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The map label for this gene is ntrC [H]

Identifier: 17545980

GI number: 17545980

Start: 1340121

End: 1341629

Strand: Direct

Name: ntrC [H]

Synonym: RSc1261

Alternate gene names: 17545980

Gene position: 1340121-1341629 (Clockwise)

Preceding gene: 17545979

Following gene: 17545983

Centisome position: 36.06

GC content: 69.18

Gene sequence:

>1509_bases
ATGAAGCCAATCTGGATCGTCGACGACGATCAATCCATCCGCTGGGTCCTCGAGAAAGCGCTGGCGCGGGAGAGCCTGCT
GTCGCGCAGCTTCACCAATGCTCGCGACGCGCTCAACGCGCTCGAAGAAGAAACGCCGCAGGTGCTGATCTCCGACGTCC
GCATGCCGGGCGGCTCGGGGCTGGACCTGCTGCAGGCCATCAAGGGCCGGCATCCGGGCCTGCCGGTCATCATCATGACG
GCGTATTCCGACCTGGACAGCGCCGTGGCGGCGTTCCAGGGCGGCGCCTTCGAATATCTCGCCAAGCCGTTCGACGTGGA
CCGCGCGGTCGAGCTGATCCGCCGCGCGCTGGAAGAGAGCCTGCGCGAGGAAGAGATCGACGACCGGTTGGGCGATGCGC
CCGAGATCCTCGGTCAGGCGCCGGCGATGCAGGATGTGTTCCGCGCCATCGGCCGGCTGTCGCAGTCGAACGTGACGGTG
CTGATCACCGGCGAGTCCGGCACCGGCAAGGAGCTGGTGGCGCGCGCGCTGCACAAGCACAGCCCGCGGGCAAGCGGCCC
GTTCATCGCGCTCAATACCGCGGCGATCCCCAAAGACCTGCTGGAATCCGAGCTGTTCGGCCATGAGCGTGGCGCCTTTA
CCGGCGCGCAGACCATGCGTCGCGGACGCTTCGAGCAGGCCGAGGGCGGCACGCTGTTCCTCGATGAGATCGGCGACATG
CCGTTCGACCTGCAGACGCGCCTGCTGCGCGTGCTGTCGGACGGGCATTTCTACCGGGTGGGCGGCCACAACCCGCTCAA
GGCCAACGTGCGCGTCATCGCGGCCACGCACCAGAACCTGGAAACGCGCGTCAAGGACGGCCTGTTCCGCGAAGACCTGT
TCCACCGCCTCAACGTGATCCGCCTGCGGCTGCCGGCGTTGCGCGAGCGTCCGGAAGACATCACGCTGCTGGCGCGGCAC
TTCCTGCAGAAGAGCGCGAAGGAGCTGGGCGTGGAGCCCAAGCGCATGTCGGACGAGGCGTTGTCGCACATCGCCACGCT
GCCGTTCCCGGGCAACGTGCGCCAGCTCGAGAACCTCTGCAACTGGCTGACCGTGATGGCGCCGGCGCAGACCATCGAGA
TCAAGGATCTGCCGCATGACCTCGTGCGCGGCGCCAGCCAGATGCTGCCGGGCACGGCCGAGCTGATGGTGGCGGGGCGT
GCGGTGGCGGGCGAGCCGTCTTTCGGCGCCGATGCCCATGCCGCCGGCGCCGAGGCACCCGTGGCCGCGGGCGCGGCGGC
GGGGGCTTCCGCTATGTCCGGCTGGGAGCGCGCGCTGGCCGGCGAGGCCAAGGCGATGCTCGAGGCCGGCCAGCCCGATG
TCATGGATGCTCTGACCCGCCGCTTCGAGCGGGCCATCCTCGAAGCCGCCCTCGGCGTCACGCGTGGGCGCCGGGTCGAA
GCGGCCACGCGCCTGGGCATCGGCCGCAACACCATCACCCGCAAATTGCAGGAGCTCGGCTTCGACTGA

Upstream 100 bases:

>100_bases
CAGCAGGCCGGGCCTGACCGATTTCCGCATCCTGGTGCCGCTGCATTGACGGCGGCGGCATCGGGCCCAGGCGATCTGAA
GTCAAGCTGACACACAACAC

Downstream 100 bases:

>100_bases
CACCGCAGCGAAGCCGGTCCAACCGGATATCGTCGAGCTTGGCGATTGGGATTGGGCGGATTTCCGCAAGGGAATCCGCC
TCTTTTTTTATCTACAGGGT

Product: nitrogen assimilation regulatory response regulator transcription regulator protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNARDALNALEEETPQVLISDVRMPGGSGLDLLQAIKGRHPGLPVIIMT
AYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEESLREEEIDDRLGDAPEILGQAPAMQDVFRAIGRLSQSNVTV
LITGESGTGKELVARALHKHSPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVIRLRLPALRERPEDITLLARH
FLQKSAKELGVEPKRMSDEALSHIATLPFPGNVRQLENLCNWLTVMAPAQTIEIKDLPHDLVRGASQMLPGTAELMVAGR
AVAGEPSFGADAHAAGAEAPVAAGAAAGASAMSGWERALAGEAKAMLEAGQPDVMDALTRRFERAILEAALGVTRGRRVE
AATRLGIGRNTITRKLQELGFD

