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The map label for this gene is nitA [H]

Identifier: 17546542

GI number: 17546542

Start: 1991501

End: 1992532

Strand: Reverse

Name: nitA [H]

Synonym: RSc1823

Alternate gene names: 17546542

Gene position: 1992532-1991501 (Counterclockwise)

Preceding gene: 17546543

Following gene: 17546541

Centisome position: 53.61

GC content: 68.22

Gene sequence:

>1032_bases
ATGCTTGAACTCCCGCAATTCAAGGCGGCCGCCGTGCAGGCAGCGCCGGTCTTTCTCGACACCGATGCCACCGTCGACAA
AGTGTGCAGGCTGATCCAGGAAGCCGCCGAGAACGGCGCGCGCCTGGTGGCCTTTCCCGAAGTGTTCGTGGCCGGCTACC
CCTACTGGAGCTGGATCATGAATCCGGTCGAGGGCAGCCCGTGGTTCGAAAAGCTCGCGCGCTCGGCCATCGAGCTGCCC
GGCCCGGAGATCGCCAAGATCGCCCAGGCCGCCGCGCGCCACCGGATCAACGTGGTGATCGGGGTCAACGAGCGCAGCCG
CTACGGCATCGGCACGCTCTACAACACGATCGTCACCATCGCGGACGACGGCCGCATCCTCGGCCGCCACCGCAAGCTGG
TGCCGACCTGGGCCGAAAAGCTGACCTGGGCCCCCGGCGACGCCTCCGCCTTGCGCGTGCACGACACCAGCATCGGCCCG
CTGGGCGCGCTGGCCTGCGGCGAGAACACCAACACGCTGGCCCGCTTCGCGCTGCTCGCGCAGGGCGAGCTGGTGCACGT
GGCCAGCTACATCTCGCTGCCGGTGGCGCCGCCCGACTACGACATGGCCGAAGCCATCAAGGTCCGTGCGGCGGCCCACT
GCTTCGAGGGCAAGGTCTTCACTGTGGTGTCCTGCTCGACCGTCTCCGAGGAGATCATCGAGGCCATGGCCGCCACCCAT
CCGCAATCGCGCGCGCTCCTGGCGCGGCGCAACAGTGCCTTCTCGGGCATCCTCGGGCCGGACGGCCGCGTCATCGGCCA
GCCGCTGATCGACCAGGAGGGCATCGTCTACGCCGACATCGATCTCTCGCGCTGCATCCAGCCGCGCCAGATGCACGACA
TCACCGGCCACTACAACCGTTTCGACATCTTCGACCTGCGCGTCAACCGCAAGCCGCTGCGGGCCGCGCAGTTCGACGAC
GCCATCGACCCGGACAGCGGCGCCATCGCCGCTGAATCCGCCCCCGACTCGAACCTGGAGAATCTCTCGTGA

Upstream 100 bases:

>100_bases
CGCCCGGGCGCCCAAACCTGTGCCCGGGCTGTCGAACCCCCGCTCTGCCCGCCGCGAGGGCGCATCAGGCGCCTCCGCGC
TTTTCATTCCGGACACCATC

Downstream 100 bases:

>100_bases
GTACCGCTCGCGCCTACCGCATCGGCCAGATCGTGCCGTCCTCGAACACCACCATGGAGACCGAAATTCCGGCCATCCTG
CGTGCCCGCGAATTGACGCA

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLELPQFKAAAVQAAPVFLDTDATVDKVCRLIQEAAENGARLVAFPEVFVAGYPYWSWIMNPVEGSPWFEKLARSAIELP
GPEIAKIAQAAARHRINVVIGVNERSRYGIGTLYNTIVTIADDGRILGRHRKLVPTWAEKLTWAPGDASALRVHDTSIGP
LGALACGENTNTLARFALLAQGELVHVASYISLPVAPPDYDMAEAIKVRAAAHCFEGKVFTVVSCSTVSEEIIEAMAATH
PQSRALLARRNSAFSGILGPDGRVIGQPLIDQEGIVYADIDLSRCIQPRQMHDITGHYNRFDIFDLRVNRKPLRAAQFDD
AIDPDSGAIAAESAPDSNLENLS

