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The map label for this gene is proB [H]

Identifier: 17547538

GI number: 17547538

Start: 3032406

End: 3033530

Strand: Reverse

Name: proB [H]

Synonym: RSc2819

Alternate gene names: 17547538

Gene position: 3033530-3032406 (Counterclockwise)

Preceding gene: 17547539

Following gene: 17547535

Centisome position: 81.63

GC content: 70.22

Gene sequence:

>1125_bases
ATGGCCCTGCGTTCGCTGATCGCCGATGCGCGCCGCCTCGTCGTCAAGGTCGGTTCCAGCCTGGTCACCGATGACGGCCG
CGGCCTCGACCAGGCCGCCATCGCCCGCTGGGCCGCGCAGATCGCGGCGCTGCGCGCGGCCGGCAAGGAGGTCGTGCTGG
TGAGCTCCGGCGCCATCGCCGAAGGCATGCAGCGGCTGGGCTGGACCCGGCGGCCGAAGGAGATCCACGAGCTGCAGGCC
GCTGCCGCCGTCGGACAGATGGGCCTGGCCCAGGTGTACGAATCGGAGTTCGCGCGGCACGGTATCCGCACGGCGCAGGT
GCTGCTGACGCATGGCGATCTGGCTGACCGCGAGCGCTACCTGAACGCGCGCTCGACGCTGCTGACCCTGCTGGGCCTCG
GCGTGGTGCCGATCATCAACGAGAACGACACGGTCGTCACCGACGAGATCAAGTTCGGCGACAACGATACGCTGGGCGCG
CTGGTCACCAACCTGATCGAAGGCGATGCGCTGATCATCCTGACGGATCAGCGTGGCCTGTACACCGCCGATCCGCGCAA
GCACCCCGATGCGCGCTTCGTCGACGAAGCCCAGGCCGGGGCGCCGGAGCTGGAGGCCATGGCTGGCGGTGCCGGCAGCA
GCATCGGCAAGGGCGGCATGCTGACCAAGATCGTGGCGGCCAAGCGGGCAGCCAAGTCGGGGGCCCACACCGTGATCGCC
TCGGGCCGCGAGGCCGACGTGCTGGCGCGGCTGGCCGGCGGCGAGGCCATCGGCACGCAACTGCGCGCGCCGACCGGGCG
CATGGCTGCGCGCAAGCAGTGGATGATCGACCACCTGCAACTGCGCGGCCGCGTCGTGCTGGATGCCGGTGCGGTCGACA
AGCTCACGGCCGGCGGCAAGTCGCTGTTGCCGATCGGCGTGACGGAAGTGCAGGGCGAGTTTGCGCGCGGGGAAGTGATT
TCGTGCGTCGACACGGCCGGGCGCGAAGTGGCGCGCGGGCTGACCAACTATTCCGCCGCCGAGGCGCGGCTGATTGCCCG
CAAAGCGTCTTCGGAGATCGAGGCGGTGCTGGGATATGTCAGCGCCGCCGAGCTGGTGCACCGCGACAACCTGGTGCTGC
TCTGA

Upstream 100 bases:

>100_bases
GCGCGCCTCGACCCGACGCTGCACAACGTCGACCACGACGGCAGCGCCAACGCATAACACCGCAGCATCACAACACTTCG
TCTCCGGAGACTCGCCCACC

Downstream 100 bases:

>100_bases
ACCTTTTTCGGGATGGGCCGCCCGGTCCATCCCGGGCGGCTGATCTTCTTACGGCCCGTACATGCGCCCGGACGAGGCCG
TGCCCAGCCGCCGCACGAGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MALRSLIADARRLVVKVGSSLVTDDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIAEGMQRLGWTRRPKEIHELQA
AAAVGQMGLAQVYESEFARHGIRTAQVLLTHGDLADRERYLNARSTLLTLLGLGVVPIINENDTVVTDEIKFGDNDTLGA
LVTNLIEGDALIILTDQRGLYTADPRKHPDARFVDEAQAGAPELEAMAGGAGSSIGKGGMLTKIVAAKRAAKSGAHTVIA
SGREADVLARLAGGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVDKLTAGGKSLLPIGVTEVQGEFARGEVI
SCVDTAGREVARGLTNYSAAEARLIARKASSEIEAVLGYVSAAELVHRDNLVLL