Sequences:

>Translated_502_residues
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNARDALNALEEETPQVLISDVRMPGGSGLDLLQAIKGRHPGLPVIIMT
AYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEESLREEEIDDRLGDAPEILGQAPAMQDVFRAIGRLSQSNVTV
LITGESGTGKELVARALHKHSPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVIRLRLPALRERPEDITLLARH
FLQKSAKELGVEPKRMSDEALSHIATLPFPGNVRQLENLCNWLTVMAPAQTIEIKDLPHDLVRGASQMLPGTAELMVAGR
AVAGEPSFGADAHAAGAEAPVAAGAAAGASAMSGWERALAGEAKAMLEAGQPDVMDALTRRFERAILEAALGVTRGRRVE
AATRLGIGRNTITRKLQELGFD
>Mature_502_residues
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNARDALNALEEETPQVLISDVRMPGGSGLDLLQAIKGRHPGLPVIIMT
AYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEESLREEEIDDRLGDAPEILGQAPAMQDVFRAIGRLSQSNVTV
LITGESGTGKELVARALHKHSPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVIRLRLPALRERPEDITLLARH
FLQKSAKELGVEPKRMSDEALSHIATLPFPGNVRQLENLCNWLTVMAPAQTIEIKDLPHDLVRGASQMLPGTAELMVAGR
AVAGEPSFGADAHAAGAEAPVAAGAAAGASAMSGWERALAGEAKAMLEAGQPDVMDALTRRFERAILEAALGVTRGRRVE
AATRLGIGRNTITRKLQELGFD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=502, Percent_Identity=57.1713147410359, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1788550, Length=505, Percent_Identity=40.5940594059406, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1790437, Length=383, Percent_Identity=43.0809399477807, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1788905, Length=369, Percent_Identity=44.1734417344173, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI87082117, Length=391, Percent_Identity=39.8976982097187, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789087, Length=244, Percent_Identity=50, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1789233, Length=260, Percent_Identity=45, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=47.6595744680851, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=253, Percent_Identity=48.6166007905138, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=367, Percent_Identity=35.6948228882834, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=46.4601769911504, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789828, Length=211, Percent_Identity=36.9668246445498, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI87081858, Length=326, Percent_Identity=28.5276073619632, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790860, Length=101, Percent_Identity=33.6633663366337, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=28.9473684210526, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54702; Mature: 54702

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNARDALNALEEETPQVLISDVRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCC
LDLLQAIKGRHPGLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEES
HHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LREEEIDDRLGDAPEILGQAPAMQDVFRAIGRLSQSNVTVLITGESGTGKELVARALHKH
HHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHC
SPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHCCC
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVI
CHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHCHHHHHHHHHHHHH
RLRLPALRERPEDITLLARHFLQKSAKELGVEPKRMSDEALSHIATLPFPGNVRQLENLC
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHCCCCCCCCHHHHHHHH
NWLTVMAPAQTIEIKDLPHDLVRGASQMLPGTAELMVAGRAVAGEPSFGADAHAAGAEAP
HHHHHHCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
VAAGAAAGASAMSGWERALAGEAKAMLEAGQPDVMDALTRRFERAILEAALGVTRGRRVE
CHHCHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AATRLGIGRNTITRKLQELGFD
HHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARESLLSRSFTNARDALNALEEETPQVLISDVRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCC
LDLLQAIKGRHPGLPVIIMTAYSDLDSAVAAFQGGAFEYLAKPFDVDRAVELIRRALEES
HHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LREEEIDDRLGDAPEILGQAPAMQDVFRAIGRLSQSNVTVLITGESGTGKELVARALHKH
HHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHC
SPRASGPFIALNTAAIPKDLLESELFGHERGAFTGAQTMRRGRFEQAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHCCC
PFDLQTRLLRVLSDGHFYRVGGHNPLKANVRVIAATHQNLETRVKDGLFREDLFHRLNVI
CHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHCHHHHHHHHHHHHH
RLRLPALRERPEDITLLARHFLQKSAKELGVEPKRMSDEALSHIATLPFPGNVRQLENLC
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHCCCCCCCCHHHHHHHH
NWLTVMAPAQTIEIKDLPHDLVRGASQMLPGTAELMVAGRAVAGEPSFGADAHAAGAEAP
HHHHHHCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
VAAGAAAGASAMSGWERALAGEAKAMLEAGQPDVMDALTRRFERAILEAALGVTRGRRVE
CHHCHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AATRLGIGRNTITRKLQELGFD
HHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]