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The map label for this gene is pyrG

Identifier: 17545845

GI number: 17545845

Start: 1184558

End: 1186222

Strand: Direct

Name: pyrG

Synonym: RSc1126

Alternate gene names: 17545845

Gene position: 1184558-1186222 (Clockwise)

Preceding gene: 17545844

Following gene: 17545846

Centisome position: 31.87

GC content: 65.29

Gene sequence:

>1665_bases
ATGACCAAGTTCGTATTCGTCACCGGTGGCGTTGTGTCCTCGCTCGGCAAGGGCATCGCCGCCGCCTCGCTCGCGGCCAT
CCTTGAGTCGCGCGGCCTCAAAGTCACCCTCCTCAAACTCGATCCCTACATCAACGTCGACCCGGGCACGATGAGCCCGT
TCCAGCACGGCGAGGTGTTCGTGACGGAGGACGGTGCAGAAACCGACCTCGATCTTGGCCACTACGAGCGCTTCGTCTCG
GCCAAGATGCGCAAGGCCAACAACTTCACCACCGGCCAGATCTACGAATCCGTGATCCGCAAGGAGCGTCGCGGCGAGTA
TCTCGGCAAGACGGTGCAGGTCATTCCGCACATCACCAACGAGATCCAGGCCTTCATCGAGCGTGGCGCCAAGGCCTCGC
ACGATGGCAAGGCAGACGTGGCGATCGTCGAGATCGGCGGCACGGTGGGCGACATCGAGTCGCTGCCGTTCCTGGAAGCC
GCGCGGCAGATGAGCCTGCGCCTGGGCCGCAACCAGGCGGCGTTCGTGCATCTGACGCTGGTGCCGTTCATCGCCAGCGC
CGGCGAGCTCAAGACCAAGCCGACCCAGCACTCGGTGCAGAAGCTGCGCGAGATCGGCGTCCAGCCGACCGCGCTGCTGT
GCCGCGCCGACCGCCCCATCCCCGATGACGAGCGCGCGAAGATCTCGCTGTTCGCCAACATGCCGCAGGACGCGGTGATC
TCGGTGTGGGACGTCGACACCATCTACAAGATCCCGCAGATGCTCAACGAGCAGGGCCTCGACCGCATCATCTGCGAGGA
GCTGCGCATCGATGCACCGCCGGCCGACCTGTCCATGTGGTCGCGCATGGTGCACACGCTGGAGAACCCGCAGCACGAGA
TCACCATCGGCATGGTCGGCAAGTATGTCGACCTGACCGAGTCGTACAAGTCGCTGATCGAGGCGCTGCGCCATGCCGGG
CTGCACACCTCGACGCGCGTCAACATCGAGTACATCGATTCCGAAGAGCTGGAGTCGGGCCATACGCAGGTGCTGGAATC
GCTCGACGCGATCCTGGTGCCGGGCGGCTTCGGCAAGCGCGGCACGGAAGGCAAGATCCGCGCGATCCAGTACGCCCGCG
AGAAGACTGTGCCGTACCTGGGCATCTGCCTGGGGATGCAGCTGGCCGTCATCGAGTTCGCCCGCCACGTGGCCGGCATG
CAGGACGCCAACAGCACGGAATTCAACCTCGAGACCGAGCATCCGGTGGTGGCGCTGATCACCGAATGGCAGGATCGCGA
CGGCCGCGTTGAAAAACGCTCGGCCGACTCCGACCTGGGCGGCACCATGCGCCTGGGCGCGCAGCGCGTGCCGGTCCAGA
CCGGCACCAAGGCGTCGCAGATCTATGGTGCTGAGGTCAACGAGCGCCACCGTCATCGCTACGAAGTGAACAACCACTAC
GTGCCGCAGCTGGAGAAGTCCGGGATGATCATCTCGGCGCGCACGCCGTCGGAAAACCTGCCGGAAATGATGGAGCTGCC
GGGCGCGATGCACCCGTGGTTCGTCGGCGTGCAGTTCCACCCGGAATTCACCTCGACACCGCGCGACGGCCACCCGCTGT
TCAAGGCCTATGTGGAAGCCGCGCTGGCGCATCGCCAGCGCCAGGCACAACGCGCGGTCGCCTGA

Upstream 100 bases:

>100_bases
CCATGCGGCACGCCGCCTGAACGGGCGAATTCGGCGGCGCCGACACGGATCGGACAGGGCACCTTTGCTATAATCCGGCT
TTCCCCGGCAAGCCAGGTTT

Downstream 100 bases:

>100_bases
GCGGCAGCGCACGGGAAACACGATGAAACTCTGCGATTTCGAGATCGGCCTCGACCGTCCGTTCTTCCTGATTGCCGGCA
CCTGCGTGATCGAGTCCGAG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVS
AKMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAKASHDGKADVAIVEIGGTVGDIESLPFLEA
ARQMSLRLGRNQAAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVI
SVWDVDTIYKIPQMLNEQGLDRIICEELRIDAPPADLSMWSRMVHTLENPQHEITIGMVGKYVDLTESYKSLIEALRHAG
LHTSTRVNIEYIDSEELESGHTQVLESLDAILVPGGFGKRGTEGKIRAIQYAREKTVPYLGICLGMQLAVIEFARHVAGM
QDANSTEFNLETEHPVVALITEWQDRDGRVEKRSADSDLGGTMRLGAQRVPVQTGTKASQIYGAEVNERHRHRYEVNNHY
VPQLEKSGMIISARTPSENLPEMMELPGAMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAHRQRQAQRAVA

