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The map label for this gene is dusB [H]

Identifier: 17545225

GI number: 17545225

Start: 539781

End: 540851

Strand: Reverse

Name: dusB [H]

Synonym: RSc0506

Alternate gene names: 17545225

Gene position: 540851-539781 (Counterclockwise)

Preceding gene: 17545226

Following gene: 17545224

Centisome position: 14.55

GC content: 69.65

Gene sequence:

>1071_bases
TTGCAGATCGGACCGCATATCCTGCGCAACAACCTGTTCGTCGCCCCCATGGCGGGCGTGACGGACCGCCCGTTCCGCCA
GCTGTGCAAGCGGCTGGGCGCCGGCTATGCGGTCTCGGAGATGGTGGCGTCCAACGCGCAGCTGTGGAAGAGCGAGAGGA
CCATGCGCCGCGCCAACCACACCGGCGAGGTCGAGCCGATCGCCGTGCAGATCGCCGGCGCCGAGCCGATGATGATGGCC
GAGGCCGCGCGCTACAACGTCGAGCGCGGCGCGCAGATCATCGACATCAACATGGGCTGCCCGGCCAAGAAGGTGTGCAA
CGTGGCGGCCGGCTCGGCCCTGCTGCAGAACGAGCCGCTGGTGGCGCGCATCGTCGAGGCGGTGGTCGGCGCGGTGGGCG
ACCGCGTGCCGGTCACGCTCAAGATCCGCACCGGCTGGGACCGCGAGCACCGCAACGCGCTGACGGTCGCGCGCATCGCG
CAGGAAGCCGGCATCAGCATGCTGACCGTGCACGGCCGCACCCGCGCCGACTTGTACCACGGCGAGGCCGAGTACGAGAC
CATCGCCGCGGTCAAGGCCTCGGTGCGCATCCCGGTGGTCGCCAACGGCGACATCGGCACGCCGGCCAAGGCCCGGCACG
TGCTGGAGGTGACCGGCGCCGACGCCATCATGATCGGCCGCGCCGCCCAGGGGCGCCCGTGGCTGTTCCGCGAGATCGAG
CACTTCCTGAAGACCGGCACGCTGCTGCCGGCGCCGGAGGTCGAAGAGATCCGCGGCATCATGAACACGCACCTGGAAGA
GCATTACGCGTTCTACGGCGAGTACACCGGCGTGCGCACCGCGCGCAAGCACATCGCCTGGTACACGCGGGGGCTGGCGG
GCGCCAACCTGTTCCGCCACCGCATGAACACCATCGAAGACACCGCGGCCCAGCTGGCCGCCGTCAATGCCTTCTTCGAC
GAGCAGCGCGCGCTGTCCGACCGCCTGGTCTACACGGACCAGGACGATGCCGCGGGCGGCCCTCCCACGACGGGCAACAA
CAACGACAACAAGGAACTGCTTGCCGCATGA

Upstream 100 bases:

>100_bases
TAAAATCCCGTCCTCGCATTTTTACTCCCCGTCGTTCCGCCTGCCCGGGCGGAAGGGGCCGCTTTTTCGTTTCTTGGTCT
TCGCTTCTTCGGATCCCGCC

Downstream 100 bases:

>100_bases
GCCGCAACCCCATCGAACGCTGCATCCGGGACAGCCTGGATACGTACTACCGCGACCTGGACGGCGAGAACCCGTCCAAC
GTCTACGACATGGTGCTGCA

Product: NIFR3-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSERTMRRANHTGEVEPIAVQIAGAEPMMMA
EAARYNVERGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVARIVEAVVGAVGDRVPVTLKIRTGWDREHRNALTVARIA
QEAGISMLTVHGRTRADLYHGEAEYETIAAVKASVRIPVVANGDIGTPAKARHVLEVTGADAIMIGRAAQGRPWLFREIE
HFLKTGTLLPAPEVEEIRGIMNTHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRHRMNTIEDTAAQLAAVNAFFD
EQRALSDRLVYTDQDDAAGGPPTTGNNNDNKELLAA

