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The map label for this gene is trpB

Identifier: 17546702

GI number: 17546702

Start: 2157333

End: 2158544

Strand: Reverse

Name: trpB

Synonym: RSc1983

Alternate gene names: 17546702

Gene position: 2158544-2157333 (Counterclockwise)

Preceding gene: 17546703

Following gene: 17546701

Centisome position: 58.08

GC content: 67.0

Gene sequence:

>1212_bases
ATGCCTCGAGAGATTGCCATGTACAACCTGCCCGACGCCCACGGCCACTTCGGCCCTTACGGCGGCACCTTCGTTGCGGA
AACGCTGTCCCACGCGCTGGACGAGCTGCGCGATGCCTATGCGCGCTATCAGCACGATCCCGAATTCATCAAGGAATACG
AGTACGAGCTGAAGCATTTCGTCGGCCGTCCGTCGCCCATCTACCATGCCCGCCGCCTGACCGAGCATTGCGGCGGCGCG
CAGATCTACCTCAAGCGCGAAGACCTGAACCACACCGGCGCGCACAAGGTGAACAACGTCATCGGCCAGGCGCTGCTGGC
GCGCCGCATGGGCAAGCCGCGCGTGATCGCCGAGACCGGCGCCGGCCAGCACGGCGTGGCCACCGCCACCATCGCCGCGC
GCTACGGCATGGAGTGCGTGGTCTACATGGGCAGCGAGGACGTGCGCCGGCAGGCCGCCAACGTCTATCGCATGAAGCTG
CTGGGCGCGACCGTGGTGCCGGTGGAGTCCGGTTCGCGCACGCTCAAGGACGCGCTGAATGAAGCGATGCGCGACTGGGT
CACCAACGTGGCCGACACCTTCTACATCATCGGCACCGTGGCCGGTCCGCACCCGTATCCGATGATGGTGCGCGACTTCC
AGGCCGTGATCGGCGAGGAATGCAAGGTGCAGATGCCCGAGCTGGCCGGCCGCCAGCCGGACGCCGTGATCGCCTGCGTG
GGCGGCGGCTCCAACGCCATGGGCATTTTCTATCCGTACATCGACCATGCCAGCGTGCAGTTGATCGGCGTGGAAGCGGC
GGGCGAGGGCCTCGAGTCGGGCCGGCATGCCGCGTCGCTCACGGGCGGCTCGCCGGGCGTGCTGCACGGCAACCGCACCT
ATCTGCTGCAGGACGAGGACGGCCAGATCATCGAGACGCATTCGATCTCGGCCGGCCTGGACTACCCCGGTGTCGGCCCC
GAGCATGCCTGGCTGAAGGACGCGGGTCGCGCGCAGTATGTCGGCATCACCGACAAGGAAGCGCTGCAGGCCTTCCACGA
CCTGTGCCGCATGGAGGGCATCATCCCCGCGCTGGAGTCCAGCCATGCGCTGGCCTACGCCTGCAAGCTGGCGCCGACGC
TGCCGAAGGACAAGATCCTGCTGGTCAACCTGTCCGGCCGCGGCGACAAGGACATGCATACCGTCGCCGAACTCTCGGGC
ATCGATCTCTGA

Upstream 100 bases:

>100_bases
TGGGCGCGTCGGTGCAAGCCTGAGCCACATTGGCCGGTCTCTCGCGGTTCGCACCTTCCTGAATATCGCCGCCGGCCGGT
CCCTGGTCTGAGCCCGGCGG

Downstream 100 bases:

>100_bases
CGCAATGACCAAGCGCGCCATCGACGGCATCTTCAACGAGGACTGCATCGCCGGTGTCGGCCGCCTGGCCGACGGCAGCG
TGGACCTCGTCATCGCCGAT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPREIAMYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHFVGRPSPIYHARRLTEHCGGA
QIYLKREDLNHTGAHKVNNVIGQALLARRMGKPRVIAETGAGQHGVATATIAARYGMECVVYMGSEDVRRQAANVYRMKL
LGATVVPVESGSRTLKDALNEAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPELAGRQPDAVIACV
GGGSNAMGIFYPYIDHASVQLIGVEAAGEGLESGRHAASLTGGSPGVLHGNRTYLLQDEDGQIIETHSISAGLDYPGVGP
EHAWLKDAGRAQYVGITDKEALQAFHDLCRMEGIIPALESSHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSG
IDL

Sequences:

>Translated_403_residues
MPREIAMYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHFVGRPSPIYHARRLTEHCGGA
QIYLKREDLNHTGAHKVNNVIGQALLARRMGKPRVIAETGAGQHGVATATIAARYGMECVVYMGSEDVRRQAANVYRMKL
LGATVVPVESGSRTLKDALNEAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPELAGRQPDAVIACV
GGGSNAMGIFYPYIDHASVQLIGVEAAGEGLESGRHAASLTGGSPGVLHGNRTYLLQDEDGQIIETHSISAGLDYPGVGP
EHAWLKDAGRAQYVGITDKEALQAFHDLCRMEGIIPALESSHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSG
IDL
>Mature_402_residues
PREIAMYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHFVGRPSPIYHARRLTEHCGGAQ
IYLKREDLNHTGAHKVNNVIGQALLARRMGKPRVIAETGAGQHGVATATIAARYGMECVVYMGSEDVRRQAANVYRMKLL
GATVVPVESGSRTLKDALNEAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPELAGRQPDAVIACVG
GGSNAMGIFYPYIDHASVQLIGVEAAGEGLESGRHAASLTGGSPGVLHGNRTYLLQDEDGQIIETHSISAGLDYPGVGPE
HAWLKDAGRAQYVGITDKEALQAFHDLCRMEGIIPALESSHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSGI
DL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=384, Percent_Identity=55.9895833333333, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321412, Length=383, Percent_Identity=57.1801566579634, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_RALSO (Q8XXY0)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520104.1
- ProteinModelPortal:   Q8XXY0
- SMR:   Q8XXY0
- GeneID:   1220823
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1983
- NMPDR:   fig|267608.1.peg.1983
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   RSOL267608:RSC1983-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43801; Mature: 43670

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPREIAMYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHF
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VGRPSPIYHARRLTEHCGGAQIYLKREDLNHTGAHKVNNVIGQALLARRMGKPRVIAETG
CCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
AGQHGVATATIAARYGMECVVYMGSEDVRRQAANVYRMKLLGATVVPVESGSRTLKDALN
CCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
EAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPELAGRQPDAVIACV
HHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEECHHHCCCCCCEEEEEE
GGGSNAMGIFYPYIDHASVQLIGVEAAGEGLESGRHAASLTGGSPGVLHGNRTYLLQDED
CCCCCCEEEEEEECCCCEEEEEEEECCCCCHHCCCCEEEECCCCCCEEECCEEEEEECCC
GQIIETHSISAGLDYPGVGPEHAWLKDAGRAQYVGITDKEALQAFHDLCRMEGIIPALES
CCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHCC
SHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSGIDL
CCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
PREIAMYNLPDAHGHFGPYGGTFVAETLSHALDELRDAYARYQHDPEFIKEYEYELKHF
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VGRPSPIYHARRLTEHCGGAQIYLKREDLNHTGAHKVNNVIGQALLARRMGKPRVIAETG
CCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
AGQHGVATATIAARYGMECVVYMGSEDVRRQAANVYRMKLLGATVVPVESGSRTLKDALN
CCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
EAMRDWVTNVADTFYIIGTVAGPHPYPMMVRDFQAVIGEECKVQMPELAGRQPDAVIACV
HHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEECHHHCCCCCCEEEEEE
GGGSNAMGIFYPYIDHASVQLIGVEAAGEGLESGRHAASLTGGSPGVLHGNRTYLLQDED
CCCCCCEEEEEEECCCCEEEEEEEECCCCCHHCCCCEEEECCCCCCEEECCEEEEEECCC
GQIIETHSISAGLDYPGVGPEHAWLKDAGRAQYVGITDKEALQAFHDLCRMEGIIPALES
CCEEEEECCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHCC
SHALAYACKLAPTLPKDKILLVNLSGRGDKDMHTVAELSGIDL
CCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852