Sequences:

>Translated_374_residues
MALRSLIADARRLVVKVGSSLVTDDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIAEGMQRLGWTRRPKEIHELQA
AAAVGQMGLAQVYESEFARHGIRTAQVLLTHGDLADRERYLNARSTLLTLLGLGVVPIINENDTVVTDEIKFGDNDTLGA
LVTNLIEGDALIILTDQRGLYTADPRKHPDARFVDEAQAGAPELEAMAGGAGSSIGKGGMLTKIVAAKRAAKSGAHTVIA
SGREADVLARLAGGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVDKLTAGGKSLLPIGVTEVQGEFARGEVI
SCVDTAGREVARGLTNYSAAEARLIARKASSEIEAVLGYVSAAELVHRDNLVLL
>Mature_373_residues
ALRSLIADARRLVVKVGSSLVTDDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIAEGMQRLGWTRRPKEIHELQAA
AAVGQMGLAQVYESEFARHGIRTAQVLLTHGDLADRERYLNARSTLLTLLGLGVVPIINENDTVVTDEIKFGDNDTLGAL
VTNLIEGDALIILTDQRGLYTADPRKHPDARFVDEAQAGAPELEAMAGGAGSSIGKGGMLTKIVAAKRAAKSGAHTVIAS
GREADVLARLAGGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVDKLTAGGKSLLPIGVTEVQGEFARGEVIS
CVDTAGREVARGLTNYSAAEARLIARKASSEIEAVLGYVSAAELVHRDNLVLL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=34.2465753424658, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=34.0136054421769, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.4426229508197, Blast_Score=285, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17569969, Length=277, Percent_Identity=34.6570397111913, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=277, Percent_Identity=34.6570397111913, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=418, Percent_Identity=35.8851674641148, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6321822, Length=425, Percent_Identity=31.7647058823529, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21357643, Length=288, Percent_Identity=34.7222222222222, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39367; Mature: 39235

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRSLIADARRLVVKVGSSLVTDDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIA
CCHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHH
EGMQRLGWTRRPKEIHELQAAAAVGQMGLAQVYESEFARHGIRTAQVLLTHGDLADRERY
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
LNARSTLLTLLGLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALIILTDQRGL
HHHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCC
YTADPRKHPDARFVDEAQAGAPELEAMAGGAGSSIGKGGMLTKIVAAKRAAKSGAHTVIA
EECCCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SGREADVLARLAGGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVDKLTAGGK
CCCCHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHCCCCC
SLLPIGVTEVQGEFARGEVISCVDTAGREVARGLTNYSAAEARLIARKASSEIEAVLGYV
CCCCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SAAELVHRDNLVLL
HHHHHHHCCCEEEC
>Mature Secondary Structure 
ALRSLIADARRLVVKVGSSLVTDDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIA
CHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHH
EGMQRLGWTRRPKEIHELQAAAAVGQMGLAQVYESEFARHGIRTAQVLLTHGDLADRERY
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHH
LNARSTLLTLLGLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALIILTDQRGL
HHHHHHHHHHHHCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCC
YTADPRKHPDARFVDEAQAGAPELEAMAGGAGSSIGKGGMLTKIVAAKRAAKSGAHTVIA
EECCCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEE
SGREADVLARLAGGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVDKLTAGGK
CCCCHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHCCCCC
SLLPIGVTEVQGEFARGEVISCVDTAGREVARGLTNYSAAEARLIARKASSEIEAVLGYV
CCCCCCHHHHCCCHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
SAAELVHRDNLVLL
HHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA