BacMap

Definition	Ralstonia solanacearum GMI1000, complete genome.
Accession	NC_003295
Length	3,716,413

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The map label for this gene is paaG1 [H]

Identifier: 17547592

GI number: 17547592

Start: 3102492

End: 3103280

Strand: Direct

Name: paaG1 [H]

Synonym: RSc2873

Alternate gene names: 17547592

Gene position: 3102492-3103280 (Clockwise)

Preceding gene: 17547590

Following gene: 17547593

Centisome position: 83.48

GC content: 68.44

Gene sequence:

>789_bases
ATGACCAGCCCGACCATTCTGCTCGACTGGCATGATCGCGTGGCCACCATCACGCTGAACCGTCCCGACCAGCTCAACAG
CTTCACCGCCGGCATGCACCGGGAACTGCGCGAGGCCTTGGACAAGGTGGAGCGGCAGCGGGCTCGCGCACTGATCCTGA
CCGGCGCCGGGCGTGGCTTCTGCGCGGGGCAGGATCTCTCCGAGCTCAATGTGACACCCGGGCAGATGACGGACCTGGGT
ACGCTCATCGACCAGCAGTTCAACCCGCTGGTGCGCCGACTGCGCGCCCTGCCGCTGCCTGTGATTGCCGCGGTCAACGG
CGTGGCGGCCGGCGCGGGTGCCAACCTGGCGCTGGCCTGCGACATCGTGCTGGCCGCGGAAAACGCCTGCTTCCTGCAAG
CCTTCGTCAAGATCGGGTTGCTGCCGGACTCGGGCGGCACGTGGTTCATGCCGCAGCGCATCGGCGTGGCGCGGGCAATG
GGGCTGGCGATGCTGGGCGAGAAGCTCGACGCCAGGACCGCGCTGGCCTGGGGGCTGATCTGGGGCGTCTATCACGATGC
CAAGATCCTGATGACCGAGGTGCAGGCGATGGCCGACCACCTGTCCAAGCAGCCCACTCGCGCGCTGGCCGCCATCAAGC
GCGCCATGCATGCGGCGCCGACGCAGTCGCTCGACGCCCAGCTCGACCTGGAGCGCGACCTTCAGCGCGAGCTCGGCCAT
TCGCGTGACTACGCCGAGGGGGTCGATGCGTTCCTCCACAAGCGCGTGGCCCACTTCACCGGCGAATGA

Upstream 100 bases:

>100_bases
CAGAATGTGTTGGTAGGACATGGGAACTCCAGTTGGAATCGATGTAATATTAGCCGACCAACCGGTCGGTTAATCAACCT
GCGACCGAGGAGGAGACAAG

Downstream 100 bases:

>100_bases
CCGTGGCCGAAACCGCCTTCCCCGACGCGCAGGCGCTGGCCGAAGCCGCCTGCCGAAGCATGCTCGACGCCGATGCCTGC
TCCCGCTGGCTCGGCATGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MTSPTILLDWHDRVATITLNRPDQLNSFTAGMHRELREALDKVERQRARALILTGAGRGFCAGQDLSELNVTPGQMTDLG
TLIDQQFNPLVRRLRALPLPVIAAVNGVAAGAGANLALACDIVLAAENACFLQAFVKIGLLPDSGGTWFMPQRIGVARAM
GLAMLGEKLDARTALAWGLIWGVYHDAKILMTEVQAMADHLSKQPTRALAAIKRAMHAAPTQSLDAQLDLERDLQRELGH
SRDYAEGVDAFLHKRVAHFTGE

Sequences:

>Translated_262_residues
MTSPTILLDWHDRVATITLNRPDQLNSFTAGMHRELREALDKVERQRARALILTGAGRGFCAGQDLSELNVTPGQMTDLG
TLIDQQFNPLVRRLRALPLPVIAAVNGVAAGAGANLALACDIVLAAENACFLQAFVKIGLLPDSGGTWFMPQRIGVARAM
GLAMLGEKLDARTALAWGLIWGVYHDAKILMTEVQAMADHLSKQPTRALAAIKRAMHAAPTQSLDAQLDLERDLQRELGH
SRDYAEGVDAFLHKRVAHFTGE
>Mature_261_residues
TSPTILLDWHDRVATITLNRPDQLNSFTAGMHRELREALDKVERQRARALILTGAGRGFCAGQDLSELNVTPGQMTDLGT
LIDQQFNPLVRRLRALPLPVIAAVNGVAAGAGANLALACDIVLAAENACFLQAFVKIGLLPDSGGTWFMPQRIGVARAMG
LAMLGEKLDARTALAWGLIWGVYHDAKILMTEVQAMADHLSKQPTRALAAIKRAMHAAPTQSLDAQLDLERDLQRELGHS
RDYAEGVDAFLHKRVAHFTGE

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=32.8, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=28.4584980237154, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI45643119, Length=223, Percent_Identity=27.3542600896861, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI260275230, Length=223, Percent_Identity=27.3542600896861, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI260274832, Length=223, Percent_Identity=27.3542600896861, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=29.1497975708502, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI157694516, Length=224, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI213417737, Length=224, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI116235487, Length=177, Percent_Identity=31.0734463276836, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI37594471, Length=234, Percent_Identity=26.0683760683761, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594469, Length=234, Percent_Identity=26.0683760683761, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=29.1044776119403, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4757968, Length=193, Percent_Identity=27.4611398963731, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI49355787, Length=193, Percent_Identity=27.4611398963731, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI25453481, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51538573, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51538581, Length=182, Percent_Identity=28.021978021978, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI68989263, Length=215, Percent_Identity=29.7674418604651, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI221307494, Length=163, Percent_Identity=30.0613496932515, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI221136756, Length=265, Percent_Identity=25.6603773584906, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI221136753, Length=265, Percent_Identity=25.6603773584906, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI20127408, Length=173, Percent_Identity=27.1676300578035, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI157694520, Length=206, Percent_Identity=28.1553398058252, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=58.1673306772908, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=33.4630350194552, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=31.9391634980989, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=31.2977099236641, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=27.4509803921569, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=31.25, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788682, Length=212, Percent_Identity=30.6603773584906, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=35.2, Blast_Score=138, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17554946, Length=243, Percent_Identity=31.6872427983539, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17549921, Length=241, Percent_Identity=29.045643153527, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=264, Percent_Identity=27.6515151515151, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=247, Percent_Identity=25.5060728744939, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=245, Percent_Identity=29.3877551020408, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=231, Percent_Identity=26.8398268398268, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=170, Percent_Identity=32.9411764705882, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=243, Percent_Identity=23.4567901234568, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=31.4741035856574, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=31.4741035856574, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653139, Length=273, Percent_Identity=28.9377289377289, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922422, Length=267, Percent_Identity=28.4644194756554, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=28.5140562248996, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24654903, Length=255, Percent_Identity=23.921568627451, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28571729, Length=249, Percent_Identity=24.8995983935743, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=249, Percent_Identity=24.8995983935743, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=218, Percent_Identity=25.2293577981651, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24650670, Length=179, Percent_Identity=28.4916201117318, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28490; Mature: 28359

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPTILLDWHDRVATITLNRPDQLNSFTAGMHRELREALDKVERQRARALILTGAGRGF
CCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCC
CAGQDLSELNVTPGQMTDLGTLIDQQFNPLVRRLRALPLPVIAAVNGVAAGAGANLALAC
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEE
DIVLAAENACFLQAFVKIGLLPDSGGTWFMPQRIGVARAMGLAMLGEKLDARTALAWGLI
EEEHCCCCHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGVYHDAKILMTEVQAMADHLSKQPTRALAAIKRAMHAAPTQSLDAQLDLERDLQRELGH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCC
SRDYAEGVDAFLHKRVAHFTGE
CCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSPTILLDWHDRVATITLNRPDQLNSFTAGMHRELREALDKVERQRARALILTGAGRGF
CCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCC
CAGQDLSELNVTPGQMTDLGTLIDQQFNPLVRRLRALPLPVIAAVNGVAAGAGANLALAC
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEE
DIVLAAENACFLQAFVKIGLLPDSGGTWFMPQRIGVARAMGLAMLGEKLDARTALAWGLI
EEEHCCCCHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGVYHDAKILMTEVQAMADHLSKQPTRALAAIKRAMHAAPTQSLDAQLDLERDLQRELGH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCC
SRDYAEGVDAFLHKRVAHFTGE
CCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]