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The map label for this gene is mscL [H]

Identifier: 17547649

GI number: 17547649

Start: 3160747

End: 3161172

Strand: Direct

Name: mscL [H]

Synonym: RSc2930

Alternate gene names: 17547649

Gene position: 3160747-3161172 (Clockwise)

Preceding gene: 17547643

Following gene: 17547651

Centisome position: 85.05

GC content: 58.92

Gene sequence:

>426_bases
ATGGCACTAATGCAAGATTTCAAGAAATTCGCAATGCGCGGCAACGTGATCGACTTGGCGGTCGGTGTGATCATCGGTGC
GGCGTTTGGCAAGATCGTCGACTCGCTGGTCAACGACCTGATCATGCCGCTGGTTGCGCGCATCGTCGGCAAGCTCGATT
TCTCGAATCTGTTCATTCAACTGGCCGATGCACCCGCGGGCGTGCCACAGACGCTGGCCGACCTGAAGAAAGCGGGCGTG
CCGGTCTTCGCCTACGGCAACTTCATCACGGTCGCCGTCAACTTCCTGATCCTGGCCTTCATCGTGTTCCTGATGGTGCG
CGCGATCACCCGCGTGATCGACACCAACCCGCCGCCGGCCGACACGCCGGAAAACACGCTGCTGCTGCGCGATATCCGCG
ACAGCCTGAAATCCAAGAATCAATAA

Upstream 100 bases:

>100_bases
CGGCCCCGGCACGCCTCTTGCAACCTCGCTTTTAACAATTCAGGTCAACCGATCAGCAGCTTGGCCGTTTGTCCCGAATC
AAACAATCCGGAGAAAACCG

Downstream 100 bases:

>100_bases
AGGTAACCTTGAATCACACCGGATTCGGAATATCGATGAAGGTGTGGCGCACCCCGAAGTGCTCGGCAAGGTGGTTACCG
AGCGCGCGCACGCCGCCGCG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 140

Protein sequence:

>141_residues
MALMQDFKKFAMRGNVIDLAVGVIIGAAFGKIVDSLVNDLIMPLVARIVGKLDFSNLFIQLADAPAGVPQTLADLKKAGV
PVFAYGNFITVAVNFLILAFIVFLMVRAITRVIDTNPPPADTPENTLLLRDIRDSLKSKNQ

Sequences:

>Translated_141_residues
MALMQDFKKFAMRGNVIDLAVGVIIGAAFGKIVDSLVNDLIMPLVARIVGKLDFSNLFIQLADAPAGVPQTLADLKKAGV
PVFAYGNFITVAVNFLILAFIVFLMVRAITRVIDTNPPPADTPENTLLLRDIRDSLKSKNQ
>Mature_140_residues
ALMQDFKKFAMRGNVIDLAVGVIIGAAFGKIVDSLVNDLIMPLVARIVGKLDFSNLFIQLADAPAGVPQTLADLKKAGVP
VFAYGNFITVAVNFLILAFIVFLMVRAITRVIDTNPPPADTPENTLLLRDIRDSLKSKNQ

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=143, Percent_Identity=43.3566433566434, Blast_Score=113, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15299; Mature: 15168

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALMQDFKKFAMRGNVIDLAVGVIIGAAFGKIVDSLVNDLIMPLVARIVGKLDFSNLFIQ
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LADAPAGVPQTLADLKKAGVPVFAYGNFITVAVNFLILAFIVFLMVRAITRVIDTNPPPA
HCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DTPENTLLLRDIRDSLKSKNQ
CCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALMQDFKKFAMRGNVIDLAVGVIIGAAFGKIVDSLVNDLIMPLVARIVGKLDFSNLFIQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LADAPAGVPQTLADLKKAGVPVFAYGNFITVAVNFLILAFIVFLMVRAITRVIDTNPPPA
HCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DTPENTLLLRDIRDSLKSKNQ
CCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA