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The map label for this gene is rpoS [H]

Identifier: 17545926

GI number: 17545926

Start: 1275477

End: 1276610

Strand: Direct

Name: rpoS [H]

Synonym: RSc1207

Alternate gene names: 17545926

Gene position: 1275477-1276610 (Clockwise)

Preceding gene: 17545925

Following gene: 17545928

Centisome position: 34.32

GC content: 65.78

Gene sequence:

>1134_bases
ATGCCGCGCCAGAAAGCCGCCACGTCCGCCAACCCCGCCGATGAGACCATCGGCGCCGTCGATGGCCCGAACGCCCCGAA
CGGGCGTAGCGGGCGGCGTGCGCGCGGCGCAGCGCCGGCAGCCTCCGATGACGTCGAAGTCGTCGATCCCCTGATCGATG
AGGCGCCTGCCGCCGAGCCCGCCGACATCGCCGATACCTCGGACGCCAACGACAGCGCCGACGAATCCGACGAAGAGGAC
GAGGACGGCGAGGAGGCTGCGCCTGAGGAAGACTTCCGCACGGTGTTGCAGGCCGAGCTGGCCGCTGACACCGTCCAGCA
TTACCTGAACCGCATCAGCATCAAGCCGCTGCTGACGCCGGCGGAGGAACTGCACTTCTCCACGCTGGCCAAGGCAGGCG
AGTTCGCCGCGCGGCAGGTGATGATCGAGCGCAATCTGCGCCTGGTGGTCAGTATCGCCAAGGGCTATCTGAACCGCGGC
GTGCCGCTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATGCACGCGATCGAGAAATTCGATCCGGAGCGCGGCTT
CCGTTTCTCCACGTATGCCACGTGGTGGATCCGCCAGAGCATCGAGCGGGCCATCATGAACCAGGCACGCACCGTCCGCC
TGCCGGTGCACGTGATCCGCGAGCTCAACCAGGTGCTGCGCGCCAAGCGCCATCTGGAAAAGAGCGGAATCGATGGCCGC
GACGCCAGCATCGAAGACATTGCGCACCTGCTCGGCAAGACCACCGAGGAGGTGCAGGACGTGCTTTCGCTCAACGAGCA
CACGACCTCGCTCGATACGCCGTTCGATCTGGATCCGGGCACTAGCCTGCTCGATTTCCTGTCGGACGAGCATGGCGCCT
CGCCAGACCAGGAAGTCGCGCACCGCGAGTTGTCGCAGCTGATGAAATCCTGGCTGGCACGGCTATCCGACAAGCACCGC
TACGTGGTGGAACGCCGGTTCGGCCTTAACCACATCGAGCCGGCCACCCTCGAAGAGCTGGCCGCCGAGATGGGCCTGAC
GCGCGAGCGTGTGCGTCAGATCCAGCAGGAAGCGCTGGTCAAGCTCAAGCGGCACTTCGCCTCGCAAGGCGTGCGCAAGG
ATGCGGTGCTGTAA

Upstream 100 bases:

>100_bases
GCAGTACCGATGCGGACCGCGTGAAACTTCACTTTGAAGTGCGCCGCAACGGTAAGCCTGTCGATCCGATGCGTTTCCTT
CCGCCGCAATAGAGGAATGC

Downstream 100 bases:

>100_bases
GGCGCCGCCCAGCGCTTAATTCATCCACGTTGTCTATTCCGCGTCGCGCAGCTCCAGCAGCGTTGGCTGGTGGTCGGATG
CCTGCGTCGTGCTGTTGACT

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MPRQKAATSANPADETIGAVDGPNAPNGRSGRRARGAAPAASDDVEVVDPLIDEAPAAEPADIADTSDANDSADESDEED
EDGEEAAPEEDFRTVLQAELAADTVQHYLNRISIKPLLTPAEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNRG
VPLLDLIEEGNLGLMHAIEKFDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGIDGR
DASIEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEVAHRELSQLMKSWLARLSDKHR
YVVERRFGLNHIEPATLEELAAEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL

Sequences:

>Translated_377_residues
MPRQKAATSANPADETIGAVDGPNAPNGRSGRRARGAAPAASDDVEVVDPLIDEAPAAEPADIADTSDANDSADESDEED
EDGEEAAPEEDFRTVLQAELAADTVQHYLNRISIKPLLTPAEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNRG
VPLLDLIEEGNLGLMHAIEKFDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGIDGR
DASIEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEVAHRELSQLMKSWLARLSDKHR
YVVERRFGLNHIEPATLEELAAEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL
>Mature_376_residues
PRQKAATSANPADETIGAVDGPNAPNGRSGRRARGAAPAASDDVEVVDPLIDEAPAAEPADIADTSDANDSADESDEEDE
DGEEAAPEEDFRTVLQAELAADTVQHYLNRISIKPLLTPAEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNRGV
PLLDLIEEGNLGLMHAIEKFDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGIDGRD
ASIEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEVAHRELSQLMKSWLARLSDKHRY
VVERRFGLNHIEPATLEELAAEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=275, Percent_Identity=53.0909090909091, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=40, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789871, Length=278, Percent_Identity=29.8561151079137, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41831; Mature: 41700

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQKAATSANPADETIGAVDGPNAPNGRSGRRARGAAPAASDDVEVVDPLIDEAPAAEP
CCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
ADIADTSDANDSADESDEEDEDGEEAAPEEDFRTVLQAELAADTVQHYLNRISIKPLLTP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHH
FDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGIDGR
HCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
DASIEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEVA
CCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHH
HRELSQLMKSWLARLSDKHRYVVERRFGLNHIEPATLEELAAEMGLTRERVRQIQQEALV
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
KLKRHFASQGVRKDAVL
HHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
PRQKAATSANPADETIGAVDGPNAPNGRSGRRARGAAPAASDDVEVVDPLIDEAPAAEP
CCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
ADIADTSDANDSADESDEEDEDGEEAAPEEDFRTVLQAELAADTVQHYLNRISIKPLLTP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AEELHFSTLAKAGEFAARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHH
FDPERGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKSGIDGR
HCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
DASIEDIAHLLGKTTEEVQDVLSLNEHTTSLDTPFDLDPGTSLLDFLSDEHGASPDQEVA
CCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHH
HRELSQLMKSWLARLSDKHRYVVERRFGLNHIEPATLEELAAEMGLTRERVRQIQQEALV
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
KLKRHFASQGVRKDAVL
HHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]