Click here to switch to the map view.

The map label for this gene is yieH [H]

Identifier: 17544990

GI number: 17544990

Start: 304154

End: 304843

Strand: Reverse

Name: yieH [H]

Synonym: RSc0271

Alternate gene names: 17544990

Gene position: 304843-304154 (Counterclockwise)

Preceding gene: 17544991

Following gene: 17544989

Centisome position: 8.2

GC content: 71.16

Gene sequence:

>690_bases
ATGACTGAAGTCTCCCACCTCGCCCCCGCCGGCCGCCGCTTCGACGCCATCCTGTTCGATTGCGACGGCGTGCTGGTCGA
CAGCGAACCCCTCGTCAACCGGCTGATCTGGCAGATGCTCAATGAGCTCGGCATCGACATCAGCCTGGAGGACTCCACCC
GGCGCTTCCTCGGCAAGGCCATCCGCGAAGAGCTGGATGCGATTGCCGCGATGCGCGGCGCGCCGCTGCCGGCCGGCTGG
CTGTCCACCTTCCACGCGCGCCGCAATGCGCTGCTGGAAGCCGAAGTGCAGGCCGTGCCCCACGTGACGCAGGCCATCGA
AGCGCTGTCCGCGCTGGGCCTGCCGATGGCGGTCGCCTCCGGCGCCGACCGCCTGAAGGTCGAGCTGCAGCTCAACCGCA
CCGGCCTGATCCACCGCTTCCAACCTACCGATGCGCGCATCTTTTCCGCCACCGAAGTCGAGCGCAGCAAACCCGCGCCC
GACGTCTACCTGCTGGCCGCGCGCCGGCTGGGGGTGGCGCCGTCGCGCTGCGTGGTGATCGAGGACAGCCCCACCGGCGT
CACCGCCGGCCACACCGCCGGCATGACGGTGCTGGCCTACGCCGGCCGCAACGCGCCCGGCCCGCTGATCGCCGCCGGCG
CCGCGCACACCTTCACCGACATGCGCCACCTGCCGGCGCTGCTGGCATGA

Upstream 100 bases:

>100_bases
CCGGCCACACCGGGCGCGTGGCGCATATCGACGGCGGCTTCACCGCCTGGAAGGCGGCCGGCGCGCCGGTCGCCGCTTAC
GAAAAACCCACCAAGAAAGC

Downstream 100 bases:

>100_bases
CGCGCGCCCTGCCCGCCCCGGCCCCGACCGCGGCCTGCCCGTGCGGCGCCGGCGCCTACGGCCGCTGCTGCGGCCCGTTC
CATGCGGGCGACGCGGTGCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTEVSHLAPAGRRFDAILFDCDGVLVDSEPLVNRLIWQMLNELGIDISLEDSTRRFLGKAIREELDAIAAMRGAPLPAGW
LSTFHARRNALLEAEVQAVPHVTQAIEALSALGLPMAVASGADRLKVELQLNRTGLIHRFQPTDARIFSATEVERSKPAP
DVYLLAARRLGVAPSRCVVIEDSPTGVTAGHTAGMTVLAYAGRNAPGPLIAAGAAHTFTDMRHLPALLA

Sequences:

>Translated_229_residues
MTEVSHLAPAGRRFDAILFDCDGVLVDSEPLVNRLIWQMLNELGIDISLEDSTRRFLGKAIREELDAIAAMRGAPLPAGW
LSTFHARRNALLEAEVQAVPHVTQAIEALSALGLPMAVASGADRLKVELQLNRTGLIHRFQPTDARIFSATEVERSKPAP
DVYLLAARRLGVAPSRCVVIEDSPTGVTAGHTAGMTVLAYAGRNAPGPLIAAGAAHTFTDMRHLPALLA
>Mature_228_residues
TEVSHLAPAGRRFDAILFDCDGVLVDSEPLVNRLIWQMLNELGIDISLEDSTRRFLGKAIREELDAIAAMRGAPLPAGWL
STFHARRNALLEAEVQAVPHVTQAIEALSALGLPMAVASGADRLKVELQLNRTGLIHRFQPTDARIFSATEVERSKPAPD
VYLLAARRLGVAPSRCVVIEDSPTGVTAGHTAGMTVLAYAGRNAPGPLIAAGAAHTFTDMRHLPALLA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=216, Percent_Identity=30.0925925925926, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789046, Length=193, Percent_Identity=31.6062176165803, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788021, Length=215, Percent_Identity=32.5581395348837, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082080, Length=196, Percent_Identity=33.1632653061224, Blast_Score=60, Evalue=1e-10,
Organism=Drosophila melanogaster, GI45550911, Length=221, Percent_Identity=30.316742081448, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137324, Length=195, Percent_Identity=31.7948717948718, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116008157, Length=196, Percent_Identity=31.1224489795918, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20129151, Length=187, Percent_Identity=28.8770053475936, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24540; Mature: 24408

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVSHLAPAGRRFDAILFDCDGVLVDSEPLVNRLIWQMLNELGIDISLEDSTRRFLGKA
CCCCCCCCCCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHH
IREELDAIAAMRGAPLPAGWLSTFHARRNALLEAEVQAVPHVTQAIEALSALGLPMAVAS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHC
GADRLKVELQLNRTGLIHRFQPTDARIFSATEVERSKPAPDVYLLAARRLGVAPSRCVVI
CCCEEEEEEEECCCCCEEEECCCCCEEEEHHHHHCCCCCCCEEEEEHHHCCCCCCEEEEE
EDSPTGVTAGHTAGMTVLAYAGRNAPGPLIAAGAAHTFTDMRHLPALLA
ECCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEVSHLAPAGRRFDAILFDCDGVLVDSEPLVNRLIWQMLNELGIDISLEDSTRRFLGKA
CCCCCCCCCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHH
IREELDAIAAMRGAPLPAGWLSTFHARRNALLEAEVQAVPHVTQAIEALSALGLPMAVAS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHC
GADRLKVELQLNRTGLIHRFQPTDARIFSATEVERSKPAPDVYLLAARRLGVAPSRCVVI
CCCEEEEEEEECCCCCEEEECCCCCEEEEHHHHHCCCCCCCEEEEEHHHCCCCCCEEEEE
EDSPTGVTAGHTAGMTVLAYAGRNAPGPLIAAGAAHTFTDMRHLPALLA
ECCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]