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The map label for this gene is purU [H]

Identifier: 17545825

GI number: 17545825

Start: 1163543

End: 1164415

Strand: Reverse

Name: purU [H]

Synonym: RSc1106

Alternate gene names: 17545825

Gene position: 1164415-1163543 (Counterclockwise)

Preceding gene: 17545829

Following gene: 17545817

Centisome position: 31.33

GC content: 63.23

Gene sequence:

>873_bases
ATGGACGCGCTGTCCCGTTCACCATCATTCATTCTGACGCTGTCGTGCCCGAGCGCCCCCGGCCAGGTGGCCGCCGTCGT
CGGCCTGCTTGAGCGCCACCACTGCTACATCGACGAGCTGACCGTTTTCGATGACGACCTGAACCACCGCTTCTTTGTCC
GCTGCGTATTTCATCCGACGGATGCACACGCGCTGCCCGTCGACGTGCTGAGGCAGGAGTTCGCCCCGATTGCGGAAGCC
TTTGACATGCAGTGGGCAATCCACGACGCCCAGGCTCGCCCGAAGGTGCTCATCATGGTGTCGAAGCTCGAGCATTGCCT
CGCGGATCTGCTGTTCCGCTGGCGCATGGGCGAACTGAAGATGGACATCGTCGGCATCGCCTCGAACCACCCTGACCTGG
AACCGCTGGCGCGCCAGCACGACCTGCCGTTCCGCCACTTCCCGATCGCACCGGAAACAAAGGCGCAGCAGGAAGCGCAG
TGGCTGGACCTGTTCGAATCAAGCGGCGCGGAGCTCGTGATCCTCGCGCGCTACATGCAGGTGCTTTCTGCGCAGACGAG
CAAGAAGCTCGTCAATCGCGCCATCAACATTCACCACTCCTTCCTGCCCGGCTTCAAGGGTGCGAAGCCCTATCACCAGG
CCCATGCGCGCGGCGTCAAGCTGATCGGCGCGACCGCGCACTTTGTCACCGACGATCTCGACGAAGGCCCGATCATCGAG
CAGGTGGTCGAGCGGGTCGATCATTCGTACCGGCCTGAACACCTGCTGGCCGTCGGCCGCGACGTCGAATGCCTGACACT
CGCGCGCGCGGTCAAGGCGTTCATTGAACGGCGGGTCTTCCTGAACGCAGACCGGACCGTCGTATTCCAGTGA

Upstream 100 bases:

>100_bases
GGTTCTATCCGAACCATCCTTCGCCCTGCTCTACTGCGCGCATGGCACGCGCGTACGCACGATGCGCCCGCCGGTCGGTC
AACCCCAAAAGAGGAATCGC

Downstream 100 bases:

>100_bases
GTCCAACGCAAGCCGGCCACGATCGCAATGCAACGGTGGCCGACCTTTCCGTGTCGAGTCCCGGATCGGGACGCCGGATC
GCTGCGATTGCGCGTCTTCG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDALSRSPSFILTLSCPSAPGQVAAVVGLLERHHCYIDELTVFDDDLNHRFFVRCVFHPTDAHALPVDVLRQEFAPIAEA
FDMQWAIHDAQARPKVLIMVSKLEHCLADLLFRWRMGELKMDIVGIASNHPDLEPLARQHDLPFRHFPIAPETKAQQEAQ
WLDLFESSGAELVILARYMQVLSAQTSKKLVNRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
QVVERVDHSYRPEHLLAVGRDVECLTLARAVKAFIERRVFLNADRTVVFQ

Sequences:

>Translated_290_residues
MDALSRSPSFILTLSCPSAPGQVAAVVGLLERHHCYIDELTVFDDDLNHRFFVRCVFHPTDAHALPVDVLRQEFAPIAEA
FDMQWAIHDAQARPKVLIMVSKLEHCLADLLFRWRMGELKMDIVGIASNHPDLEPLARQHDLPFRHFPIAPETKAQQEAQ
WLDLFESSGAELVILARYMQVLSAQTSKKLVNRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
QVVERVDHSYRPEHLLAVGRDVECLTLARAVKAFIERRVFLNADRTVVFQ
>Mature_290_residues
MDALSRSPSFILTLSCPSAPGQVAAVVGLLERHHCYIDELTVFDDDLNHRFFVRCVFHPTDAHALPVDVLRQEFAPIAEA
FDMQWAIHDAQARPKVLIMVSKLEHCLADLLFRWRMGELKMDIVGIASNHPDLEPLARQHDLPFRHFPIAPETKAQQEAQ
WLDLFESSGAELVILARYMQVLSAQTSKKLVNRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
QVVERVDHSYRPEHLLAVGRDVECLTLARAVKAFIERRVFLNADRTVVFQ

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=71, Percent_Identity=43.6619718309859, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI209869995, Length=71, Percent_Identity=43.6619718309859, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI209869993, Length=71, Percent_Identity=43.6619718309859, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787483, Length=298, Percent_Identity=36.5771812080537, Blast_Score=191, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17567511, Length=162, Percent_Identity=32.7160493827161, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32799; Mature: 32799

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALSRSPSFILTLSCPSAPGQVAAVVGLLERHHCYIDELTVFDDDLNHRFFVRCVFHPT
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEECCC
DAHALPVDVLRQEFAPIAEAFDMQWAIHDAQARPKVLIMVSKLEHCLADLLFRWRMGELK
CCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEE
MDIVGIASNHPDLEPLARQHDLPFRHFPIAPETKAQQEAQWLDLFESSGAELVILARYMQ
EEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHH
VLSAQTSKKLVNRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHCCCCCCCCHHH
QVVERVDHSYRPEHLLAVGRDVECLTLARAVKAFIERRVFLNADRTVVFQ
HHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MDALSRSPSFILTLSCPSAPGQVAAVVGLLERHHCYIDELTVFDDDLNHRFFVRCVFHPT
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEECCC
DAHALPVDVLRQEFAPIAEAFDMQWAIHDAQARPKVLIMVSKLEHCLADLLFRWRMGELK
CCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEE
MDIVGIASNHPDLEPLARQHDLPFRHFPIAPETKAQQEAQWLDLFESSGAELVILARYMQ
EEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEHHHHHHH
VLSAQTSKKLVNRAINIHHSFLPGFKGAKPYHQAHARGVKLIGATAHFVTDDLDEGPIIE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECHHHHHCCCCCCCCHHH
QVVERVDHSYRPEHLLAVGRDVECLTLARAVKAFIERRVFLNADRTVVFQ
HHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]