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The map label for this gene is ntrB [H]

Identifier: 17545979

GI number: 17545979

Start: 1338984

End: 1340069

Strand: Direct

Name: ntrB [H]

Synonym: RSc1260

Alternate gene names: 17545979

Gene position: 1338984-1340069 (Clockwise)

Preceding gene: 17545978

Following gene: 17545980

Centisome position: 36.03

GC content: 67.68

Gene sequence:

>1086_bases
ATGCCGCCTCTGCCGTTCTCGCACAGTGCGCCGCCGTCGTATCCCGGGCTCGATGCCGTGCCCAATCCCGTCCTCTTCGT
CCGCCATCCGTCGCTGACGATCTTCTACGCCAATCCCGCCGCCGAGGCCACGCTGGCCGTGTCGCGCCGCCAACTGGTGG
GCACGTCGCTGCACGAGCTGTTCGCCGATCCGGTCGAGCTGATGGAGATGATCGGCACGGTGGCGAGCCGCCTGTTCGAC
GCCAAGCGCCGCGATCTGACGCTCGAGCGGCCGGGCCACGAGGCGCTGCACGTGCACGCGGTGGTCGGCACCATCGATTT
CGAGCCGGGCTGCGCCCTGCTGGAGCTGCTGCCCAACGAGCAGAAGATGCGCAGCGAGCGCGAAGAGCGCCTGCTGGACC
TCACCTCCGCCAACAAGGAACTGATCCGCAACCTGGCGCACGAGATCAAGAACCCGCTGGGCGGCATCCGCGGCGCGGCG
CAGTTGCTGGAGTTCGAGCTGCCCGAGCGTGCGCTGCGCGAGTACACCCAGGTCATCATCAAGGAGTCCGACCGGCTGCA
GACGCTGGTGGACCGCCTGCTGGAGCCCCACCGGCATCCGCACATCGTCGGCGACGTGAACATCCACGAGGTGCTCGAGC
GCGTGCGCTCGGTGGTGCTGGCGGAGTTTCCGCAGGGCCTGCGCATCGTGCGCGACTACGACGCCAGCCTGCCCGAGTTC
CGCGGCGACAAGGAGCAACTGATCCAGGCCGTGCTCAACATCGTCCACAACGCGGCGCATGCCCTGGGGCCGCGCATCGC
GCAGGGCAATGCCGAAATCATTCTGCGCACGCGGGTGGCGCGCCAGGTCACGATCGCCAAGCATCTCTACAAGCTGGCAT
TGGACTTGCATGTGGTGGACAACGGTCCTGGTATCCCGGACGACATCCGCGAGCGCATCTTCTTCCCGCTGGTGTCCGGA
CGGGAGGGGGGGAGCGGGCTGGGCCTGACGCTGGCGCAGACCTTCGTGCAGCAGCACGACGGCCTGATCGAATGCGACAG
CAGGCCGGGCCTGACCGATTTCCGCATCCTGGTGCCGCTGCATTGA

Upstream 100 bases:

>100_bases
CCGCAAAGCGGCCCACCGCGATGACCAGGAACAGACGACGCCATCCGCGCCGCCCACCGCCGCTCCGGAAGCTCCGGCCG
GCGGCCGCGCCGCGTCGGCG

Downstream 100 bases:

>100_bases
CGGCGGCGGCATCGGGCCCAGGCGATCTGAAGTCAAGCTGACACACAACACATGAAGCCAATCTGGATCGTCGACGACGA
TCAATCCATCCGCTGGGTCC

Product: nitrogen regulation(sensor protein kinase transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MPPLPFSHSAPPSYPGLDAVPNPVLFVRHPSLTIFYANPAAEATLAVSRRQLVGTSLHELFADPVELMEMIGTVASRLFD
AKRRDLTLERPGHEALHVHAVVGTIDFEPGCALLELLPNEQKMRSEREERLLDLTSANKELIRNLAHEIKNPLGGIRGAA
QLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVLERVRSVVLAEFPQGLRIVRDYDASLPEF
RGDKEQLIQAVLNIVHNAAHALGPRIAQGNAEIILRTRVARQVTIAKHLYKLALDLHVVDNGPGIPDDIRERIFFPLVSG
REGGSGLGLTLAQTFVQQHDGLIECDSRPGLTDFRILVPLH

Sequences:

>Translated_361_residues
MPPLPFSHSAPPSYPGLDAVPNPVLFVRHPSLTIFYANPAAEATLAVSRRQLVGTSLHELFADPVELMEMIGTVASRLFD
AKRRDLTLERPGHEALHVHAVVGTIDFEPGCALLELLPNEQKMRSEREERLLDLTSANKELIRNLAHEIKNPLGGIRGAA
QLLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVLERVRSVVLAEFPQGLRIVRDYDASLPEF
RGDKEQLIQAVLNIVHNAAHALGPRIAQGNAEIILRTRVARQVTIAKHLYKLALDLHVVDNGPGIPDDIRERIFFPLVSG
REGGSGLGLTLAQTFVQQHDGLIECDSRPGLTDFRILVPLH
>Mature_360_residues
PPLPFSHSAPPSYPGLDAVPNPVLFVRHPSLTIFYANPAAEATLAVSRRQLVGTSLHELFADPVELMEMIGTVASRLFDA
KRRDLTLERPGHEALHVHAVVGTIDFEPGCALLELLPNEQKMRSEREERLLDLTSANKELIRNLAHEIKNPLGGIRGAAQ
LLEFELPERALREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVLERVRSVVLAEFPQGLRIVRDYDASLPEFR
GDKEQLIQAVLNIVHNAAHALGPRIAQGNAEIILRTRVARQVTIAKHLYKLALDLHVVDNGPGIPDDIRERIFFPLVSGR
EGGSGLGLTLAQTFVQQHDGLIECDSRPGLTDFRILVPLH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=347, Percent_Identity=44.956772334294, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1788549, Length=337, Percent_Identity=28.1899109792285, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=251, Percent_Identity=27.4900398406374, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40257; Mature: 40126

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLPFSHSAPPSYPGLDAVPNPVLFVRHPSLTIFYANPAAEATLAVSRRQLVGTSLHEL
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
FADPVELMEMIGTVASRLFDAKRRDLTLERPGHEALHVHAVVGTIDFEPGCALLELLPNE
HCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEEEEEEEEECCCCHHHHHHCCCH
QKMRSEREERLLDLTSANKELIRNLAHEIKNPLGGIRGAAQLLEFELPERALREYTQVII
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KESDRLQTLVDRLLEPHRHPHIVGDVNIHEVLERVRSVVLAEFPQGLRIVRDYDASLPEF
CCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
RGDKEQLIQAVLNIVHNAAHALGPRIAQGNAEIILRTRVARQVTIAKHLYKLALDLHVVD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEC
NGPGIPDDIRERIFFPLVSGREGGSGLGLTLAQTFVQQHDGLIECDSRPGLTDFRILVPL
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEC
H
C
>Mature Secondary Structure 
PPLPFSHSAPPSYPGLDAVPNPVLFVRHPSLTIFYANPAAEATLAVSRRQLVGTSLHEL
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
FADPVELMEMIGTVASRLFDAKRRDLTLERPGHEALHVHAVVGTIDFEPGCALLELLPNE
HCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEEEEEEEEECCCCHHHHHHCCCH
QKMRSEREERLLDLTSANKELIRNLAHEIKNPLGGIRGAAQLLEFELPERALREYTQVII
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
KESDRLQTLVDRLLEPHRHPHIVGDVNIHEVLERVRSVVLAEFPQGLRIVRDYDASLPEF
CCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
RGDKEQLIQAVLNIVHNAAHALGPRIAQGNAEIILRTRVARQVTIAKHLYKLALDLHVVD
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEC
NGPGIPDDIRERIFFPLVSGREGGSGLGLTLAQTFVQQHDGLIECDSRPGLTDFRILVPL
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEC
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]