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The map label for this gene is purR [H]

Identifier: 17545733

GI number: 17545733

Start: 1067248

End: 1068291

Strand: Reverse

Name: purR [H]

Synonym: RSc1014

Alternate gene names: 17545733

Gene position: 1068291-1067248 (Counterclockwise)

Preceding gene: 17545734

Following gene: 17545732

Centisome position: 28.75

GC content: 69.64

Gene sequence:

>1044_bases
ATGGCCACTATCAAGGACGTTGCCGCATTAGCCGGGGTGTCATTCACCACCGTCTCGCATGTCATCAACAACACACGGCC
GGTCAACTCGGAAACGCGCAAGCGCGTGGAAGAGGCCATCCGCGCCACGCGCTATGTACCCAGCGCCGTAGCACGCTCGC
TCAAGCACCGCACCACGCGCACCATCGGCGTGCTGGTGCCGACCGCCACCAACCCCTACTTCGCCGAACTGGCGCGCGGC
ATCGAAGACGTGTGCGCCGCGGCCGGCTACAGCGTCATCCTGTGCAACTCCGATGACGATCCCCGCAAGCAGCGCGACTA
CCTGCGCGTGCTGATGGAGAAGCGCGTCGACGGCCTCATCGTCAGCAGCGCCGGACCGGACAACGCGCTGCTCGATGCAC
TGGGCGAATCCGCGCTGCCCGTGGTGATGGTGGACCGTCCCACCGAGGGCATCCTGGCCGACCAGGTGCAGATCGACCAT
GAGGAAGGCGCCTACCTGGCGACCCGGCATCTGCTCGAGCTCGGGCACCGCCGCATTGCCTGCATCAGCGGACCGTCGAC
GCTGAGCGTGACCGCCGGGCGGCTGGCGGGCTTTCACCGGGCGCTGCGCGAAGCCGGCGTGCCCGAGCACGCCGCGCGCG
TGGCCGAAGGCGACTTCACCAGCCCCGGCGGCTACCGCGCCGCGCGCGAACTGCTGACCACCGGCGAGACGCCCACCGCC
ATCTTCGCCAGCAACGACCTGATGGGCATCGGCGCGCTGCGTGCGGCCGCCGAGCTGGGCATCGCGGTGCCGAAGGAACT
GTCCATCATCGGCTTCGACGACATCGAGCTGAGCCGCTATGTCTATCCGGCGCTGTCCACGGTGGGCCAGTCGATCCGGC
AACTGGGCGAGACCACGGCCTCAACCCTGCTCGAGCACCTGACCGATCACGCCGCGCGGCATCACCACGAGATCCCCGAC
GCGCCGCGCCGCATCGTGCTGCCGCCGCGCCTGTCGCTGCGCGAGTCGACCGCCGAGCCCGCACGCCCCGCGCGCGCGGC
CTAA

Upstream 100 bases:

>100_bases
ATCACGGGCGCGGTCATCGTCGCGGCCGTGGTCATGGATACCTATCGAACCCGGACCAACCGGGTCACTACACGCCAATA
AAGACACAGGGAGCTTCAAC

Downstream 100 bases:

>100_bases
ACTCCAGTCAACAGAAACCGCAACCCTTTGCCCACCATGGTGGCCAAGCGTTCCGTTCTCTCCTCGGCCGCTGCCGACGT
GCTGGTCGTCGGCAGCCTCA

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MATIKDVAALAGVSFTTVSHVINNTRPVNSETRKRVEEAIRATRYVPSAVARSLKHRTTRTIGVLVPTATNPYFAELARG
IEDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGLIVSSAGPDNALLDALGESALPVVMVDRPTEGILADQVQIDH
EEGAYLATRHLLELGHRRIACISGPSTLSVTAGRLAGFHRALREAGVPEHAARVAEGDFTSPGGYRAARELLTTGETPTA
IFASNDLMGIGALRAAAELGIAVPKELSIIGFDDIELSRYVYPALSTVGQSIRQLGETTASTLLEHLTDHAARHHHEIPD
APRRIVLPPRLSLRESTAEPARPARAA

Sequences:

