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The map label for this gene is leuD

Identifier: 17546708

GI number: 17546708

Start: 2166046

End: 2166696

Strand: Reverse

Name: leuD

Synonym: RSc1989

Alternate gene names: 17546708

Gene position: 2166696-2166046 (Counterclockwise)

Preceding gene: 17546709

Following gene: 17546707

Centisome position: 58.3

GC content: 62.21

Gene sequence:

>651_bases
ATGGAACAATTCACCATCCACACCGGCCTGGTTGCGCCGCTGGACCGCGAGAACGTCGATACCGACGCCATCATTCCGAA
GCAATTCCTCAAGTCGATCAAGCGTACCGGCTTCGGCCCGAACCTGTTCGACGAGTGGCGCTACAAGGACGTGGGCGAGC
CCGGCCAGGACAACAGCAAGCGTCCGCTGAACCCGGACTTCGTGCTGAATCAGCCGCGCTATCAGGGCGCATCGGTCCTG
CTTGCGCGCAAGAACTTCGGCTGCGGCAGCTCGCGCGAGCATGCTCCCTGGGCGCTGCAGCAGTACGGCTTCCGCGCCAT
CATCGCGCCGAGCTTTGCCGACATCTTCTTCAACAACTGCTTCAAGAACGGCCTGCTGCCGATCGTGCTGACGGAGTCGC
AGGTCGACCACCTGTTCAACGAGACGCAGGCCTTCACCGGCTACCAGCTCACTGTGGATCTGGACAAGCAGGTTGTCGTC
ACGCCGGGCGGCACGGCCTATCCGTTCGATGTCACGGCCTTCCGCAAGTACTGCTTGCTCAACGGTTTCGACGACATCGG
CCTGACCCTGCGCTACGCCGATCAGATCAAGGCCTACGAAGCACAACGCCTGGCCAAGATGCCGTGGCTCGCGCACAAGC
TGGTCGGCTGA

Upstream 100 bases:

>100_bases
CGCACCCACCTGGTGAGCCCGGCGATGGCCGCCGCCGCCGCGCTGGAAGGCCACTTCGTCGACGTGCGCAAGCTCGGCTG
ACCGGCAGGAGAGAACGATC

Downstream 100 bases:

>100_bases
TGCCGGCAAATGCATAACAAGGACAAACGCATGACCAAAATTGCAGTGTTGCCGGGTGACGGCATTGGCACGGAAATCGT
GGCCGAGGCCGTCAAGGTGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MEQFTIHTGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNCFKNGLLPIVLTESQVDHLFNETQAFTGYQLTVDLDKQVVV
TPGGTAYPFDVTAFRKYCLLNGFDDIGLTLRYADQIKAYEAQRLAKMPWLAHKLVG

Sequences:

>Translated_216_residues
MEQFTIHTGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNCFKNGLLPIVLTESQVDHLFNETQAFTGYQLTVDLDKQVVV
TPGGTAYPFDVTAFRKYCLLNGFDDIGLTLRYADQIKAYEAQRLAKMPWLAHKLVG
>Mature_216_residues
MEQFTIHTGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGQDNSKRPLNPDFVLNQPRYQGASVL
LARKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNCFKNGLLPIVLTESQVDHLFNETQAFTGYQLTVDLDKQVVV
TPGGTAYPFDVTAFRKYCLLNGFDDIGLTLRYADQIKAYEAQRLAKMPWLAHKLVG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=206, Percent_Identity=59.2233009708738, Blast_Score=243, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6321429, Length=214, Percent_Identity=49.0654205607477, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_RALSO (Q8XXX4)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520110.1
- ProteinModelPortal:   Q8XXX4
- SMR:   Q8XXX4
- GeneID:   1220829
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1989
- NMPDR:   fig|267608.1.peg.1989
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   RSOL267608:RSC1989-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24410; Mature: 24410

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQFTIHTGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGQDNSK
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
RPLNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCEEEECCHHHHHHHHHH
FKNGLLPIVLTESQVDHLFNETQAFTGYQLTVDLDKQVVVTPGGTAYPFDVTAFRKYCLL
HHCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHH
NGFDDIGLTLRYADQIKAYEAQRLAKMPWLAHKLVG
CCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure
MEQFTIHTGLVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNLFDEWRYKDVGEPGQDNSK
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
RPLNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC
CCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCEEEECCHHHHHHHHHH
FKNGLLPIVLTESQVDHLFNETQAFTGYQLTVDLDKQVVVTPGGTAYPFDVTAFRKYCLL
HHCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHH
NGFDDIGLTLRYADQIKAYEAQRLAKMPWLAHKLVG
CCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852