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The map label for this gene is purN [H]

Identifier: 17547173

GI number: 17547173

Start: 2660390

End: 2661040

Strand: Reverse

Name: purN [H]

Synonym: RSc2454

Alternate gene names: 17547173

Gene position: 2661040-2660390 (Counterclockwise)

Preceding gene: 17547175

Following gene: 17547172

Centisome position: 71.6

GC content: 68.36

Gene sequence:

>651_bases
ATGAAAAATATCGTCATTCTCATTTCCGGACGCGGCTCGAATATGGAAGCCATCGTCCGCGCATGCCAGGCCGAGGGCTG
GCCGGGGCGCATCGCCGCCGTGATCTCCAACCGCCCGGACGCCGCCGGCCTCAGGTTTGCGGCTTCGCACGGCATCGCCA
CCGCCGTGGTCGACCACAAGGCCTTCCCCGACCGCGACAGCTTCGACACCGCCCTGGCCGCGGCCATCGACGGCTTCGCG
CCGGATCTGGTGGTGCTGGCGGGTTTCATGCGCATCCTGACGCCGGGCTTCGTGCAGCGCTACGCAGGACGCCTGCTCAA
TATCCATCCGTCGCTGCTGCCCTGCTTTCCCGGGCTGCATACGCACGAGCAGGCGCTGGCCATGGGCGTCAAGGTGCACG
GCGCAACGGTCCATTTCGTGACCGCCGAACTGGACCACGGCCCGATCGTGCTGCAGGCCGCCATTGAGGTGCGCGCCGGC
GATACGCCGGATTCGCTGGCGGCGCGCCTGCTGGAGCAGGAGCACGTCATCTATCCGCGCGCCGTGCGCTGGTTCGTCGA
AGGGCGGCTGCAGATGGAGCGCGGCGTGGTGCGCGTGTCGCCGGAAGCGTCGCAGCTCGTGCTCGGCGCCGATATGCGGG
AGGCCGTATGA

Upstream 100 bases:

>100_bases
CGGCTAAATACCACGCTCTGGACAACGCGAGGCGGGCGCCGCTGTGCGCGCGTCACATGGTTTTACCGGGGGAGCGCGGC
GCACTTGATAAAATGCTGCG

Downstream 100 bases:

>100_bases
GCGGGTCGGGTCGTCAACGTTCTCCGGGCGGTCGGGGCCCTGCGGCAGGTAAGCCCCGCGACGGGCGTCCTCAGCGTCCG
GCGGGTCGTGCTCCGCAGGG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKNIVILISGRGSNMEAIVRACQAEGWPGRIAAVISNRPDAAGLRFAASHGIATAVVDHKAFPDRDSFDTALAAAIDGFA
PDLVVLAGFMRILTPGFVQRYAGRLLNIHPSLLPCFPGLHTHEQALAMGVKVHGATVHFVTAELDHGPIVLQAAIEVRAG
DTPDSLAARLLEQEHVIYPRAVRWFVEGRLQMERGVVRVSPEASQLVLGADMREAV

Sequences:

>Translated_216_residues
MKNIVILISGRGSNMEAIVRACQAEGWPGRIAAVISNRPDAAGLRFAASHGIATAVVDHKAFPDRDSFDTALAAAIDGFA
PDLVVLAGFMRILTPGFVQRYAGRLLNIHPSLLPCFPGLHTHEQALAMGVKVHGATVHFVTAELDHGPIVLQAAIEVRAG
DTPDSLAARLLEQEHVIYPRAVRWFVEGRLQMERGVVRVSPEASQLVLGADMREAV
>Mature_216_residues
MKNIVILISGRGSNMEAIVRACQAEGWPGRIAAVISNRPDAAGLRFAASHGIATAVVDHKAFPDRDSFDTALAAAIDGFA
PDLVVLAGFMRILTPGFVQRYAGRLLNIHPSLLPCFPGLHTHEQALAMGVKVHGATVHFVTAELDHGPIVLQAAIEVRAG
DTPDSLAARLLEQEHVIYPRAVRWFVEGRLQMERGVVRVSPEASQLVLGADMREAV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=45.5026455026455, Blast_Score=173, Evalue=9e-44,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=45.5026455026455, Blast_Score=173, Evalue=9e-44,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=45.5026455026455, Blast_Score=173, Evalue=9e-44,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=56.4766839378238, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI1787483, Length=195, Percent_Identity=36.4102564102564, Blast_Score=109, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=41.8994413407821, Blast_Score=150, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6320616, Length=203, Percent_Identity=31.5270935960591, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24582400, Length=205, Percent_Identity=42.4390243902439, Blast_Score=160, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23222; Mature: 23222

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVILISGRGSNMEAIVRACQAEGWPGRIAAVISNRPDAAGLRFAASHGIATAVVDHK
CCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEECCC
AFPDRDSFDTALAAAIDGFAPDLVVLAGFMRILTPGFVQRYAGRLLNIHPSLLPCFPGLH
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCHHHCCCCCC
THEQALAMGVKVHGATVHFVTAELDHGPIVLQAAIEVRAGDTPDSLAARLLEQEHVIYPR
CHHHHHHHCEEECCEEEEEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCCHH
AVRWFVEGRLQMERGVVRVSPEASQLVLGADMREAV
HHHHHHHHHHHHHCCEEEECCCHHEEEECCCHHHCC
>Mature Secondary Structure
MKNIVILISGRGSNMEAIVRACQAEGWPGRIAAVISNRPDAAGLRFAASHGIATAVVDHK
CCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCCCEEEEECCC
AFPDRDSFDTALAAAIDGFAPDLVVLAGFMRILTPGFVQRYAGRLLNIHPSLLPCFPGLH
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCHHHCCCCCC
THEQALAMGVKVHGATVHFVTAELDHGPIVLQAAIEVRAGDTPDSLAARLLEQEHVIYPR
CHHHHHHHCEEECCEEEEEEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCCHH
AVRWFVEGRLQMERGVVRVSPEASQLVLGADMREAV
HHHHHHHHHHHHHCCEEEECCCHHEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]