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The map label for this gene is paaI [H]

Identifier: 17547593

GI number: 17547593

Start: 3103277

End: 3103744

Strand: Direct

Name: paaI [H]

Synonym: RSc2874

Alternate gene names: 17547593

Gene position: 3103277-3103744 (Clockwise)

Preceding gene: 17547592

Following gene: 17547594

Centisome position: 83.5

GC content: 70.09

Gene sequence:

>468_bases
ATGACCGTGGCCGAAACCGCCTTCCCCGACGCGCAGGCGCTGGCCGAAGCCGCCTGCCGAAGCATGCTCGACGCCGATGC
CTGCTCCCGCTGGCTCGGCATGCGCGTGGAAGCGGTCGGCCCCGGCTACGCGCGCCTGGCCATGGCGGTGCGGCCCGAAT
TCCTCAACGGGCACCGCACCTGCCATGGCGGGCTGATTTTCACGCTGGCCGATTCGGCCTTCGCGTTTGCCTGCAACAGC
CACAACCACAACACGGTCGCGGCGGGCTGCAGCGTCGAATTCCTGCGGCCGGCGCACGACGGCGACGTGCTGACTGCCGC
AGCCGAGGAGCAAACCCTGTCCGGCCGCCACGGGATCTACGACGTGCGCGTGACCAACCAGGCCGGCGACGTGGTCGCCA
TGTTCCGGGGGAAATCGGCGCAGATCCGAGGCCACGTCATCCCCGTTCCGGACAGCGGCGACCGCTGA

Upstream 100 bases:

>100_bases
ACCTGGAGCGCGACCTTCAGCGCGAGCTCGGCCATTCGCGTGACTACGCCGAGGGGGTCGATGCGTTCCTCCACAAGCGC
GTGGCCCACTTCACCGGCGA

Downstream 100 bases:

>100_bases
TCCCCTCCTATATATAGAGAGTTGCCACGACATGGGCCCGCCCGATGGTGCCCAAGGAGACCTGCATGCGCCCCCGCTTG
ACCGACCTGCCGCTCGATCC

Product: phenylacetic acid degradation protein

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MTVAETAFPDAQALAEAACRSMLDADACSRWLGMRVEAVGPGYARLAMAVRPEFLNGHRTCHGGLIFTLADSAFAFACNS
HNHNTVAAGCSVEFLRPAHDGDVLTAAAEEQTLSGRHGIYDVRVTNQAGDVVAMFRGKSAQIRGHVIPVPDSGDR

Sequences:

>Translated_155_residues
MTVAETAFPDAQALAEAACRSMLDADACSRWLGMRVEAVGPGYARLAMAVRPEFLNGHRTCHGGLIFTLADSAFAFACNS
HNHNTVAAGCSVEFLRPAHDGDVLTAAAEEQTLSGRHGIYDVRVTNQAGDVVAMFRGKSAQIRGHVIPVPDSGDR
>Mature_154_residues
TVAETAFPDAQALAEAACRSMLDADACSRWLGMRVEAVGPGYARLAMAVRPEFLNGHRTCHGGLIFTLADSAFAFACNSH
NHNTVAAGCSVEFLRPAHDGDVLTAAAEEQTLSGRHGIYDVRVTNQAGDVVAMFRGKSAQIRGHVIPVPDSGDR

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=126, Percent_Identity=44.4444444444444, Blast_Score=126, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 16441; Mature: 16309

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAETAFPDAQALAEAACRSMLDADACSRWLGMRVEAVGPGYARLAMAVRPEFLNGHRT
CCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCEEEECCCCHHHHHHHHCHHHHCCCCE
CHGGLIFTLADSAFAFACNSHNHNTVAAGCSVEFLRPAHDGDVLTAAAEEQTLSGRHGIY
ECCCEEEEEECCEEEEEECCCCCCEEEECCEEEEECCCCCCCEEEECCCCCCCCCCCCEE
DVRVTNQAGDVVAMFRGKSAQIRGHVIPVPDSGDR
EEEEECCCCCEEEEEECCCCEECCCEEECCCCCCC
>Mature Secondary Structure 
TVAETAFPDAQALAEAACRSMLDADACSRWLGMRVEAVGPGYARLAMAVRPEFLNGHRT
CCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCEEEECCCCHHHHHHHHCHHHHCCCCE
CHGGLIFTLADSAFAFACNSHNHNTVAAGCSVEFLRPAHDGDVLTAAAEEQTLSGRHGIY
ECCCEEEEEECCEEEEEECCCCCCEEEECCEEEEECCCCCCCEEEECCCCCCCCCCCCEE
DVRVTNQAGDVVAMFRGKSAQIRGHVIPVPDSGDR
EEEEECCCCCEEEEEECCCCEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]