Sequences:

>Translated_343_residues
MLELPQFKAAAVQAAPVFLDTDATVDKVCRLIQEAAENGARLVAFPEVFVAGYPYWSWIMNPVEGSPWFEKLARSAIELP
GPEIAKIAQAAARHRINVVIGVNERSRYGIGTLYNTIVTIADDGRILGRHRKLVPTWAEKLTWAPGDASALRVHDTSIGP
LGALACGENTNTLARFALLAQGELVHVASYISLPVAPPDYDMAEAIKVRAAAHCFEGKVFTVVSCSTVSEEIIEAMAATH
PQSRALLARRNSAFSGILGPDGRVIGQPLIDQEGIVYADIDLSRCIQPRQMHDITGHYNRFDIFDLRVNRKPLRAAQFDD
AIDPDSGAIAAESAPDSNLENLS
>Mature_343_residues
MLELPQFKAAAVQAAPVFLDTDATVDKVCRLIQEAAENGARLVAFPEVFVAGYPYWSWIMNPVEGSPWFEKLARSAIELP
GPEIAKIAQAAARHRINVVIGVNERSRYGIGTLYNTIVTIADDGRILGRHRKLVPTWAEKLTWAPGDASALRVHDTSIGP
LGALACGENTNTLARFALLAQGELVHVASYISLPVAPPDYDMAEAIKVRAAAHCFEGKVFTVVSCSTVSEEIIEAMAATH
PQSRALLARRNSAFSGILGPDGRVIGQPLIDQEGIVYADIDLSRCIQPRQMHDITGHYNRFDIFDLRVNRKPLRAAQFDD
AIDPDSGAIAAESAPDSNLENLS

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=323, Percent_Identity=25.077399380805, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17557111, Length=310, Percent_Identity=33.2258064516129, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322027, Length=184, Percent_Identity=33.695652173913, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 37250; Mature: 37250

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELPQFKAAAVQAAPVFLDTDATVDKVCRLIQEAAENGARLVAFPEVFVAGYPYWSWIM
CCCCCCCHHHHHEECCEEEECCCCHHHHHHHHHHHHHCCCEEEEECHHHHCCCCHHHHHC
NPVEGSPWFEKLARSAIELPGPEIAKIAQAAARHRINVVIGVNERSRYGIGTLYNTIVTI
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHEEEEE
ADDGRILGRHRKLVPTWAEKLTWAPGDASALRVHDTSIGPLGALACGENTNTLARFALLA
ECCCEEECCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHH
QGELVHVASYISLPVAPPDYDMAEAIKVRAAAHCFEGKVFTVVSCSTVSEEIIEAMAATH
CCCEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCC
PQSRALLARRNSAFSGILGPDGRVIGQPLIDQEGIVYADIDLSRCIQPRQMHDITGHYNR
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCHHHHHHCCCCCE
FDIFDLRVNRKPLRAAQFDDAIDPDSGAIAAESAPDSNLENLS
EEEEEEEECCCCCCHHCCCCCCCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MLELPQFKAAAVQAAPVFLDTDATVDKVCRLIQEAAENGARLVAFPEVFVAGYPYWSWIM
CCCCCCCHHHHHEECCEEEECCCCHHHHHHHHHHHHHCCCEEEEECHHHHCCCCHHHHHC
NPVEGSPWFEKLARSAIELPGPEIAKIAQAAARHRINVVIGVNERSRYGIGTLYNTIVTI
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHEEEEE
ADDGRILGRHRKLVPTWAEKLTWAPGDASALRVHDTSIGPLGALACGENTNTLARFALLA
ECCCEEECCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHH
QGELVHVASYISLPVAPPDYDMAEAIKVRAAAHCFEGKVFTVVSCSTVSEEIIEAMAATH
CCCEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCC
PQSRALLARRNSAFSGILGPDGRVIGQPLIDQEGIVYADIDLSRCIQPRQMHDITGHYNR
CHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCHHHHHHCCCCCE
FDIFDLRVNRKPLRAAQFDDAIDPDSGAIAAESAPDSNLENLS
EEEEEEEECCCCCCHHCCCCCCCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]