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The map label for this gene is proS [H]

Identifier: 17547535

GI number: 17547535

Start: 3029055

End: 3030779

Strand: Reverse

Name: proS [H]

Synonym: RSc2816

Alternate gene names: 17547535

Gene position: 3030779-3029055 (Counterclockwise)

Preceding gene: 17547538

Following gene: 17547534

Centisome position: 81.55

GC content: 67.07

Gene sequence:

>1725_bases
ATGAAAGCGTCCCAGTTCTTCATTTCCACGCTCAAGGAAGCCCCCGCCGACGCGGAGATCGTCTCGCACAAGCTGATGAT
GCGTGCCGGCATGATCAAGAAGCTTGGCGCGGGCCTCTATACGTATATGCCCGTGGGTCTGCGCGTGATCCGCAAGGTCG
AGCAGATCGTGCGTGAGGAAATGAACGCCAGTGGTGCGGTGGAAGTGCTGATGCCCGTGGTGCAGCCCGCCGAGCTGTGG
CAGGAGACCGGCCGCTGGGACAAGATGGGCGACGAGATGATGCGCGTGAAAGATCGCCACGAGCGCGATCTTGTCATCCA
GCCGACCTCCGAAGAGGTGGTGACCGACATCGCCCGCACCGAGATCCGCTCGTACAAGCAGATGCCGGTCAACTTCTACC
AGATCCAGACCAAGTTTCGCGATGAGCGCCGTCCTCGTTTCGGCATCATGCGCGGCCGCGAGTTCACCATGAAGGACGCG
TACTCCTTCGACCGCGACGCCGAGGGTCTGAAGGTCTCGTACGAGAAGATGTACGGCGCCTACACGCGCATCTTCCAGCG
CTTCGGGCTGGAGTTCCGTGCGGTGGCGGCCGACAACGGCGCCATCGGCGGCTCGGGCTCGCATGAGTTCCACGTGATCG
CCGACACCGGCGAGGACGCCATCGTCTACAGCCCGGACTCGGACTACGCCGCCAACATCGAGGCTGCCGAGGCGCTGGCC
CCGGCCATGCCGCGCGGCGCTGCCACCGAGGCGCTCACCAAGACGCACACCCCCAAGCGCGCCAAGTGCGAGGCCGTGGC
CGAGCAGCTGGGCATTGCGCTGCAGCGCACGGTCAAATCCATCGTGCTGGCCAAGGACGTCGAAGGCGGCGAGCCGCAGA
TCTGGCTGCTGCTGCTGCGCGGCGACCACGAACTGAATGAGATCAAGGCCTCGAAGGTGCCGGGCCTGGCCGATTTCCGC
TTTGCCACCGAGGGCGAGATCCTGCGCGCGTTCGGCACGCCGCCGGGCTACCTGGGCCCGATCGGCACCAAGCAGCCGGT
CAAGGTGGTGGCCGACCGCACCGTCGCCGCCATGAGCGACTTCGTCGTCGGCGCCAACGAGGAGGATTTCCACTACACCG
GCGTCAACTGGGGCCGCGACCTGCCCGAGGCCGAAGTCTACGACCTGCGCAATGTGGTGGAGGGCGATCCCTCGCCGGAC
GGCAAGGGCACGCTGGCGATCTGCCGCGGCATCGAAGTCGGCCACGTGTTCATGCTGGGCACGCGCTACTCTGAATCGAT
GAGCGCCACCTTCCTCGACGAGAACGGCAAGACCCAGCCGATGATGATGGGCTGCTACGGCATCGGCATCACGCGGATCC
TGGGCGCGGCCATCGAGCAGAACTACGATGCGCGCGGCATCATCTGGCCGGTGTCGATCGCGCCGTTTGAGGTGGTGATC
TGCCCGGTGGGCTACGACCGTTCCGAGGCCGTGCGCGCCGAGGCCGACCGCCTGCACGCGGAACTGGCCGCCGCCGGCAT
CGACGTGATCCTGGACGACCGCGGCGAGCGCCCCGGCGCGATGTTCGCCGACTGGGAGCTGATCGGCGTGCCGTTCCGCG
TGGTGGTGGGCGAGCGCGGCCTGAAGGACGGCAAGCTCGAACTGCAGGGCCGCCGCGACGAGGCCGCCGCCGCCGTGGCG
CCGGCCGACGTGCTGGCCACCCTCAAGTCGCGCCTGGCGCAATAA

Upstream 100 bases:

>100_bases
CGATGTGCGCTTCGCATCCCGCCGTATCTCCTGCTTCCGGTGGCTTTCGCCTTTATCCTCGCGGCTTTGGTTGCCGCGGC
TATCCCTTATTGTCAGTCGC

Downstream 100 bases:

>100_bases
CGGTGCCGGACGCCATGCGCCGCTTCCCGGTCCGCCTGATCGCCGCCACGCTGTGCGCGGTGGCGCTGCTCGCGGGTACG
CGCTCGGCGTGGGCGGGTGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREEMNASGAVEVLMPVVQPAELW
QETGRWDKMGDEMMRVKDRHERDLVIQPTSEEVVTDIARTEIRSYKQMPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKVSYEKMYGAYTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYSPDSDYAANIEAAEALA
PAMPRGAATEALTKTHTPKRAKCEAVAEQLGIALQRTVKSIVLAKDVEGGEPQIWLLLLRGDHELNEIKASKVPGLADFR
FATEGEILRAFGTPPGYLGPIGTKQPVKVVADRTVAAMSDFVVGANEEDFHYTGVNWGRDLPEAEVYDLRNVVEGDPSPD
GKGTLAICRGIEVGHVFMLGTRYSESMSATFLDENGKTQPMMMGCYGIGITRILGAAIEQNYDARGIIWPVSIAPFEVVI
CPVGYDRSEAVRAEADRLHAELAAAGIDVILDDRGERPGAMFADWELIGVPFRVVVGERGLKDGKLELQGRRDEAAAAVA
PADVLATLKSRLAQ

