Click here to switch to the map view.

The map label for this gene is artJ [H]

Identifier: 17548127

GI number: 17548127

Start: 3686226

End: 3686993

Strand: Reverse

Name: artJ [H]

Synonym: RSc3410

Alternate gene names: 17548127

Gene position: 3686993-3686226 (Counterclockwise)

Preceding gene: 17548129

Following gene: 17548126

Centisome position: 99.21

GC content: 65.76

Gene sequence:

>768_bases
ATGTCTTTCGTCTCGTTTTCGCGCCGTGCCGCACTGGTTCTGTGCATGCTCGGCTCCGCCTCGCTCGCGTGGGCGCAAGG
CGGCGGTGCCCCCACCTACAACGTGGGCGCGACGGCCACCGGCGTGCCGTTCACCTTCCTGGACGTGAAGAGCAATTCCA
TCCAGGGGATGATGGTCGATACCGTCACCGCCGTGGGCAAGGCGGGCGGCTTCAGCGTGAACGTGCAGCAGACGGTGTTC
GCGGCGCTGATCCCGTCGCTGACGTCGAGCAAGATCGACATCATCTCGGCGGCCATGCTCAAGACGCCGGCGCGCGCGCA
GGTGGTGGATTTCTCCGACCCCGTGTACTCGTACGGCGAGGGCCTGCGGGTCAAGGCCGACGACGCCAAGACCTACGCCT
CGCTCGATGACCTGAAGGGCGAGGTGGTGGGCGCGCAGGTGGGCACGGTGTTCCTCGACATGCTCAACAAGAAGGGCATC
TTCAAGGAAGTGCGCAGCTACGACTCGGTGGCCGACATGACGCGCGACCTGGCGCTGGGCCGCATCAAGGCGGGCCTGGG
CGACCAGCCGATCCTCGCGTACCAGATCCGCCAGAACACCTTCCAGGGCGTGAAGCTGGTCTCGAGCTACAAACCCGTGA
ACGTGGGCGATGTGTGCCTGGTGGTGCGCAAGGGCGACGCCGAACTGCTCGGCCGCATCAACAAGGCCATCGCCAAGATC
AAGGCCGACGGCACGCTGGCGGCCATCGTGCAGAAGTGGGGCATCTGA

Upstream 100 bases:

>100_bases
CAATACGATCGATTATTGGCATGCCCATTGCATGAACGCGAACCCGTCCCCGCGTCGTCCCGTTCGGGGTGCGACTTTTC
CGATTGCCAGGAGGTGTCTC

Downstream 100 bases:

>100_bases
TCTCGGAGCGGTGAGCAGCCATGAGTGCAGCGGCATTTCTTGAGCACGCGCGGGAGTTCCTGCCGATCCTGCTGCAGGGG
GCGGTGGTCACGGTGGAGGT

Product: amino acid-binding periplasmic (PBP) ABC transporter protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSFVSFSRRAALVLCMLGSASLAWAQGGGAPTYNVGATATGVPFTFLDVKSNSIQGMMVDTVTAVGKAGGFSVNVQQTVF
AALIPSLTSSKIDIISAAMLKTPARAQVVDFSDPVYSYGEGLRVKADDAKTYASLDDLKGEVVGAQVGTVFLDMLNKKGI
FKEVRSYDSVADMTRDLALGRIKAGLGDQPILAYQIRQNTFQGVKLVSSYKPVNVGDVCLVVRKGDAELLGRINKAIAKI
KADGTLAAIVQKWGI

Sequences:

>Translated_255_residues
MSFVSFSRRAALVLCMLGSASLAWAQGGGAPTYNVGATATGVPFTFLDVKSNSIQGMMVDTVTAVGKAGGFSVNVQQTVF
AALIPSLTSSKIDIISAAMLKTPARAQVVDFSDPVYSYGEGLRVKADDAKTYASLDDLKGEVVGAQVGTVFLDMLNKKGI
FKEVRSYDSVADMTRDLALGRIKAGLGDQPILAYQIRQNTFQGVKLVSSYKPVNVGDVCLVVRKGDAELLGRINKAIAKI
KADGTLAAIVQKWGI
>Mature_254_residues
SFVSFSRRAALVLCMLGSASLAWAQGGGAPTYNVGATATGVPFTFLDVKSNSIQGMMVDTVTAVGKAGGFSVNVQQTVFA
ALIPSLTSSKIDIISAAMLKTPARAQVVDFSDPVYSYGEGLRVKADDAKTYASLDDLKGEVVGAQVGTVFLDMLNKKGIF
KEVRSYDSVADMTRDLALGRIKAGLGDQPILAYQIRQNTFQGVKLVSSYKPVNVGDVCLVVRKGDAELLGRINKAIAKIK
ADGTLAAIVQKWGI

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=221, Percent_Identity=32.1266968325792, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=30, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=30.1801801801802, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787031, Length=212, Percent_Identity=27.3584905660377, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=31.0344827586207, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=30.46875, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26937; Mature: 26805

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVSFSRRAALVLCMLGSASLAWAQGGGAPTYNVGATATGVPFTFLDVKSNSIQGMMVD
CCCCHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEHH
TVTAVGKAGGFSVNVQQTVFAALIPSLTSSKIDIISAAMLKTPARAQVVDFSDPVYSYGE
HHHHHCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHCCC
GLRVKADDAKTYASLDDLKGEVVGAQVGTVFLDMLNKKGIFKEVRSYDSVADMTRDLALG
CCEEEECCCHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RIKAGLGDQPILAYQIRQNTFQGVKLVSSYKPVNVGDVCLVVRKGDAELLGRINKAIAKI
HHHCCCCCCCEEEEEEHHCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KADGTLAAIVQKWGI
CCCCHHHHHHHHHCC
>Mature Secondary Structure 
SFVSFSRRAALVLCMLGSASLAWAQGGGAPTYNVGATATGVPFTFLDVKSNSIQGMMVD
CCCHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEHH
TVTAVGKAGGFSVNVQQTVFAALIPSLTSSKIDIISAAMLKTPARAQVVDFSDPVYSYGE
HHHHHCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHCCC
GLRVKADDAKTYASLDDLKGEVVGAQVGTVFLDMLNKKGIFKEVRSYDSVADMTRDLALG
CCEEEECCCHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RIKAGLGDQPILAYQIRQNTFQGVKLVSSYKPVNVGDVCLVVRKGDAELLGRINKAIAKI
HHHCCCCCCCEEEEEEHHCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
KADGTLAAIVQKWGI
CCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]