Sequences:

>Translated_356_residues
MQIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSERTMRRANHTGEVEPIAVQIAGAEPMMMA
EAARYNVERGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVARIVEAVVGAVGDRVPVTLKIRTGWDREHRNALTVARIA
QEAGISMLTVHGRTRADLYHGEAEYETIAAVKASVRIPVVANGDIGTPAKARHVLEVTGADAIMIGRAAQGRPWLFREIE
HFLKTGTLLPAPEVEEIRGIMNTHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRHRMNTIEDTAAQLAAVNAFFD
EQRALSDRLVYTDQDDAAGGPPTTGNNNDNKELLAA
>Mature_356_residues
MQIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSERTMRRANHTGEVEPIAVQIAGAEPMMMA
EAARYNVERGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVARIVEAVVGAVGDRVPVTLKIRTGWDREHRNALTVARIA
QEAGISMLTVHGRTRADLYHGEAEYETIAAVKASVRIPVVANGDIGTPAKARHVLEVTGADAIMIGRAAQGRPWLFREIE
HFLKTGTLLPAPEVEEIRGIMNTHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRHRMNTIEDTAAQLAAVNAFFD
EQRALSDRLVYTDQDDAAGGPPTTGNNNDNKELLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=283, Percent_Identity=30.3886925795053, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI239788462, Length=239, Percent_Identity=32.6359832635983, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=31.4159292035398, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=31.7391304347826, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI8923374, Length=280, Percent_Identity=28.2142857142857, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=57.1875, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=251, Percent_Identity=32.2709163346614, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI145693211, Length=303, Percent_Identity=25.7425742574257, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=33.4821428571429, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=231, Percent_Identity=32.4675324675325, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=249, Percent_Identity=27.710843373494, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=273, Percent_Identity=29.6703296703297, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=244, Percent_Identity=30.7377049180328, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=27.7533039647577, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585320, Length=290, Percent_Identity=29.3103448275862, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24580595, Length=251, Percent_Identity=29.4820717131474, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19920448, Length=251, Percent_Identity=29.4820717131474, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19921524, Length=227, Percent_Identity=28.1938325991189, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=30.6451612903226, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38894; Mature: 38894

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSERTMRRANH
CCCCHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
TGEVEPIAVQIAGAEPMMMAEAARYNVERGAQIIDINMGCPAKKVCNVAAGSALLQNEPL
CCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHCCCCH
VARIVEAVVGAVGDRVPVTLKIRTGWDREHRNALTVARIAQEAGISMLTVHGRTRADLYH
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCC
GEAEYETIAAVKASVRIPVVANGDIGTPAKARHVLEVTGADAIMIGRAAQGRPWLFREIE
CCCHHHHHHHHHHEEEEEEEECCCCCCCHHHCEEEEECCCCEEEEECCCCCCCHHHHHHH
HFLKTGTLLPAPEVEEIRGIMNTHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHH
RMNTIEDTAAQLAAVNAFFDEQRALSDRLVYTDQDDAAGGPPTTGNNNDNKELLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIGPHILRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSERTMRRANH
CCCCHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
TGEVEPIAVQIAGAEPMMMAEAARYNVERGAQIIDINMGCPAKKVCNVAAGSALLQNEPL
CCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCHHHCCCCH
VARIVEAVVGAVGDRVPVTLKIRTGWDREHRNALTVARIAQEAGISMLTVHGRTRADLYH
HHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCC
GEAEYETIAAVKASVRIPVVANGDIGTPAKARHVLEVTGADAIMIGRAAQGRPWLFREIE
CCCHHHHHHHHHHEEEEEEEECCCCCCCHHHCEEEEECCCCEEEEECCCCCCCHHHHHHH
HFLKTGTLLPAPEVEEIRGIMNTHLEEHYAFYGEYTGVRTARKHIAWYTRGLAGANLFRH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHH
RMNTIEDTAAQLAAVNAFFDEQRALSDRLVYTDQDDAAGGPPTTGNNNDNKELLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]