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The map label for this gene is dapF [H]

Identifier: 17544856

GI number: 17544856

Start: 159639

End: 160517

Strand: Direct

Name: dapF [H]

Synonym: RSc0137

Alternate gene names: 17544856

Gene position: 159639-160517 (Clockwise)

Preceding gene: 17544855

Following gene: 17544857

Centisome position: 4.3

GC content: 69.74

Gene sequence:

>879_bases
ATGAAACTGCACTTCACCAAGATGCACGGCGCCGGCAACGACTTCGTCGTGCTCGACGGCATCGCGACCCCGATCGATTT
CACCCCCGAACAGTGGCGCGCCATCGCCGACCGCCACTTCGGCGTGGGCGCGGACCAGCTGCTGCTGGTGGAGCGTTCCA
CCCGCCCCGACGTCGATTTCCGCTACCGCATCTTCAACCACGACGGCGGCGAGGTGGAGCAGTGCGGCAACGGCGCGCGC
TGCTTCGTCAAGTTCGTTACCGACCGTGGCCTGACCGACAAGCGCACCATCCGCGTCGAAGTGATGAACGGCATCTCCAC
GCTGACGATGCAGCCCGACGGCCAGGTGACGGTCGACATGGGCGCCCCGGTGTTCGAGGCCGCGCGCCTGCCCTTCGTGC
CCGATGCCCTGCCCACCCGCGTGGAAGGCCGCGACACGCAGCACGCCCTGCAGATCAACGGCCGCACCGCGTGGCTGTCC
ACCGTGTCGATGGGCAACCCGCACGCGGTGCAGGTGGTCGATGACGCGGAAGCCTTCCCGGTGCGCGAAGACGGCCCGTT
GATCGAATCGCACGCTGTGTTCCCGAGGCGCGTGAATGCCGGCTTCATGGAGATCGCCGACCGCCACGCCATCCGCCTGC
GCGTGTACGAGCGCGGCGCCGGCGAGACGCTGGCCTGTGGCACGGGCGCCTGCGCGGCGGCGGTGGCGGGGATCCGGCGC
GGCCTGCTGGATTCGCCCGTCAAGGTGACCACGCACGGCGGCGACCTGACCATCGCCTGGGCCGGCGAGGGCGAGCCGGT
GATGATGACCGGCCCGGCCACGACCGTGTTCGAAGGCACGCTGGACCTGGACGCACTGAAGCAGACCGCCGCGCACTGA

Upstream 100 bases:

>100_bases
CGGCGAGCCGGGCATCTACTGAGCGGGCCGCTGCGTTCGGCCGGCTCGTTTTAGAATGGGCGCTTTCCGAACGCTGACCG
CCGCCCCCGCGCGGCCTGCC

Downstream 100 bases:

>100_bases
GCGCGGCAGGCCGCGGCGCCGGCCGCTGCGTCAGAACGACAACAAGGAGGCTCGATGGGATCGAACGGCAAGACCCCGTT
GGACGATTGGCGATTCGACG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKLHFTKMHGAGNDFVVLDGIATPIDFTPEQWRAIADRHFGVGADQLLLVERSTRPDVDFRYRIFNHDGGEVEQCGNGAR
CFVKFVTDRGLTDKRTIRVEVMNGISTLTMQPDGQVTVDMGAPVFEAARLPFVPDALPTRVEGRDTQHALQINGRTAWLS
TVSMGNPHAVQVVDDAEAFPVREDGPLIESHAVFPRRVNAGFMEIADRHAIRLRVYERGAGETLACGTGACAAAVAGIRR
GLLDSPVKVTTHGGDLTIAWAGEGEPVMMTGPATTVFEGTLDLDALKQTAAH

Sequences:

>Translated_292_residues
MKLHFTKMHGAGNDFVVLDGIATPIDFTPEQWRAIADRHFGVGADQLLLVERSTRPDVDFRYRIFNHDGGEVEQCGNGAR
CFVKFVTDRGLTDKRTIRVEVMNGISTLTMQPDGQVTVDMGAPVFEAARLPFVPDALPTRVEGRDTQHALQINGRTAWLS
TVSMGNPHAVQVVDDAEAFPVREDGPLIESHAVFPRRVNAGFMEIADRHAIRLRVYERGAGETLACGTGACAAAVAGIRR
GLLDSPVKVTTHGGDLTIAWAGEGEPVMMTGPATTVFEGTLDLDALKQTAAH
>Mature_292_residues
MKLHFTKMHGAGNDFVVLDGIATPIDFTPEQWRAIADRHFGVGADQLLLVERSTRPDVDFRYRIFNHDGGEVEQCGNGAR
CFVKFVTDRGLTDKRTIRVEVMNGISTLTMQPDGQVTVDMGAPVFEAARLPFVPDALPTRVEGRDTQHALQINGRTAWLS
TVSMGNPHAVQVVDDAEAFPVREDGPLIESHAVFPRRVNAGFMEIADRHAIRLRVYERGAGETLACGTGACAAAVAGIRR
GLLDSPVKVTTHGGDLTIAWAGEGEPVMMTGPATTVFEGTLDLDALKQTAAH

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=51.9572953736655, Blast_Score=290, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 31628; Mature: 31628

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHFTKMHGAGNDFVVLDGIATPIDFTPEQWRAIADRHFGVGADQLLLVERSTRPDVDF
CEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEE
RYRIFNHDGGEVEQCGNGARCFVKFVTDRGLTDKRTIRVEVMNGISTLTMQPDGQVTVDM
EEEEEECCCCCHHHCCCCCEEEEEEEECCCCCCCEEEEEEEECCCEEEEECCCCCEEEEC
GAPVFEAARLPFVPDALPTRVEGRDTQHALQINGRTAWLSTVSMGNPHAVQVVDDAEAFP
CCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEEEECCCCCC
VREDGPLIESHAVFPRRVNAGFMEIADRHAIRLRVYERGAGETLACGTGACAAAVAGIRR
CCCCCCEEECCCCCCEECCCCHHHHHCCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHH
GLLDSPVKVTTHGGDLTIAWAGEGEPVMMTGPATTVFEGTLDLDALKQTAAH
HHCCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MKLHFTKMHGAGNDFVVLDGIATPIDFTPEQWRAIADRHFGVGADQLLLVERSTRPDVDF
CEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEE
RYRIFNHDGGEVEQCGNGARCFVKFVTDRGLTDKRTIRVEVMNGISTLTMQPDGQVTVDM
EEEEEECCCCCHHHCCCCCEEEEEEEECCCCCCCEEEEEEEECCCEEEEECCCCCEEEEC
GAPVFEAARLPFVPDALPTRVEGRDTQHALQINGRTAWLSTVSMGNPHAVQVVDDAEAFP
CCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCEEEEEEECCCCCC
VREDGPLIESHAVFPRRVNAGFMEIADRHAIRLRVYERGAGETLACGTGACAAAVAGIRR
CCCCCCEEECCCCCCEECCCCHHHHHCCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHH
GLLDSPVKVTTHGGDLTIAWAGEGEPVMMTGPATTVFEGTLDLDALKQTAAH
HHCCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA