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The map label for this gene is leuC

Identifier: 17546709

GI number: 17546709

Start: 2166716

End: 2168125

Strand: Reverse

Name: leuC

Synonym: RSc1990

Alternate gene names: 17546709

Gene position: 2168125-2166716 (Counterclockwise)

Preceding gene: 17546710

Following gene: 17546708

Centisome position: 58.34

GC content: 67.94

Gene sequence:

>1410_bases
ATGGCCAAGACGCTCTACGACAAACTCTGGGACGACCACGTCGTCCACACCGAAGAGGACGGCACGACCGTGCTCTACAT
CGACCGCCAGCTGCTGCACGAGGTGACCAGCCCGCAGGCGTTCGAGGGCCTGAAGCTGGCCAACCGCCCGGTCTGGCGCA
TCAGCGCGAACCTGGCCGTCTCGGACCACAACGTGCCGACCACCGACCGCTCGCACGGCATCGCCGACCCGGTCTCCAAG
CTGCAGGTCGACACGCTCGATGCCAACTGCGACAGCTTCGGCATCACGCAATTCAAGATGACCGACAAGCGCCAGGGCAT
CGTGCACGTGATCGGGCCGGAGCAGGGCGCGACGTTGCCGGGCATGACGGTGGTCTGCGGCGACTCGCACACCAGCACGC
ACGGCGCGTTCGGCGCGCTGGCGCACGGCATCGGCACCTCGGAAGTCGAGCACGTGCTGGCCACGCAGACGCTGCTCGCC
AAGAAGAGCAAGAACATGCTGGTGAAGGTCGAAGGCACGCTGCCGCGCGGCTGCACCGCCAAGGACATCGTGCTCGCCAT
CATCGGCAAGATCGGCACGGCGGGCGGCACCGGCTACGCGATGGAGTTCGGTGGCTCGGCCATCCGCGCGCTGTCGATGG
AAGGCCGCATGACGGTGTGCAACATGGCGATCGAGGCGGGCGCGCGCGCCGGCATGGTCGGCGTGGACGACATCACGCTC
GAGTACATCAAGGGCCGTCCGTTCGCGCCGCAGGGCGTGGAATGGGAGCAGGCCGTGGCCTACTGGCGCTCGCTGCACTC
CGATGAGGGTGCCCGCTTCGACCACGTGGTCGAGCTGCGCGCCGAAGAGATCCGCCCGCAGGTGAGCTGGGGCACCTCGC
CGGAAATGGTGGTCAGCATCGAAGACCGCGTGCCGGATCCGGACAAGGAAAAGGACCCGGTCAAGCGCAATGCCATGGAG
CGCGCGCTCGAGTACATGGCCCTGCAGCCCAACGTCGCCATCGGCGACATCCGCATCGACAAGGTCTTCATCGGCTCGTG
CACCAACAGCCGCATCGAGGACATGCGCGCCGCCGCCTGGGTGGTGCAGAAGCTCGGCAAGCGCATTGCCTCCAACGTGA
AGCTGGCCATGGTGGTGCCGGGTTCGGGCCTGGTCAAGGAGCAGGCCGAGCGCGAAGGGCTGGACAAAATTTTCAAGGCC
GCCGGCTTCGAATGGCGCGAGCCGGGCTGCTCGATGTGCCTGGCGATGAACGCCGACCGGCTGGAGCCGGGCGAGCGCTG
CGCGTCCACGTCCAACCGCAATTTCGAAGGCCGGCAGGGCGCGGGCGGCCGCACCCACCTGGTGAGCCCGGCGATGGCCG
CCGCCGCCGCGCTGGAAGGCCACTTCGTCGACGTGCGCAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
GACGTCCAGCCTCGCGGCCGCCGGGTGGCATAATCGGTCACTCCCCACGAAACAGCCCGCGCGGGCGCCCGCACACGGCG
CACTGTGCACGGATCGCATC

Downstream 100 bases:

>100_bases
CCGGCAGGAGAGAACGATCATGGAACAATTCACCATCCACACCGGCCTGGTTGCGCCGCTGGACCGCGAGAACGTCGATA
CCGACGCCATCATTCCGAAG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLANRPVWRISANLAVSDHNVPTTDRSHGIADPVSK
LQVDTLDANCDSFGITQFKMTDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLA
KKSKNMLVKVEGTLPRGCTAKDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGMVGVDDITL
EYIKGRPFAPQGVEWEQAVAYWRSLHSDEGARFDHVVELRAEEIRPQVSWGTSPEMVVSIEDRVPDPDKEKDPVKRNAME
RALEYMALQPNVAIGDIRIDKVFIGSCTNSRIEDMRAAAWVVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKA
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLG

Sequences:

>Translated_469_residues
MAKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLANRPVWRISANLAVSDHNVPTTDRSHGIADPVSK
LQVDTLDANCDSFGITQFKMTDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLA
KKSKNMLVKVEGTLPRGCTAKDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGMVGVDDITL
EYIKGRPFAPQGVEWEQAVAYWRSLHSDEGARFDHVVELRAEEIRPQVSWGTSPEMVVSIEDRVPDPDKEKDPVKRNAME
RALEYMALQPNVAIGDIRIDKVFIGSCTNSRIEDMRAAAWVVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKA
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLG
>Mature_468_residues
AKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLANRPVWRISANLAVSDHNVPTTDRSHGIADPVSKL
QVDTLDANCDSFGITQFKMTDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLAK
KSKNMLVKVEGTLPRGCTAKDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGMVGVDDITLE
YIKGRPFAPQGVEWEQAVAYWRSLHSDEGARFDHVVELRAEEIRPQVSWGTSPEMVVSIEDRVPDPDKEKDPVKRNAMER
ALEYMALQPNVAIGDIRIDKVFIGSCTNSRIEDMRAAAWVVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKAA
GFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=28.099173553719, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI41352693, Length=375, Percent_Identity=26.4, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI8659555, Length=395, Percent_Identity=26.5822784810127, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=63.034188034188, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=28.0653950953678, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=30.4109589041096, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=30.4109589041096, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=303, Percent_Identity=29.7029702970297, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=27.4074074074074, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=63.771186440678, Blast_Score=617, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6320440, Length=355, Percent_Identity=27.3239436619718, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=29.0858725761773, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=477, Percent_Identity=26.2054507337526, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365315, Length=394, Percent_Identity=26.3959390862944, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=394, Percent_Identity=26.3959390862944, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076999, Length=394, Percent_Identity=26.3959390862944, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=400, Percent_Identity=26.5, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645686, Length=379, Percent_Identity=27.9683377308707, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17137564, Length=372, Percent_Identity=26.0752688172043, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_RALSO (Q8XXX3)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520111.1
- ProteinModelPortal:   Q8XXX3
- SMR:   Q8XXX3
- GeneID:   1220830
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1990
- NMPDR:   fig|267608.1.peg.1990
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   RSOL267608:RSC1990-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50797; Mature: 50665

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLANRPVWRISANLAV
CCCHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCHHCCCHHCCCCEEEEEEEEEE
SDHNVPTTDRSHGIADPVSKLQVDTLDANCDSFGITQFKMTDKRQGIVHVIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLAKKSKNMLVKVEGTLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
KDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGMVGVDDITL
HHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEEEEHHHHHCCCCCCCCCCCHHEE
EYIKGRPFAPQGVEWEQAVAYWRSLHSDEGARFDHVVELRAEEIRPQVSWGTSPEMVVSI
HEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEE
EDRVPDPDKEKDPVKRNAMERALEYMALQPNVAIGDIRIDKVFIGSCTNSRIEDMRAAAW
ECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHH
VVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKAAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLG
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
AKTLYDKLWDDHVVHTEEDGTTVLYIDRQLLHEVTSPQAFEGLKLANRPVWRISANLAV
CCHHHHHHCCCCEEEECCCCCEEEEECHHHHHHHCCCCHHCCCHHCCCCEEEEEEEEEE
SDHNVPTTDRSHGIADPVSKLQVDTLDANCDSFGITQFKMTDKRQGIVHVIGPEQGATLP
ECCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLAKKSKNMLVKVEGTLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
KDIVLAIIGKIGTAGGTGYAMEFGGSAIRALSMEGRMTVCNMAIEAGARAGMVGVDDITL
HHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCEEEEHHHHHCCCCCCCCCCCHHEE
EYIKGRPFAPQGVEWEQAVAYWRSLHSDEGARFDHVVELRAEEIRPQVSWGTSPEMVVSI
HEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEE
EDRVPDPDKEKDPVKRNAMERALEYMALQPNVAIGDIRIDKVFIGSCTNSRIEDMRAAAW
ECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHH
VVQKLGKRIASNVKLAMVVPGSGLVKEQAEREGLDKIFKAAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAALEGHFVDVRKLG
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852