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The map label for this gene is yeiE [H]

Identifier: 17545829

GI number: 17545829

Start: 1166672

End: 1167592

Strand: Reverse

Name: yeiE [H]

Synonym: RSc1110

Alternate gene names: 17545829

Gene position: 1167592-1166672 (Counterclockwise)

Preceding gene: 17545831

Following gene: 17545825

Centisome position: 31.42

GC content: 69.49

Gene sequence:

>921_bases
ATGGATAACAAGGTGCCACGCGTCACCCTGCGCCAATGGTCAGTCTTCGTGGCCGTGGCGCGCTGCGAGACCACGACGGC
CGCCGGCGCGCAGCTCAGCCTGTCGCAGTCCGCGGTGAGCGCGGCGCTGGCGGAACTGGAGTCCGCGCTGGCGCAACCGC
TGTTCGACCGGCACGGCCGCCGCCTGCACCTGAACGCGCTCGGACGGCAATTGCTGCCGCAAGCCCAGGCGCTGCTCGAT
CACGCGGATGCGCTGGAGCTCGCCACGCAGCAGCCCAGCGTGCGCCTCAAGCTCGCGGCCAGCAGCACCATCGGCAACTA
CGTGCTGCCCGCCCTGCTCGCGCGCTTGCGCCAGCAGATCGCCCCCGACAGCCAGCTCGACCTGCTGATCGGCAATACGC
AGGACGTGGTCGACGCCGTGCTGCGCTTCGACGTGGACCTGGGCCTGATCGAAGGCTCGTGTCGCGGCGAAGCCCTGGAG
ATCGAGCCCTGGATGGAAGATGAAATGGTGATCGTGGCCGCGCCCGACCACCCTCTCGCGCACGGTCGCGTCTCGCACGC
GGCGCTGCGGCAGGCGGCCTGGCTGATGCGCGAACCCGGCTCGGGCACGCGCGAACTGATCGACAGCCGCATCGCCGCCG
CCGTCGGCCCCCTGCATGTCGCACTCGAACTCGGGCATTCGGAAGCGATCCAGCGCACGGTGGCTGCGGGCTACGGCATC
AGCTGCCTGTCGCGGTACGTGGTGAACGATGCCCTGCGCGACGGGCGGCTGGCCATCGTGGACAGCGGCCTGCCGCGCAT
CGCTCGCCCACTGCTGATCGTGCGCCACCGCGACAAGCATCCGACGCGCGGACTGGCTCAGTTTGTCGAGGCGCTGCACA
GCGATGCCGCGGTTTCGAAAAAAAATCGCACGAAGGGCTAG

Upstream 100 bases:

>100_bases
GTCGTCATCACGCTCGCCTTGCTTATTCGTTATTCGCATAACGATATGCGCACCGTGATAACATGAGAAACGGGTAATTT
TCCTATGAATGACCTGTTTT

Downstream 100 bases:

>100_bases
CGCGATCTGAAAAACCTTGATATAGTCTCATTTCTTCGGGGGCGTTAGCTCAGTTGGTAGAGCAGCGGACTCTTAATCCG
TAGGTCGAGTGTTCGAGTCA

Product: transcriptional regulatory DNA-binding transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MDNKVPRVTLRQWSVFVAVARCETTTAAGAQLSLSQSAVSAALAELESALAQPLFDRHGRRLHLNALGRQLLPQAQALLD
HADALELATQQPSVRLKLAASSTIGNYVLPALLARLRQQIAPDSQLDLLIGNTQDVVDAVLRFDVDLGLIEGSCRGEALE
IEPWMEDEMVIVAAPDHPLAHGRVSHAALRQAAWLMREPGSGTRELIDSRIAAAVGPLHVALELGHSEAIQRTVAAGYGI
SCLSRYVVNDALRDGRLAIVDSGLPRIARPLLIVRHRDKHPTRGLAQFVEALHSDAAVSKKNRTKG

Sequences:

>Translated_306_residues
MDNKVPRVTLRQWSVFVAVARCETTTAAGAQLSLSQSAVSAALAELESALAQPLFDRHGRRLHLNALGRQLLPQAQALLD
HADALELATQQPSVRLKLAASSTIGNYVLPALLARLRQQIAPDSQLDLLIGNTQDVVDAVLRFDVDLGLIEGSCRGEALE
IEPWMEDEMVIVAAPDHPLAHGRVSHAALRQAAWLMREPGSGTRELIDSRIAAAVGPLHVALELGHSEAIQRTVAAGYGI
SCLSRYVVNDALRDGRLAIVDSGLPRIARPLLIVRHRDKHPTRGLAQFVEALHSDAAVSKKNRTKG
>Mature_306_residues
MDNKVPRVTLRQWSVFVAVARCETTTAAGAQLSLSQSAVSAALAELESALAQPLFDRHGRRLHLNALGRQLLPQAQALLD
HADALELATQQPSVRLKLAASSTIGNYVLPALLARLRQQIAPDSQLDLLIGNTQDVVDAVLRFDVDLGLIEGSCRGEALE
IEPWMEDEMVIVAAPDHPLAHGRVSHAALRQAAWLMREPGSGTRELIDSRIAAAVGPLHVALELGHSEAIQRTVAAGYGI
SCLSRYVVNDALRDGRLAIVDSGLPRIARPLLIVRHRDKHPTRGLAQFVEALHSDAAVSKKNRTKG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=283, Percent_Identity=47.7031802120141, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=31.4878892733564, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082132, Length=255, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI145693105, Length=270, Percent_Identity=25.5555555555556, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=25.5892255892256, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=203, Percent_Identity=29.5566502463054, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788887, Length=186, Percent_Identity=29.5698924731183, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=208, Percent_Identity=26.4423076923077, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788296, Length=228, Percent_Identity=28.0701754385965, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33052; Mature: 33052

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKVPRVTLRQWSVFVAVARCETTTAAGAQLSLSQSAVSAALAELESALAQPLFDRHGR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
RLHLNALGRQLLPQAQALLDHADALELATQQPSVRLKLAASSTIGNYVLPALLARLRQQI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
APDSQLDLLIGNTQDVVDAVLRFDVDLGLIEGSCRGEALEIEPWMEDEMVIVAAPDHPLA
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCCEEEEECCCCCCC
HGRVSHAALRQAAWLMREPGSGTRELIDSRIAAAVGPLHVALELGHSEAIQRTVAAGYGI
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCH
SCLSRYVVNDALRDGRLAIVDSGLPRIARPLLIVRHRDKHPTRGLAQFVEALHSDAAVSK
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCC
KNRTKG
CCCCCC
>Mature Secondary Structure
MDNKVPRVTLRQWSVFVAVARCETTTAAGAQLSLSQSAVSAALAELESALAQPLFDRHGR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
RLHLNALGRQLLPQAQALLDHADALELATQQPSVRLKLAASSTIGNYVLPALLARLRQQI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
APDSQLDLLIGNTQDVVDAVLRFDVDLGLIEGSCRGEALEIEPWMEDEMVIVAAPDHPLA
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCCEEEEECCCCCCC
HGRVSHAALRQAAWLMREPGSGTRELIDSRIAAAVGPLHVALELGHSEAIQRTVAAGYGI
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCH
SCLSRYVVNDALRDGRLAIVDSGLPRIARPLLIVRHRDKHPTRGLAQFVEALHSDAAVSK
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCC
KNRTKG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]