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The map label for this gene is leuB1 [H]

Identifier: 17546707

GI number: 17546707

Start: 2164945

End: 2166036

Strand: Reverse

Name: leuB1 [H]

Synonym: RSc1988

Alternate gene names: 17546707

Gene position: 2166036-2164945 (Counterclockwise)

Preceding gene: 17546708

Following gene: 17546706

Centisome position: 58.28

GC content: 65.48

Gene sequence:

>1092_bases
ATGCATAACAAGGACAAACGCATGACCAAAATTGCAGTGTTGCCGGGTGACGGCATTGGCACGGAAATCGTGGCCGAGGC
CGTCAAGGTGCTCAAGACGCTGGGCGAGACGTTCGAGATGGAAACCGCGCCCGTCGGCGGCGCCGGCTACGAGGCCAAGG
GTCATCCGCTGCCAGAGGACACGCTCAAGCTCGCCAAGGAAGCCGATGCCATCCTGTTCGGCGCCGTCGGCGACTGGAAG
TACGACACGCTGCCGCGCGAACTGCGTCCCGAGCAGGCCATCCTGGGCCTGCGCAAGCACCTGCAACTGTTCGCCAACTT
CCGCCCGGCTATCTGCTATCCGGAGCTGGCCGGTGCATCGAGCATGAAGCCCGAAATCGTGGCGGGGCTCGACATCCTGA
TCGTGCGCGAACTGACCGGCGATATCTACTTCGGCCAGCCGCGCGGCGTGCGTGCCGCACCGGACGGCCTGTTCGCCGGC
GCGCGCGAGGGCTTCGACACCATGCGCTACAGCGAGCCGGAAATCCGTCGCATCGCGCACGTCGCCTTCCAGGCGGCGGC
CAAGCGCGGCAAGAAGCTGTGCAGCGTCGACAAGGCCAATGTGCTGGAGACCTTCCAGTTCTGGAAGGACATTGTCACCG
ACGTGCACAAGGAGTACCCCGAGGTCGAGCTCTCGCACATGTACGTGGACAACGCCGCGATGCAGCTGGTCAAGGCGCCC
AAGAACTTCGACGTGGTTGTCACCGGCAACATGTTCGGCGACATCCTGTCGGACGAGGCCGCGATGCTGACGGGCTCGAT
CGGCATGCTGCCGTCGGCCTCGCTCGATGCCAACAACAAGGGCCTGTACGAGCCGTCGCACGGTTCGGCCCCGGACATCG
CGGGCAAGGGCATCGCCAACCCGCTGGCGACCATCCTGTCGGCGGCGATGATGCTGCGCTACACGCTGGGCAAGGCCGAG
CAGGCGGACCGCATCGAGAACGCGGTCAAGAAGGTGCTGGCCCAGGGCTATCGCACCGGCGACATCCTGACGCCGGGCTG
CAAGCAGGTCGGTACGGTGGAGATGGGCGACGCCGTGGTGGCCGCGCTGTAA

Upstream 100 bases:

>100_bases
CCTGACCCTGCGCTACGCCGATCAGATCAAGGCCTACGAAGCACAACGCCTGGCCAAGATGCCGTGGCTCGCGCACAAGC
TGGTCGGCTGATGCCGGCAA

Downstream 100 bases:

>100_bases
GCCGGACCCCGCGTTGCGGTGCCGTCACATGCGGCACCGCGACCGTGGTTTTTTGCGCGGCGCCATGCTGCACCGCATTG
TGCTGCAGCCAAATCGTGTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MHNKDKRMTKIAVLPGDGIGTEIVAEAVKVLKTLGETFEMETAPVGGAGYEAKGHPLPEDTLKLAKEADAILFGAVGDWK
YDTLPRELRPEQAILGLRKHLQLFANFRPAICYPELAGASSMKPEIVAGLDILIVRELTGDIYFGQPRGVRAAPDGLFAG
AREGFDTMRYSEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAP
KNFDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIANPLATILSAAMMLRYTLGKAE
QADRIENAVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAAL

Sequences:

>Translated_363_residues
MHNKDKRMTKIAVLPGDGIGTEIVAEAVKVLKTLGETFEMETAPVGGAGYEAKGHPLPEDTLKLAKEADAILFGAVGDWK
YDTLPRELRPEQAILGLRKHLQLFANFRPAICYPELAGASSMKPEIVAGLDILIVRELTGDIYFGQPRGVRAAPDGLFAG
AREGFDTMRYSEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAP
KNFDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIANPLATILSAAMMLRYTLGKAE
QADRIENAVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAAL
>Mature_363_residues
MHNKDKRMTKIAVLPGDGIGTEIVAEAVKVLKTLGETFEMETAPVGGAGYEAKGHPLPEDTLKLAKEADAILFGAVGDWK
YDTLPRELRPEQAILGLRKHLQLFANFRPAICYPELAGASSMKPEIVAGLDILIVRELTGDIYFGQPRGVRAAPDGLFAG
AREGFDTMRYSEPEIRRIAHVAFQAAAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAP
KNFDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIANPLATILSAAMMLRYTLGKAE
QADRIENAVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVVAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=31.6109422492401, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI28178821, Length=309, Percent_Identity=29.126213592233, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178816, Length=309, Percent_Identity=28.8025889967638, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4758582, Length=306, Percent_Identity=28.7581699346405, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI28178838, Length=308, Percent_Identity=28.8961038961039, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=36.1702127659575, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=52.3943661971831, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1788101, Length=355, Percent_Identity=34.6478873239437, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=26.3819095477387, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=343, Percent_Identity=30.9037900874636, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=30.8605341246291, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=30.1492537313433, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=29.608938547486, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=45.4794520547945, Blast_Score=278, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=358, Percent_Identity=36.5921787709497, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324291, Length=334, Percent_Identity=31.437125748503, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=342, Percent_Identity=29.5321637426901, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24643270, Length=340, Percent_Identity=32.3529411764706, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24643268, Length=340, Percent_Identity=32.3529411764706, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=30.1169590643275, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161078637, Length=344, Percent_Identity=26.1627906976744, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161078635, Length=344, Percent_Identity=26.1627906976744, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161078633, Length=344, Percent_Identity=26.1627906976744, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24650122, Length=344, Percent_Identity=26.1627906976744, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161078639, Length=342, Percent_Identity=26.3157894736842, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=28.2798833819242, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=28.2798833819242, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI20130355, Length=172, Percent_Identity=30.8139534883721, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39129; Mature: 39129

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNKDKRMTKIAVLPGDGIGTEIVAEAVKVLKTLGETFEMETAPVGGAGYEAKGHPLPED
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
TLKLAKEADAILFGAVGDWKYDTLPRELRPEQAILGLRKHLQLFANFRPAICYPELAGAS
HHHHHHHCCEEEEECCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCC
SMKPEIVAGLDILIVRELTGDIYFGQPRGVRAAPDGLFAGAREGFDTMRYSEPEIRRIAH
CCCCHHHHCCHHEEEEECCCCEEECCCCCCCCCCCCHHHHHHHCHHHHCCCCHHHHHHHH
VAFQAAAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAP
HHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHCC
KNFDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIAN
CCCEEEEECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
PLATILSAAMMLRYTLGKAEQADRIENAVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEECCCCHH
AAL
HCC
>Mature Secondary Structure
MHNKDKRMTKIAVLPGDGIGTEIVAEAVKVLKTLGETFEMETAPVGGAGYEAKGHPLPED
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
TLKLAKEADAILFGAVGDWKYDTLPRELRPEQAILGLRKHLQLFANFRPAICYPELAGAS
HHHHHHHCCEEEEECCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCC
SMKPEIVAGLDILIVRELTGDIYFGQPRGVRAAPDGLFAGAREGFDTMRYSEPEIRRIAH
CCCCHHHHCCHHEEEEECCCCEEECCCCCCCCCCCCHHHHHHHCHHHHCCCCHHHHHHHH
VAFQAAAKRGKKLCSVDKANVLETFQFWKDIVTDVHKEYPEVELSHMYVDNAAMQLVKAP
HHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEHHEEHHHHHHHHHHCC
KNFDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYEPSHGSAPDIAGKGIAN
CCCEEEEECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
PLATILSAAMMLRYTLGKAEQADRIENAVKKVLAQGYRTGDILTPGCKQVGTVEMGDAVV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCEECCCCHH
AAL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]