Sequences:

>Translated_574_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREEMNASGAVEVLMPVVQPAELW
QETGRWDKMGDEMMRVKDRHERDLVIQPTSEEVVTDIARTEIRSYKQMPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKVSYEKMYGAYTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYSPDSDYAANIEAAEALA
PAMPRGAATEALTKTHTPKRAKCEAVAEQLGIALQRTVKSIVLAKDVEGGEPQIWLLLLRGDHELNEIKASKVPGLADFR
FATEGEILRAFGTPPGYLGPIGTKQPVKVVADRTVAAMSDFVVGANEEDFHYTGVNWGRDLPEAEVYDLRNVVEGDPSPD
GKGTLAICRGIEVGHVFMLGTRYSESMSATFLDENGKTQPMMMGCYGIGITRILGAAIEQNYDARGIIWPVSIAPFEVVI
CPVGYDRSEAVRAEADRLHAELAAAGIDVILDDRGERPGAMFADWELIGVPFRVVVGERGLKDGKLELQGRRDEAAAAVA
PADVLATLKSRLAQ
>Mature_574_residues
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREEMNASGAVEVLMPVVQPAELW
QETGRWDKMGDEMMRVKDRHERDLVIQPTSEEVVTDIARTEIRSYKQMPVNFYQIQTKFRDERRPRFGIMRGREFTMKDA
YSFDRDAEGLKVSYEKMYGAYTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYSPDSDYAANIEAAEALA
PAMPRGAATEALTKTHTPKRAKCEAVAEQLGIALQRTVKSIVLAKDVEGGEPQIWLLLLRGDHELNEIKASKVPGLADFR
FATEGEILRAFGTPPGYLGPIGTKQPVKVVADRTVAAMSDFVVGANEEDFHYTGVNWGRDLPEAEVYDLRNVVEGDPSPD
GKGTLAICRGIEVGHVFMLGTRYSESMSATFLDENGKTQPMMMGCYGIGITRILGAAIEQNYDARGIIWPVSIAPFEVVI
CPVGYDRSEAVRAEADRLHAELAAAGIDVILDDRGERPGAMFADWELIGVPFRVVVGERGLKDGKLELQGRRDEAAAAVA
PADVLATLKSRLAQ

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=249, Percent_Identity=41.7670682730924, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1786392, Length=574, Percent_Identity=56.9686411149826, Blast_Score=682, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=274, Percent_Identity=35.4014598540146, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=87, Percent_Identity=37.9310344827586, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=548, Percent_Identity=29.9270072992701, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24656200, Length=247, Percent_Identity=36.8421052631579, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63244; Mature: 63244

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNASGAVEVLMPVVQPAELWQETGRWDKMGDEMMRVKDRHERDLVIQPTSEEVVTDIART
CCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EIRSYKQMPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDAEGLKVSYEKMYGA
HHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEHHHHHHHH
YTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYSPDSDYAANIEAAEALA
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCHHHHHHC
PAMPRGAATEALTKTHTPKRAKCEAVAEQLGIALQRTVKSIVLAKDVEGGEPQIWLLLLR
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
GDHELNEIKASKVPGLADFRFATEGEILRAFGTPPGYLGPIGTKQPVKVVADRTVAAMSD
CCCHHHHHHHHCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FVVGANEEDFHYTGVNWGRDLPEAEVYDLRNVVEGDPSPDGKGTLAICRGIEVGHVFMLG
HEECCCCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEE
TRYSESMSATFLDENGKTQPMMMGCYGIGITRILGAAIEQNYDARGIIWPVSIAPFEVVI
CCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEE
CPVGYDRSEAVRAEADRLHAELAAAGIDVILDDRGERPGAMFADWELIGVPFRVVVGERG
EECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECEEEECCCEEEEECCCC
LKDGKLELQGRRDEAAAAVAPADVLATLKSRLAQ
CCCCCEEECCCCCCHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREE
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MNASGAVEVLMPVVQPAELWQETGRWDKMGDEMMRVKDRHERDLVIQPTSEEVVTDIART
CCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EIRSYKQMPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDAEGLKVSYEKMYGA
HHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEHHHHHHHH
YTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYSPDSDYAANIEAAEALA
HHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCHHHHHHC
PAMPRGAATEALTKTHTPKRAKCEAVAEQLGIALQRTVKSIVLAKDVEGGEPQIWLLLLR
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
GDHELNEIKASKVPGLADFRFATEGEILRAFGTPPGYLGPIGTKQPVKVVADRTVAAMSD
CCCHHHHHHHHCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FVVGANEEDFHYTGVNWGRDLPEAEVYDLRNVVEGDPSPDGKGTLAICRGIEVGHVFMLG
HEECCCCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEE
TRYSESMSATFLDENGKTQPMMMGCYGIGITRILGAAIEQNYDARGIIWPVSIAPFEVVI
CCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEE
CPVGYDRSEAVRAEADRLHAELAAAGIDVILDDRGERPGAMFADWELIGVPFRVVVGERG
EECCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECEEEECCCEEEEECCCC
LKDGKLELQGRRDEAAAAVAPADVLATLKSRLAQ
CCCCCEEECCCCCCHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA