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The map label for this gene is rumA [H]

Identifier: 17545928

GI number: 17545928

Start: 1277668

End: 1279020

Strand: Direct

Name: rumA [H]

Synonym: RSc1209

Alternate gene names: 17545928

Gene position: 1277668-1279020 (Clockwise)

Preceding gene: 17545926

Following gene: 17545930

Centisome position: 34.38

GC content: 67.78

Gene sequence:

>1353_bases
ATGCCCGTGGCGGGGCCGCTGGAGATTGTGTCGCTCGATAACGAAGCGCGCGGCGTGGGCCGTCTGTCCAACGAGGATGG
TACGCCCGGCAAAGTGGTGTTCGTGGAAGGCGCATTGCCGGGTGAGCGGGTGACTTACCGCAGCCATCGGGCCAAGCCGT
CCTATGAGCAGGCCACCCTGGTCGATATTCTGCGCCAGTCGGCCTCGCGCGTGACGCCGCAGTGCAGGTTCTTCGGCACC
TGCGGCGGCTGCTCGATGCAGCATCTGGATTCGCGTGCGCAAATCGCCATCAAGCAGCGCGTGCTGGAAGACGACCTGTG
GCACCTGGCCAAGCTCCGTCCCGATGTGGTGTTCCGCCCCATCGCCGGGCCGGACTGGGGGTATCGCTATCGCGCGCGGC
TGACCGTGCGCCATGTGGCGGCCAAGGGCGGAGTGCTGGTGGGCTTCCACGAGCGCAAGAGCAGCTACGTGGCCGATATG
ACCTCGTGCGAGGTGCTCCCGCCGCACGTGTCCGCATTGCTGGTGCCGTTGCGCGAACTGGTCGGCCGCCTGAGCATCGT
CCAGCGCATGCCGCAGATCGAGGTGGCGGTCGGCCAGGATGTGACCGCGCTGGTGCTGCGTATCCTCGAGCCGCTCACGT
CGGCCGACGAGGCGGAGCTGCGCGCGTTCGCCGACCGGCACGCCGTGCAGTTCTGGCTGCAGCCGAAAGGGCCGGACACG
GTCTATCCGTTCTATCCGCTGGATCGTGCGCTGACCTACACGCTGCCCGAATTCGGCATCACCATGCCCTTCAAGCCGAC
CGATTTCACGCAGGTCAACCACCAGATCAACCGCGTGCTGATGTCGCGCGCACTCAAGCTGCTGGATGCCCGGCCGGGCG
ACCGTCTGCTCGACCTGTTCTGCGGCATCGGCAACTTCACCCTGCCGATGGCGACGCGCGGGTGCGAAGTGATGGGCATC
GAAGGCAGCGAAGCGCTGACGACCCGTGCGCTGGCCAATGCGCAGCACAACGGGCTCGATGGGAAGACCACGTTTGCCTG
CCGCAATCTGTTCGAGGTGAGCGCGGATGACATCGCGGCGCTGGGCCGGTTCGATCGCTGGCTGGTCGATCCGCCGCGCG
AGGGGGCGCTGGCGGTGTCCAAGGCGGTGGCCGAGCTGTCGCAGCGCGGCGGCGAGGCGGCGGCGCTGCTGCCCGGCCGC
ATCGTCTATGTGTCGTGCAATCCGGCCACCCTGGCGCGCGATGCGGGCCTGCTGGTACACGAAGCCGGTTACCGCCTGGC
GGGGGCCGGGGTGGTGAACATGTTCCCGCACACGTCGCACGTCGAATCGATCGCGGTGTTCGAGCGCGCCTGA

Upstream 100 bases:

>100_bases
GCCCGTGTCTCAAGCCGCACCTCCTGCTTTGCCCTCGTCGCCGGTTTCGGCATCTCCCGCGTCGGCCAAGCACTCGCGCG
CATCACGCAGGAAACCCGAG

Downstream 100 bases:

>100_bases
GGCGCGTTCCGGCAAAGAAAAACCGGCGCATCGGGCGCCGGTTTTTTTTTCGTCTGCTGCGCCGGATCCGGCGCAGGCGC
GGCATCAGTTGCGGTTGCCG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPVAGPLEIVSLDNEARGVGRLSNEDGTPGKVVFVEGALPGERVTYRSHRAKPSYEQATLVDILRQSASRVTPQCRFFGT
CGGCSMQHLDSRAQIAIKQRVLEDDLWHLAKLRPDVVFRPIAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADM
TSCEVLPPHVSALLVPLRELVGRLSIVQRMPQIEVAVGQDVTALVLRILEPLTSADEAELRAFADRHAVQFWLQPKGPDT
VYPFYPLDRALTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDARPGDRLLDLFCGIGNFTLPMATRGCEVMGI
EGSEALTTRALANAQHNGLDGKTTFACRNLFEVSADDIAALGRFDRWLVDPPREGALAVSKAVAELSQRGGEAAALLPGR
IVYVSCNPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERA

Sequences:

>Translated_450_residues
MPVAGPLEIVSLDNEARGVGRLSNEDGTPGKVVFVEGALPGERVTYRSHRAKPSYEQATLVDILRQSASRVTPQCRFFGT
CGGCSMQHLDSRAQIAIKQRVLEDDLWHLAKLRPDVVFRPIAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADM
TSCEVLPPHVSALLVPLRELVGRLSIVQRMPQIEVAVGQDVTALVLRILEPLTSADEAELRAFADRHAVQFWLQPKGPDT
VYPFYPLDRALTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDARPGDRLLDLFCGIGNFTLPMATRGCEVMGI
EGSEALTTRALANAQHNGLDGKTTFACRNLFEVSADDIAALGRFDRWLVDPPREGALAVSKAVAELSQRGGEAAALLPGR
IVYVSCNPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERA
>Mature_449_residues
PVAGPLEIVSLDNEARGVGRLSNEDGTPGKVVFVEGALPGERVTYRSHRAKPSYEQATLVDILRQSASRVTPQCRFFGTC
GGCSMQHLDSRAQIAIKQRVLEDDLWHLAKLRPDVVFRPIAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADMT
SCEVLPPHVSALLVPLRELVGRLSIVQRMPQIEVAVGQDVTALVLRILEPLTSADEAELRAFADRHAVQFWLQPKGPDTV
YPFYPLDRALTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDARPGDRLLDLFCGIGNFTLPMATRGCEVMGIE
GSEALTTRALANAQHNGLDGKTTFACRNLFEVSADDIAALGRFDRWLVDPPREGALAVSKAVAELSQRGGEAAALLPGRI
VYVSCNPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=448, Percent_Identity=37.2767857142857, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1787083, Length=405, Percent_Identity=23.4567901234568, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24666579, Length=211, Percent_Identity=31.2796208530806, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49266; Mature: 49135

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAGPLEIVSLDNEARGVGRLSNEDGTPGKVVFVEGALPGERVTYRSHRAKPSYEQATL
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEHHCCCCCCHHHHHH
VDILRQSASRVTPQCRFFGTCGGCSMQHLDSRAQIAIKQRVLEDDLWHLAKLRPDVVFRP
HHHHHHHHHHCCCCEEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
IAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADMTSCEVLPPHVSALLVPLREL
CCCCCCCCEEEEEEEEEEHHCCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHH
VGRLSIVQRMPQIEVAVGQDVTALVLRILEPLTSADEAELRAFADRHAVQFWLQPKGPDT
HHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCCC
VYPFYPLDRALTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDARPGDRLLDLF
CCCCCCCCCHHEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
CGIGNFTLPMATRGCEVMGIEGSEALTTRALANAQHNGLDGKTTFACRNLFEVSADDIAA
HCCCCCEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHH
LGRFDRWLVDPPREGALAVSKAVAELSQRGGEAAALLPGRIVYVSCNPATLARDAGLLVH
HHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHCCCEEEE
EAGYRLAGAGVVNMFPHTSHVESIAVFERA
CCCCEEECCCEEECCCCCHHHHHHHHEECC
>Mature Secondary Structure 
PVAGPLEIVSLDNEARGVGRLSNEDGTPGKVVFVEGALPGERVTYRSHRAKPSYEQATL
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEHHCCCCCCHHHHHH
VDILRQSASRVTPQCRFFGTCGGCSMQHLDSRAQIAIKQRVLEDDLWHLAKLRPDVVFRP
HHHHHHHHHHCCCCEEEEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
IAGPDWGYRYRARLTVRHVAAKGGVLVGFHERKSSYVADMTSCEVLPPHVSALLVPLREL
CCCCCCCCEEEEEEEEEEHHCCCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHH
VGRLSIVQRMPQIEVAVGQDVTALVLRILEPLTSADEAELRAFADRHAVQFWLQPKGPDT
HHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCCC
VYPFYPLDRALTYTLPEFGITMPFKPTDFTQVNHQINRVLMSRALKLLDARPGDRLLDLF
CCCCCCCCCHHEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
CGIGNFTLPMATRGCEVMGIEGSEALTTRALANAQHNGLDGKTTFACRNLFEVSADDIAA
HCCCCCEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHH
LGRFDRWLVDPPREGALAVSKAVAELSQRGGEAAALLPGRIVYVSCNPATLARDAGLLVH
HHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCHHHHHCCCEEEE
EAGYRLAGAGVVNMFPHTSHVESIAVFERA
CCCCEEECCCEEECCCCCHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA