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The map label for this gene is rfbB [H]

Identifier: 17545401

GI number: 17545401

Start: 730141

End: 731205

Strand: Direct

Name: rfbB [H]

Synonym: RSc0682

Alternate gene names: 17545401

Gene position: 730141-731205 (Clockwise)

Preceding gene: 17545399

Following gene: 17545402

Centisome position: 19.65

GC content: 64.6

Gene sequence:

>1065_bases
TTGTCCCATATCCTCGTAACCGGTGGCGCCGGCTTCATCGGCGGCAACTTTGTCCTGAACTGGCTGGCCAATCCTGGCGC
GGACGGCGTTGTCAACGTCGATAAGCTGACCTACGCCGGCAATCGCAAGACGCTGGCCGCCATCGAGCATGACCCGCGCC
ACATCTTCTCCCAGACGGATATCTGCGACCGTGCGGCGCTCGACAGCCTGTTCGCCCAGCACAAGCCGCGCGCGGTGGTC
CACTTCGCCGCGGAGAGCCACGTGGATCGCTCCATCCACGGCCCGGCCGAATTCATCCAGACCAACATCGTCGGCACCTT
CACGCTGCTGGAAGCCGTGCGCGCCTACTGGGGCGGGCTCGATGCCGACGCCAAGGCCGCGTTCCGCTTCCTGCACGTCT
CGACCGACGAGGTTTTCGGCTCGCTCAGCGCCACCGATCCGCAATTCTCGGAAACCACGCCGTACGCGCCCAACAGCCCG
TACTCGGCATCCAAGGCCGCCTCGGATCACCTGGTGCGCGCTTATCACCACACCTACGGGCTGCCGGTCCTGACGACCAA
CTGCTCCAACAACTACGGCCCCTACCATTTCCCGGAAAAGCTGATTCCGCTGATGATCACCAATGCACTCAGCGGCAAGC
CGCTGCCGGTGTACGGTGATGGGCAGAACGTGCGCGACTGGCTCTACGTGGGCGACCACTGCGCCGCCATCCGCGAGGTG
CTTGCGCGTGGCCGTCTGGGCGAGACCTACAACGTCGGCGGCTGGAACGAGAAGACCAACCTCGAGGTCGTGTACACGCT
GTGCGACCTGCTCGACGAACTGAAGCCGAAGGCAACGGGCTCCTACCGCGACCAGATCGCGTTCGTGAAAGACCGGCCGG
GCCACGACCGCCGCTACGCCATCGACGCCCGCAAGCTCGAGCGCGAACTCGGCTGGAAACCCGCCGAGACCTTCGAGACC
GGCCTGCGCAAGACCGTCCAGTGGTACCTGGACAATCAGGTGTGGGTGCAGGACGTGGTCTCGGGCGAATACCGGAACTG
GGTCGCGAAGCAGTATGCAGCATAA

Upstream 100 bases:

>100_bases
AATACGCCACTTCTCAACATACTCAACGATTCCGCCGCCCTCGCGGGCGTACCGAGCGGACCAGGCGCGTGCCAACGGCG
CGCGACAAGGAGCAGAGCGT

Downstream 100 bases:

>100_bases
GGCGCACCCTGTTCCGAAGCTGCTGGTCACGGGAAGCAACGGCCAGGTCGGCTTTGAGCTGCGCCGCAGCCTGGCGCCCC
TGGGCGAGGTGATCGCGCTG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSHILVTGGAGFIGGNFVLNWLANPGADGVVNVDKLTYAGNRKTLAAIEHDPRHIFSQTDICDRAALDSLFAQHKPRAVV
HFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWGGLDADAKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSP
YSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCAAIREV
LARGRLGETYNVGGWNEKTNLEVVYTLCDLLDELKPKATGSYRDQIAFVKDRPGHDRRYAIDARKLERELGWKPAETFET
GLRKTVQWYLDNQVWVQDVVSGEYRNWVAKQYAA