Sequences:

>Translated_554_residues
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVS
AKMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAKASHDGKADVAIVEIGGTVGDIESLPFLEA
ARQMSLRLGRNQAAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVI
SVWDVDTIYKIPQMLNEQGLDRIICEELRIDAPPADLSMWSRMVHTLENPQHEITIGMVGKYVDLTESYKSLIEALRHAG
LHTSTRVNIEYIDSEELESGHTQVLESLDAILVPGGFGKRGTEGKIRAIQYAREKTVPYLGICLGMQLAVIEFARHVAGM
QDANSTEFNLETEHPVVALITEWQDRDGRVEKRSADSDLGGTMRLGAQRVPVQTGTKASQIYGAEVNERHRHRYEVNNHY
VPQLEKSGMIISARTPSENLPEMMELPGAMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAHRQRQAQRAVA
>Mature_553_residues
TKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFVSA
KMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITNEIQAFIERGAKASHDGKADVAIVEIGGTVGDIESLPFLEAA
RQMSLRLGRNQAAFVHLTLVPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVIS
VWDVDTIYKIPQMLNEQGLDRIICEELRIDAPPADLSMWSRMVHTLENPQHEITIGMVGKYVDLTESYKSLIEALRHAGL
HTSTRVNIEYIDSEELESGHTQVLESLDAILVPGGFGKRGTEGKIRAIQYAREKTVPYLGICLGMQLAVIEFARHVAGMQ
DANSTEFNLETEHPVVALITEWQDRDGRVEKRSADSDLGGTMRLGAQRVPVQTGTKASQIYGAEVNERHRHRYEVNNHYV
PQLEKSGMIISARTPSENLPEMMELPGAMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEAALAHRQRQAQRAVA

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=571, Percent_Identity=45.0087565674256, Blast_Score=481, Evalue=1e-136,
Organism=Homo sapiens, GI28559085, Length=571, Percent_Identity=43.6077057793345, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI28559083, Length=571, Percent_Identity=43.6077057793345, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI221316689, Length=571, Percent_Identity=43.6077057793345, Blast_Score=478, Evalue=1e-135,
Organism=Escherichia coli, GI1789142, Length=550, Percent_Identity=61.6363636363636, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=38.752052545156, Blast_Score=422, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=41.4035087719298, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322563, Length=575, Percent_Identity=42.2608695652174, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24664469, Length=571, Percent_Identity=44.1330998248687, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI21357815, Length=516, Percent_Identity=42.8294573643411, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_RALSO (Q8Y0B8)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519247.1
- ProteinModelPortal:   Q8Y0B8
- SMR:   Q8Y0B8
- GeneID:   1219941
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1126
- NMPDR:   fig|267608.1.peg.1126
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   RSOL267608:RSC1126-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 61442; Mature: 61311

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 383-383 ACT_SITE 520-520 ACT_SITE 522-522

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFVSAKMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EIQAFIERGAKASHDGKADVAIVEIGGTVGDIESLPFLEAARQMSLRLGRNQAAFVHLTL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEH
VPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVI
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
SVWDVDTIYKIPQMLNEQGLDRIICEELRIDAPPADLSMWSRMVHTLENPQHEITIGMVG
EEECHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
KYVDLTESYKSLIEALRHAGLHTSTRVNIEYIDSEELESGHTQVLESLDAILVPGGFGKR
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCHHHHHHHHCCEEEECCCCCCC
GTEGKIRAIQYAREKTVPYLGICLGMQLAVIEFARHVAGMQDANSTEFNLETEHPVVALI
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEE
TEWQDRDGRVEKRSADSDLGGTMRLGAQRVPVQTGTKASQIYGAEVNERHRHRYEVNNHY
ECCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCHHHEECCCCCHHHHHHCCCCCCC
VPQLEKSGMIISARTPSENLPEMMELPGAMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEA
CCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
ALAHRQRQAQRAVA
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TKFVFVTGGVVSSLGKGIAAASLAAILESRGLKVTLLKLDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFVSAKMRKANNFTTGQIYESVIRKERRGEYLGKTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EIQAFIERGAKASHDGKADVAIVEIGGTVGDIESLPFLEAARQMSLRLGRNQAAFVHLTL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEH
VPFIASAGELKTKPTQHSVQKLREIGVQPTALLCRADRPIPDDERAKISLFANMPQDAVI
HHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEE
SVWDVDTIYKIPQMLNEQGLDRIICEELRIDAPPADLSMWSRMVHTLENPQHEITIGMVG
EEECHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
KYVDLTESYKSLIEALRHAGLHTSTRVNIEYIDSEELESGHTQVLESLDAILVPGGFGKR
CHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHCCHHHHHHHHCCEEEECCCCCCC
GTEGKIRAIQYAREKTVPYLGICLGMQLAVIEFARHVAGMQDANSTEFNLETEHPVVALI
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEE
TEWQDRDGRVEKRSADSDLGGTMRLGAQRVPVQTGTKASQIYGAEVNERHRHRYEVNNHY
ECCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCCCCCHHHEECCCCCHHHHHHCCCCCCC
VPQLEKSGMIISARTPSENLPEMMELPGAMHPWFVGVQFHPEFTSTPRDGHPLFKAYVEA
CCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
ALAHRQRQAQRAVA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852