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The map label for this gene is purH

Identifier: 17545223

GI number: 17545223

Start: 537887

End: 539461

Strand: Reverse

Name: purH

Synonym: RSc0504

Alternate gene names: 17545223

Gene position: 539461-537887 (Counterclockwise)

Preceding gene: 17545224

Following gene: 17545222

Centisome position: 14.52

GC content: 66.98

Gene sequence:

>1575_bases
ATGATCCAGCAAGCTCTGCTTTCCGTTTCCGACAAGACCGGCATCGTCGACTTTGCCCGCGCGCTGCACGGCGCGGGCGT
CAAGCTCCTCTCCACCGGCGGCACCGCCAAGCTGCTCGCCGAGTCCGGCCTGCCCGTGACGGAAGTGGCCGACTACACCG
GCTTCCCGGAAATGCTCGACGGCCGCGTCAAGACCTTGCACCCGAAGGTGCACGGCGGCATCCTCGCCCGCCGCGACCTG
CCCGAACACATGGCGGCGCTGTCCCAGCACGGCATCCCGACCATCGACCTGCTGGTGGTGAACCTGTATCCGTTCCAGCA
GACCGTTGCCAAGGATGAATGCACGCTGGCCGATGCCATCGAGAACATCGACATCGGCGGCCCGACCATGCTGCGCTCGG
CCGCCAAGAACCACCGCGACGTGACGGTGATCGTCGATCCGGCCGACTACGCCACCGTGCTGGCCGAGATGCAGGCCAAC
GGCAACACGGTCGGCTACGACACCAACTTCATGCTGGCCAAGAAAGTGTTCGCGCACACCGCGCAGTACGACGGCGCCAT
CACCAACTACCTGACCAGCCTGGGACAGGACAAGTCGCACAGCACCCGCAGCCAGTATCCGCAGACGCTGAACCTGGCCT
TCGAGCAGGTGCAGGAGATGCGCTACGGCGAGAACCCGCACCAGTCCGCCGCCTTCTACCGCGACCTGAAGGCCGTGGAC
GGCGCGCTGGCCAACTACGCGCAGCTGCAGGGCAAGGAGCTGTCGTACAACAACATCGCCGATGCCGATGCGGCGTGGGA
GTGCGTGAAATCGTTCGACCCGGCCAAGGGCGCGGCGTGCGTCATCATCAAGCACGCCAACCCGTGCGGCGTGGCCATCG
GCGGCACCGCGCAGGAAGCCTACGAGAAGGCCTTCAAGACCGACTCGACCTCGGCCTTCGGCGGCATCATCGCCTTTAAC
GTGCCGCTGGACGAAGCGGCCGCGCAGGTGGTGGCCAAGCAGTTCGTCGAAGTGCTGATCGCACCGAGCTTCTCCGCGGG
CGCGCGCACGGTATTCGCGGCCAAGCAGAACGTGCGCCTGCTGGAAATTCCGCTGGGCAAGGGCGTCAACGCCTACGACT
TCAAGCGCGTCGGCGGCGGCCTGCTGGTGCAGAGCCCGGATGCCAAGAACGTGCAGTCGGCCGAACTGCGCGTGGTCACC
AAGCGCCACCCGACCCCGAAGGAGATGGACGACCTGCTGTTCGCCTGGCGCGTCGCCAAGTTCGTCAAGTCCAACGCCAT
CGTGTTCTGCGGCGGCGGCATGACGCTGGGCGTGGGCGCCGGCCAGATGAGCCGCGTGGACTCGGCCCGCATCGCCAGCA
TCAAGGCGCAGAACGCGGGCCTGATGCTGTCGGGCTCGGCGGTGGCATCGGACGCCTTCTTCCCGTTCCGCGACGGCCTG
GACGTGGTGGTCGACGCCGGCGCCTCGTGCGTGATCCAGCCGGGCGGCTCGGTGCGCGATGACGAAGTGATCGCCGCCGC
CGACGAGCGCAACGTGGCCATGATCTTCACCGGCACGCGCCACTTCCGCCACTAA

Upstream 100 bases:

>100_bases
GACTGCTCTGAGGCGGCCCGGCACCGTGCCCCGCCGCATCCCGATGTTCCTTTCCTGACGGCGCCTCGCCGCGCCCCATC
CGCCTGACGGCGTTTCCACC

Downstream 100 bases:

>100_bases
GCCACGAAGCATCACCCGCGGCTCCACCGCGCAACGGGGCAAGGCGTAGACTGTCGCCTGCCCCGTTTTTCTTTTGCCTG
TCCCCTTCATGCGCATCCTC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MIQQALLSVSDKTGIVDFARALHGAGVKLLSTGGTAKLLAESGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALSQHGIPTIDLLVVNLYPFQQTVAKDECTLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN
GNTVGYDTNFMLAKKVFAHTAQYDGAITNYLTSLGQDKSHSTRSQYPQTLNLAFEQVQEMRYGENPHQSAAFYRDLKAVD
GALANYAQLQGKELSYNNIADADAAWECVKSFDPAKGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN
VPLDEAAAQVVAKQFVEVLIAPSFSAGARTVFAAKQNVRLLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQSAELRVVT
KRHPTPKEMDDLLFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLMLSGSAVASDAFFPFRDGL
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH

Sequences:

>Translated_524_residues
MIQQALLSVSDKTGIVDFARALHGAGVKLLSTGGTAKLLAESGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALSQHGIPTIDLLVVNLYPFQQTVAKDECTLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN
GNTVGYDTNFMLAKKVFAHTAQYDGAITNYLTSLGQDKSHSTRSQYPQTLNLAFEQVQEMRYGENPHQSAAFYRDLKAVD
GALANYAQLQGKELSYNNIADADAAWECVKSFDPAKGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN
VPLDEAAAQVVAKQFVEVLIAPSFSAGARTVFAAKQNVRLLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQSAELRVVT
KRHPTPKEMDDLLFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLMLSGSAVASDAFFPFRDGL
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH
>Mature_524_residues
MIQQALLSVSDKTGIVDFARALHGAGVKLLSTGGTAKLLAESGLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL
PEHMAALSQHGIPTIDLLVVNLYPFQQTVAKDECTLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN
GNTVGYDTNFMLAKKVFAHTAQYDGAITNYLTSLGQDKSHSTRSQYPQTLNLAFEQVQEMRYGENPHQSAAFYRDLKAVD
GALANYAQLQGKELSYNNIADADAAWECVKSFDPAKGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN
VPLDEAAAQVVAKQFVEVLIAPSFSAGARTVFAAKQNVRLLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQSAELRVVT
KRHPTPKEMDDLLFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLMLSGSAVASDAFFPFRDGL
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=481, Percent_Identity=36.7983367983368, Blast_Score=269, Evalue=4e-72,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=61.0169491525424, Blast_Score=637, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=616, Percent_Identity=32.6298701298701, Blast_Score=274, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI71985574, Length=321, Percent_Identity=26.791277258567, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=35.3846153846154, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=485, Percent_Identity=35.6701030927835, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6323056, Length=487, Percent_Identity=35.1129363449692, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24649832, Length=483, Percent_Identity=37.0600414078675, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_RALSO (Q8Y232)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_518625.1
- ProteinModelPortal:   Q8Y232
- SMR:   Q8Y232
- GeneID:   1219308
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0504
- NMPDR:   fig|267608.1.peg.504
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   RSOL267608:RSC0504-MONOMER
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55847; Mature: 55847

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQQALLSVSDKTGIVDFARALHGAGVKLLSTGGTAKLLAESGLPVTEVADYTGFPEMLD
CCHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCEEEHHHCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALSQHGIPTIDLLVVNLYPFQQTVAKDECTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCHHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQANGNTVGYDTNFMLAKKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHH
AQYDGAITNYLTSLGQDKSHSTRSQYPQTLNLAFEQVQEMRYGENPHQSAAFYRDLKAVD
HHCCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALANYAQLQGKELSYNNIADADAAWECVKSFDPAKGAACVIIKHANPCGVAIGGTAQEA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCHHHH
YEKAFKTDSTSAFGGIIAFNVPLDEAAAQVVAKQFVEVLIAPSFSAGARTVFAAKQNVRL
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEE
LEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQSAELRVVTKRHPTPKEMDDLLFAWRVAK
EEEECCCCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
FVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLMLSGSAVASDAFFPFRDGL
HHHCCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHCCCCCCCCCCCC
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH
EEEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC
>Mature Secondary Structure
MIQQALLSVSDKTGIVDFARALHGAGVKLLSTGGTAKLLAESGLPVTEVADYTGFPEMLD
CCHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCEEEHHHCCCCHHHHHHCCCCHHHHC
GRVKTLHPKVHGGILARRDLPEHMAALSQHGIPTIDLLVVNLYPFQQTVAKDECTLADAI
CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCHHHHHHH
ENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQANGNTVGYDTNFMLAKKVFAHT
HHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHH
AQYDGAITNYLTSLGQDKSHSTRSQYPQTLNLAFEQVQEMRYGENPHQSAAFYRDLKAVD
HHCCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALANYAQLQGKELSYNNIADADAAWECVKSFDPAKGAACVIIKHANPCGVAIGGTAQEA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEECCCHHHH
YEKAFKTDSTSAFGGIIAFNVPLDEAAAQVVAKQFVEVLIAPSFSAGARTVFAAKQNVRL
HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEE
LEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQSAELRVVTKRHPTPKEMDDLLFAWRVAK
EEEECCCCCCCHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
FVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLMLSGSAVASDAFFPFRDGL
HHHCCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHCCCCCCCCCCCC
DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH
EEEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852