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The map label for this gene is rfbD [H]

Identifier: 17545402

GI number: 17545402

Start: 731195

End: 732112

Strand: Direct

Name: rfbD [H]

Synonym: RSc0683

Alternate gene names: 17545402

Gene position: 731195-732112 (Clockwise)

Preceding gene: 17545401

Following gene: 17545403

Centisome position: 19.67

GC content: 69.93

Gene sequence:

>918_bases
ATGCAGCATAAGGCGCACCCTGTTCCGAAGCTGCTGGTCACGGGAAGCAACGGCCAGGTCGGCTTTGAGCTGCGCCGCAG
CCTGGCGCCCCTGGGCGAGGTGATCGCGCTGGACCGGGCCGCCTGCGACCTGACCCGGCCCGATGCGCTGCGCCGGCTGG
TGCGCGAGCACCGCCCCGACGTGATCGTCAACCCGGCGGCCTACACTGCTGTCGACAAGGCCGAGACCGATGCCGAAACG
GCATTCGCCGTCAACGGCATGGCGGCCGGTGTCCTGGCCGAGGAAGCCCGCGCGCTGGGCAGCCTGCTGGTGCACTACAG
CACCGATTACGTTTTCGACGGCAGCAAGGCCGCCGCCTACGTCGAGACCGATGCGGTCAACCCGCAGTCCGTCTACGGCA
AGAGCAAGCTCGCGGGCGAGCAGGCGATCGCCGCCTCGGGCGCGGCGGCGCTGGTGCTGCGTACCTGCTGGGTGGCCGGC
GCGCACGGCGGCAATTTCGCCAAGACGATGCTCAAGCTGGGCCGCGAGCGGGACAGCCTGCGCGTGATCGCCGACCAGTT
CGGCGCGCCCACCACGGCCGCGCTGATCGCCGACGTGACCGCGCAGATCGTCGCGCGCTTCTGGCTGTCGGGCGATCGCG
CCGCATTTCCGGGCGGCCTGTATCACCTGGCCGCGGCGGGCGAGACGACCTGGCACGGCTATGCCACGGCCGTGCTGCGC
TATGCCAAGGTGCGCGGACTGGAGCTGAAGGTCGACCCCGAGCGCATCGAAGCCATTCCGGCGACGGCGTATCCGCTGCC
CGCGCCGCGGCCCGCCAACTCCCGCATGGACACCGGCAAGCTCACTGAAACGTTCGGCATCCACCTGCCGGACTGGCAGC
AGGGTGTCCATCTCCTGCTGGACCAGATCTTTTCCTGA

Upstream 100 bases:

>100_bases
GAGACCGGCCTGCGCAAGACCGTCCAGTGGTACCTGGACAATCAGGTGTGGGTGCAGGACGTGGTCTCGGGCGAATACCG
GAACTGGGTCGCGAAGCAGT

Downstream 100 bases:

>100_bases
ACGAATCGCCATGCGTAAAGGCATTATCCTGGCCGGAGGCTCCGGCACGCGACTCTATCCGATCACCCGCTCGGTTTCGA
AGCAGCTGCTGCCGGTGTAC

Product: dTDP-4-dehydrorhamnose reductase oxidoreductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MQHKAHPVPKLLVTGSNGQVGFELRRSLAPLGEVIALDRAACDLTRPDALRRLVREHRPDVIVNPAAYTAVDKAETDAET
AFAVNGMAAGVLAEEARALGSLLVHYSTDYVFDGSKAAAYVETDAVNPQSVYGKSKLAGEQAIAASGAAALVLRTCWVAG
AHGGNFAKTMLKLGRERDSLRVIADQFGAPTTAALIADVTAQIVARFWLSGDRAAFPGGLYHLAAAGETTWHGYATAVLR
YAKVRGLELKVDPERIEAIPATAYPLPAPRPANSRMDTGKLTETFGIHLPDWQQGVHLLLDQIFS

Sequences:

>Translated_305_residues
MQHKAHPVPKLLVTGSNGQVGFELRRSLAPLGEVIALDRAACDLTRPDALRRLVREHRPDVIVNPAAYTAVDKAETDAET
AFAVNGMAAGVLAEEARALGSLLVHYSTDYVFDGSKAAAYVETDAVNPQSVYGKSKLAGEQAIAASGAAALVLRTCWVAG
AHGGNFAKTMLKLGRERDSLRVIADQFGAPTTAALIADVTAQIVARFWLSGDRAAFPGGLYHLAAAGETTWHGYATAVLR
YAKVRGLELKVDPERIEAIPATAYPLPAPRPANSRMDTGKLTETFGIHLPDWQQGVHLLLDQIFS
>Mature_305_residues
MQHKAHPVPKLLVTGSNGQVGFELRRSLAPLGEVIALDRAACDLTRPDALRRLVREHRPDVIVNPAAYTAVDKAETDAET
AFAVNGMAAGVLAEEARALGSLLVHYSTDYVFDGSKAAAYVETDAVNPQSVYGKSKLAGEQAIAASGAAALVLRTCWVAG
AHGGNFAKTMLKLGRERDSLRVIADQFGAPTTAALIADVTAQIVARFWLSGDRAAFPGGLYHLAAAGETTWHGYATAVLR
YAKVRGLELKVDPERIEAIPATAYPLPAPRPANSRMDTGKLTETFGIHLPDWQQGVHLLLDQIFS

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=302, Percent_Identity=29.4701986754967, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI11034825, Length=302, Percent_Identity=29.4701986754967, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788352, Length=300, Percent_Identity=47.6666666666667, Blast_Score=274, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32560; Mature: 32560

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHKAHPVPKLLVTGSNGQVGFELRRSLAPLGEVIALDRAACDLTRPDALRRLVREHRPD
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
VIVNPAAYTAVDKAETDAETAFAVNGMAAGVLAEEARALGSLLVHYSTDYVFDGSKAAAY
EEECCHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEE
VETDAVNPQSVYGKSKLAGEQAIAASGAAALVLRTCWVAGAHGGNFAKTMLKLGRERDSL
EEECCCCHHHHCCCHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHH
RVIADQFGAPTTAALIADVTAQIVARFWLSGDRAAFPGGLYHLAAAGETTWHGYATAVLR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
YAKVRGLELKVDPERIEAIPATAYPLPAPRPANSRMDTGKLTETFGIHLPDWQQGVHLLL
HHHHCCCEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
DQIFS
HHHCC
>Mature Secondary Structure
MQHKAHPVPKLLVTGSNGQVGFELRRSLAPLGEVIALDRAACDLTRPDALRRLVREHRPD
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
VIVNPAAYTAVDKAETDAETAFAVNGMAAGVLAEEARALGSLLVHYSTDYVFDGSKAAAY
EEECCHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEE
VETDAVNPQSVYGKSKLAGEQAIAASGAAALVLRTCWVAGAHGGNFAKTMLKLGRERDSL
EEECCCCHHHHCCCHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHH
RVIADQFGAPTTAALIADVTAQIVARFWLSGDRAAFPGGLYHLAAAGETTWHGYATAVLR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
YAKVRGLELKVDPERIEAIPATAYPLPAPRPANSRMDTGKLTETFGIHLPDWQQGVHLLL
HHHHCCCEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHH
DQIFS
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]