAANYS
VPQNRERLIMVGFNRDLYPDLTWLPPGPKNARLTVREAIGALPEPTYFQRNLTNKDIPHHPNHWCMQPKSAKFTQPGALV
EGQRSHRSFKTLTWNEPSITVAYGHREVHVHPNCHRRLSVYEAMLLQGFPTEYVLYGNLSSQITQISEAVPPPLAYAVAM
SIQEQLNLVNATRHVEMAAQCRLNLTARQLAAVAVPTTLSANSR

Specific function: This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the MthTI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=201, Percent_Identity=29.3532338308458, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40946; Mature: 40946

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSLFCGAGGLDKGFEQAGFEVGLAFDIRPDSIESYNRNRRAPIRGYCRDIRDIKPKAL
CEEEEEECCCCCCCHHHHCCCEEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHH
DELFGETFRPSGIIGGPPCQSFSRANKSQSNDDPRHELPFVYADLIRTLNKRSPVPFFVF
HHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCEEEH
ENVVGLTEEPHNEKFVELKKRLGKIGFSVQEAILNAANYSVPQNRERLIMVGFNRDLYPD
HHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCC
LTWLPPGPKNARLTVREAIGALPEPTYFQRNLTNKDIPHHPNHWCMQPKSAKFTQPGALV
CCCCCCCCCCCEEEHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGQRSHRSFKTLTWNEPSITVAYGHREVHVHPNCHRRLSVYEAMLLQGFPTEYVLYGNLS
CCCHHCCCCCEEEECCCEEEEEECCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECHH
SQITQISEAVPPPLAYAVAMSIQEQLNLVNATRHVEMAAQCRLNLTARQLAAVAVPTTLS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
ANSR
CCCC
>Mature Secondary Structure
MLLSLFCGAGGLDKGFEQAGFEVGLAFDIRPDSIESYNRNRRAPIRGYCRDIRDIKPKAL
CEEEEEECCCCCCCHHHHCCCEEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHH
DELFGETFRPSGIIGGPPCQSFSRANKSQSNDDPRHELPFVYADLIRTLNKRSPVPFFVF
HHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCCEEEH
ENVVGLTEEPHNEKFVELKKRLGKIGFSVQEAILNAANYSVPQNRERLIMVGFNRDLYPD
HHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCC
LTWLPPGPKNARLTVREAIGALPEPTYFQRNLTNKDIPHHPNHWCMQPKSAKFTQPGALV
CCCCCCCCCCCEEEHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EGQRSHRSFKTLTWNEPSITVAYGHREVHVHPNCHRRLSVYEAMLLQGFPTEYVLYGNLS
CCCHHCCCCCEEEECCCEEEEEECCEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECHH
SQITQISEAVPPPLAYAVAMSIQEQLNLVNATRHVEMAAQCRLNLTARQLAAVAVPTTLS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
ANSR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1512204; 1336177 [H]