Sequences:

>Translated_354_residues
MSHILVTGGAGFIGGNFVLNWLANPGADGVVNVDKLTYAGNRKTLAAIEHDPRHIFSQTDICDRAALDSLFAQHKPRAVV
HFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWGGLDADAKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSP
YSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCAAIREV
LARGRLGETYNVGGWNEKTNLEVVYTLCDLLDELKPKATGSYRDQIAFVKDRPGHDRRYAIDARKLERELGWKPAETFET
GLRKTVQWYLDNQVWVQDVVSGEYRNWVAKQYAA
>Mature_353_residues
SHILVTGGAGFIGGNFVLNWLANPGADGVVNVDKLTYAGNRKTLAAIEHDPRHIFSQTDICDRAALDSLFAQHKPRAVVH
FAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWGGLDADAKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPY
SASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCAAIREVL
ARGRLGETYNVGGWNEKTNLEVVYTLCDLLDELKPKATGSYRDQIAFVKDRPGHDRRYAIDARKLERELGWKPAETFETG
LRKTVQWYLDNQVWVQDVVSGEYRNWVAKQYAA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=35.258358662614, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI42516563, Length=332, Percent_Identity=25.6024096385542, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI56237023, Length=361, Percent_Identity=25.4847645429363, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI56118217, Length=361, Percent_Identity=25.4847645429363, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI189083684, Length=361, Percent_Identity=25.4847645429363, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4504031, Length=284, Percent_Identity=26.056338028169, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=63.3053221288515, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=64.6551724137931, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=28.7749287749288, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788366, Length=364, Percent_Identity=25, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788365, Length=260, Percent_Identity=23.4615384615385, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=37.5739644970414, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=34.7432024169184, Blast_Score=190, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI71982035, Length=353, Percent_Identity=27.7620396600567, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=355, Percent_Identity=27.6056338028169, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=23.3532934131737, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=282, Percent_Identity=24.468085106383, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901786, Length=284, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=284, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=284, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=284, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=284, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=367, Percent_Identity=26.9754768392371, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19923002, Length=359, Percent_Identity=28.9693593314763, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=25.9036144578313, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24158427, Length=275, Percent_Identity=26.9090909090909, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39385; Mature: 39253

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHILVTGGAGFIGGNFVLNWLANPGADGVVNVDKLTYAGNRKTLAAIEHDPRHIFSQTD
CCEEEEECCCCEECCHHHEEECCCCCCCCEEEHHHEEECCCCCEEEEECCCHHHHHCCCC
ICDRAALDSLFAQHKPRAVVHFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWGGL
HHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DADAKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSASKAASDHLVRAYHHTYG
CCCHHHHHHEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCAAIREV
CCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCHHEEECHHHHHHHHH
LARGRLGETYNVGGWNEKTNLEVVYTLCDLLDELKPKATGSYRDQIAFVKDRPGHDRRYA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCEEEEECCCCCCCEEE
IDARKLERELGWKPAETFETGLRKTVQWYLDNQVWVQDVVSGEYRNWVAKQYAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SHILVTGGAGFIGGNFVLNWLANPGADGVVNVDKLTYAGNRKTLAAIEHDPRHIFSQTD
CEEEEECCCCEECCHHHEEECCCCCCCCEEEHHHEEECCCCCEEEEECCCHHHHHCCCC
ICDRAALDSLFAQHKPRAVVHFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWGGL
HHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DADAKAAFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSASKAASDHLVRAYHHTYG
CCCHHHHHHEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCAAIREV
CCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCHHEEECHHHHHHHHH
LARGRLGETYNVGGWNEKTNLEVVYTLCDLLDELKPKATGSYRDQIAFVKDRPGHDRRYA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCEEEEECCCCCCCEEE
IDARKLERELGWKPAETFETGLRKTVQWYLDNQVWVQDVVSGEYRNWVAKQYAA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]