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The map label for this gene is hpaIM [H]

Identifier: 17546701

GI number: 17546701

Start: 2156516

End: 2157328

Strand: Reverse

Name: hpaIM [H]

Synonym: RSc1982

Alternate gene names: 17546701

Gene position: 2157328-2156516 (Counterclockwise)

Preceding gene: 17546702

Following gene: 17546700

Centisome position: 58.05

GC content: 67.9

Gene sequence:

>813_bases
ATGACCAAGCGCGCCATCGACGGCATCTTCAACGAGGACTGCATCGCCGGTGTCGGCCGCCTGGCCGACGGCAGCGTGGA
CCTCGTCATCGCCGATCCGCCGTACGGGCTGGGCAAGGACTATGGCAACGACTCCGACAAGCTGTCGGGCGAGGCCTACC
TGGCCTGGTCGGAACGCTGGATCGAGGCGGTGTTGCCGAAGCTGGCGCGCAACGGCTCGCTCTACCTGTTCTGCACGTGG
CAGTACGCGCCGGAGCTGTTCGTGATGCTCAAGCGGCGCCTGTCGATGATCAACGAGATCATCTGGGACCGCCGCGTGCC
CAGCATGGGCGGCAGCACGCGCAAGTTCTCTTCGGTGCACGACAACATCGGCTTCTTCGCCGCCTCGCGCGACTACTACT
TCGATGTCGACGCCGTGCGCATCCCGTATGACGCCGAGACCAAGAAGGCCCGCAGCCGCAAGCGCTTCGAGGGCAAGAAG
TGGCTGGAGGTCGGCTACAACCCGAAGGACGTGTGGAGCGTGTCGCGCCTGCATCGGCAGGACCCCGAACGCGCCGAGCA
CCCGACGCAGAAGCCGCTGGAACTGGTCGAGCGCATGGTGCTGGCGAGCTGCCCGCCGGGCGGGCTGGTGCTGGACCCGT
TCCTGGGCAGCGGTACCACCGCCGCGGCCTGCGCGCGGCTGGGCCGCCGGTTTGCCGGCTTCGAGATCAACGCCGAGTAT
TGCCGCGTGGCGCGCGAGCGGGTGGCCGCCGTCACGCCGCTTGGACCCCAATCGACTGATTCCCAGGTGCCCGACACCGT
CAGGGTCGCCTGA

Upstream 100 bases:

>100_bases
ACGCTGCCGAAGGACAAGATCCTGCTGGTCAACCTGTCCGGCCGCGGCGACAAGGACATGCATACCGTCGCCGAACTCTC
GGGCATCGATCTCTGACGCA

Downstream 100 bases:

>100_bases
AACCTCTTCAATCCATGTCCCGCATTGCTCAAACTTTTTCGCAATTGTCCGCGCAGGGCCGCAAGGGCCTGATTCCGTTC
ATCACGGCGGGCGACCCGTA

Product: DNA-methyltransferase (DNA-modification methylase) protein

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTKRAIDGIFNEDCIAGVGRLADGSVDLVIADPPYGLGKDYGNDSDKLSGEAYLAWSERWIEAVLPKLARNGSLYLFCTW
QYAPELFVMLKRRLSMINEIIWDRRVPSMGGSTRKFSSVHDNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKK
WLEVGYNPKDVWSVSRLHRQDPERAEHPTQKPLELVERMVLASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINAEY
CRVARERVAAVTPLGPQSTDSQVPDTVRVA

Sequences:

>Translated_270_residues
MTKRAIDGIFNEDCIAGVGRLADGSVDLVIADPPYGLGKDYGNDSDKLSGEAYLAWSERWIEAVLPKLARNGSLYLFCTW
QYAPELFVMLKRRLSMINEIIWDRRVPSMGGSTRKFSSVHDNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKK
WLEVGYNPKDVWSVSRLHRQDPERAEHPTQKPLELVERMVLASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINAEY
CRVARERVAAVTPLGPQSTDSQVPDTVRVA
>Mature_269_residues
TKRAIDGIFNEDCIAGVGRLADGSVDLVIADPPYGLGKDYGNDSDKLSGEAYLAWSERWIEAVLPKLARNGSLYLFCTWQ
YAPELFVMLKRRLSMINEIIWDRRVPSMGGSTRKFSSVHDNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKKW
LEVGYNPKDVWSVSRLHRQDPERAEHPTQKPLELVERMVLASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINAEYC
RVARERVAAVTPLGPQSTDSQVPDTVRVA

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=246, Percent_Identity=30.8943089430894, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30227; Mature: 30095

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRAIDGIFNEDCIAGVGRLADGSVDLVIADPPYGLGKDYGNDSDKLSGEAYLAWSERW
CCCHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHH
IEAVLPKLARNGSLYLFCTWQYAPELFVMLKRRLSMINEIIWDRRVPSMGGSTRKFSSVH
HHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
DNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKKWLEVGYNPKDVWSVSRLHRQ
CCCCEEEECCCEEEECCEEECCCCCHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHC
DPERAEHPTQKPLELVERMVLASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINAEY
CCHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEECHHH
CRVARERVAAVTPLGPQSTDSQVPDTVRVA
HHHHHHHHHHCCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
TKRAIDGIFNEDCIAGVGRLADGSVDLVIADPPYGLGKDYGNDSDKLSGEAYLAWSERW
CCHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHH
IEAVLPKLARNGSLYLFCTWQYAPELFVMLKRRLSMINEIIWDRRVPSMGGSTRKFSSVH
HHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
DNIGFFAASRDYYFDVDAVRIPYDAETKKARSRKRFEGKKWLEVGYNPKDVWSVSRLHRQ
CCCCEEEECCCEEEECCEEECCCCCHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHC
DPERAEHPTQKPLELVERMVLASCPPGGLVLDPFLGSGTTAAACARLGRRFAGFEINAEY
CCHHHCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCEEECHHH
CRVARERVAAVTPLGPQSTDSQVPDTVRVA
HHHHHHHHHHCCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]