>Translated_347_residues
MATIKDVAALAGVSFTTVSHVINNTRPVNSETRKRVEEAIRATRYVPSAVARSLKHRTTRTIGVLVPTATNPYFAELARG
IEDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGLIVSSAGPDNALLDALGESALPVVMVDRPTEGILADQVQIDH
EEGAYLATRHLLELGHRRIACISGPSTLSVTAGRLAGFHRALREAGVPEHAARVAEGDFTSPGGYRAARELLTTGETPTA
IFASNDLMGIGALRAAAELGIAVPKELSIIGFDDIELSRYVYPALSTVGQSIRQLGETTASTLLEHLTDHAARHHHEIPD
APRRIVLPPRLSLRESTAEPARPARAA
>Mature_346_residues
ATIKDVAALAGVSFTTVSHVINNTRPVNSETRKRVEEAIRATRYVPSAVARSLKHRTTRTIGVLVPTATNPYFAELARGI
EDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGLIVSSAGPDNALLDALGESALPVVMVDRPTEGILADQVQIDHE
EGAYLATRHLLELGHRRIACISGPSTLSVTAGRLAGFHRALREAGVPEHAARVAEGDFTSPGGYRAARELLTTGETPTAI
FASNDLMGIGALRAAAELGIAVPKELSIIGFDDIELSRYVYPALSTVGQSIRQLGETTASTLLEHLTDHAARHHHEIPDA
PRRIVLPPRLSLRESTAEPARPARAA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=348, Percent_Identity=42.816091954023, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1790194, Length=312, Percent_Identity=41.025641025641, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=36.4820846905537, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=37.5, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788474, Length=288, Percent_Identity=37.8472222222222, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=32.258064516129, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789068, Length=294, Percent_Identity=31.9727891156463, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1787580, Length=309, Percent_Identity=30.42071197411, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787906, Length=347, Percent_Identity=28.8184438040346, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI48994940, Length=306, Percent_Identity=29.4117647058824, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790715, Length=325, Percent_Identity=26.1538461538462, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789456, Length=349, Percent_Identity=28.080229226361, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786268, Length=310, Percent_Identity=24.8387096774194, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790689, Length=341, Percent_Identity=25.5131964809384, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790192, Length=219, Percent_Identity=26.9406392694064, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37289; Mature: 37158

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAALAGVSFTTVSHVINNTRPVNSETRKRVEEAIRATRYVPSAVARSLKHRTTR
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
TIGVLVPTATNPYFAELARGIEDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGLI
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
VSSAGPDNALLDALGESALPVVMVDRPTEGILADQVQIDHEEGAYLATRHLLELGHRRIA
EECCCCCHHHHHHCCCCCCCEEEECCCCCCCCHHHEEECCCCCHHHHHHHHHHHCCCEEE
CISGPSTLSVTAGRLAGFHRALREAGVPEHAARVAEGDFTSPGGYRAARELLTTGETPTA
EECCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCE
IFASNDLMGIGALRAAAELGIAVPKELSIIGFDDIELSRYVYPALSTVGQSIRQLGETTA
EEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
STLLEHLTDHAARHHHEIPDAPRRIVLPPRLSLRESTAEPARPARAA
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAALAGVSFTTVSHVINNTRPVNSETRKRVEEAIRATRYVPSAVARSLKHRTTR
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
TIGVLVPTATNPYFAELARGIEDVCAAAGYSVILCNSDDDPRKQRDYLRVLMEKRVDGLI
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
VSSAGPDNALLDALGESALPVVMVDRPTEGILADQVQIDHEEGAYLATRHLLELGHRRIA
EECCCCCHHHHHHCCCCCCCEEEECCCCCCCCHHHEEECCCCCHHHHHHHHHHHCCCEEE
CISGPSTLSVTAGRLAGFHRALREAGVPEHAARVAEGDFTSPGGYRAARELLTTGETPTA
EECCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCE
IFASNDLMGIGALRAAAELGIAVPKELSIIGFDDIELSRYVYPALSTVGQSIRQLGETTA
EEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
STLLEHLTDHAARHHHEIPDAPRRIVLPPRLSLRESTAEPARPARAA
HHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA