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The map label for this gene is htrB [H]

Identifier: 17544855

GI number: 17544855

Start: 158667

End: 159560

Strand: Direct

Name: htrB [H]

Synonym: RSc0136

Alternate gene names: 17544855

Gene position: 158667-159560 (Clockwise)

Preceding gene: 17544854

Following gene: 17544856

Centisome position: 4.27

GC content: 67.23

Gene sequence:

>894_bases
ATGGAACGTTTCACTGCCAAACTCGGACTCGGCTTCCTGTGGCTGCTGTCCTTCTTCCCGTACGGTTTCGTCGCCCGCTT
CGGCGAAGGGCTGGGCGGGCTGCTGTATCGCATCCCCAACGGCCGCCGCCGCGTGGTGCTGGCCAACCTGAAGGCGTGCT
TCCCCGAAAAGTCCGACGCCGAGCGCGAGGCCATCGCGCACGAGGTCTTCCGCAAGGTGTTCCGCAGCTTCGCCGAGCGC
TCGTTCGCGTGGTTCGCGTCCGAGCAGCGGCTCAAGCGCGTGGTGAAGATCGACGACCAAGCCAACCTGCCCGCACTGCA
CGGCTCGCCGCACATTCTCGTCACGCTGCACCTGTCGGGCGTGGAGATCGGCGCGCTGGCGCTGACGGACTACCTGCGCG
AGCACGTCGGCAACGCCGGCTGCTCGCTCTACACGCGCATGGCCAACCCGGCGCTGGATGCGGCGATCAAGGCGCTGCGC
AGCCGCTTCGGCGCGACCATGGTGTCGCGCAACGAGAACATCCGCCAGATCATGCGCACCATCAAGCGCGGCGAGGCGCT
GCAGCTGATCTCCGACATGGACTTCGGCGAGCGCGACGCCGAGTTCGTGCCGTTTTTCGGCGTGCAAGCGCTCACGCTCA
ACGCCATCCCGCGCATGGCCGCGCTGACCGGCGCCAGGGTGGTCCCGATGTACACCGAGATCCTGCCGGACTACCAGGGC
TACGTGCTGCGCATCCTGCCGGCCTGGGACGACTATCCGACCGGCGACCTGAGCGCCGATACGCGCCGCATGAACGCCTT
CTTCGAAGACGCCATCCGCCCGCGCATCACCGAGTACTACTGGGTGCACAAGCGCTTCAAGCATCGCCCGCCCGGCGAGC
CGGGCATCTACTGA

Upstream 100 bases:

>100_bases
TTGCCCTGGCACCGACGCAATATCTCTGGGGCTATAACCGCTACAAGCGACCGAAGGGCGTGGACGCCCCGCCCGCTGCC
TGACGCTGCTGCCGCACTTT

Downstream 100 bases:

>100_bases
GCGGGCCGCTGCGTTCGGCCGGCTCGTTTTAGAATGGGCGCTTTCCGAACGCTGACCGCCGCCCCCGCGCGGCCTGCCAT
GAAACTGCACTTCACCAAGA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MERFTAKLGLGFLWLLSFFPYGFVARFGEGLGGLLYRIPNGRRRVVLANLKACFPEKSDAEREAIAHEVFRKVFRSFAER
SFAWFASEQRLKRVVKIDDQANLPALHGSPHILVTLHLSGVEIGALALTDYLREHVGNAGCSLYTRMANPALDAAIKALR
SRFGATMVSRNENIRQIMRTIKRGEALQLISDMDFGERDAEFVPFFGVQALTLNAIPRMAALTGARVVPMYTEILPDYQG
YVLRILPAWDDYPTGDLSADTRRMNAFFEDAIRPRITEYYWVHKRFKHRPPGEPGIY

Sequences:

>Translated_297_residues
MERFTAKLGLGFLWLLSFFPYGFVARFGEGLGGLLYRIPNGRRRVVLANLKACFPEKSDAEREAIAHEVFRKVFRSFAER
SFAWFASEQRLKRVVKIDDQANLPALHGSPHILVTLHLSGVEIGALALTDYLREHVGNAGCSLYTRMANPALDAAIKALR
SRFGATMVSRNENIRQIMRTIKRGEALQLISDMDFGERDAEFVPFFGVQALTLNAIPRMAALTGARVVPMYTEILPDYQG
YVLRILPAWDDYPTGDLSADTRRMNAFFEDAIRPRITEYYWVHKRFKHRPPGEPGIY
>Mature_297_residues
MERFTAKLGLGFLWLLSFFPYGFVARFGEGLGGLLYRIPNGRRRVVLANLKACFPEKSDAEREAIAHEVFRKVFRSFAER
SFAWFASEQRLKRVVKIDDQANLPALHGSPHILVTLHLSGVEIGALALTDYLREHVGNAGCSLYTRMANPALDAAIKALR
SRFGATMVSRNENIRQIMRTIKRGEALQLISDMDFGERDAEFVPFFGVQALTLNAIPRMAALTGARVVPMYTEILPDYQG
YVLRILPAWDDYPTGDLSADTRRMNAFFEDAIRPRITEYYWVHKRFKHRPPGEPGIY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=293, Percent_Identity=31.740614334471, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI87082094, Length=293, Percent_Identity=29.6928327645051, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788161, Length=302, Percent_Identity=29.1390728476821, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 33761; Mature: 33761

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFTAKLGLGFLWLLSFFPYGFVARFGEGLGGLLYRIPNGRRRVVLANLKACFPEKSDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEEEEHHHHCCCCCCH
EREAIAHEVFRKVFRSFAERSFAWFASEQRLKRVVKIDDQANLPALHGSPHILVTLHLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
VEIGALALTDYLREHVGNAGCSLYTRMANPALDAAIKALRSRFGATMVSRNENIRQIMRT
CCHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHH
IKRGEALQLISDMDFGERDAEFVPFFGVQALTLNAIPRMAALTGARVVPMYTEILPDYQG
HHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
YVLRILPAWDDYPTGDLSADTRRMNAFFEDAIRPRITEYYWVHKRFKHRPPGEPGIY
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MERFTAKLGLGFLWLLSFFPYGFVARFGEGLGGLLYRIPNGRRRVVLANLKACFPEKSDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCEEEEEEHHHHCCCCCCH
EREAIAHEVFRKVFRSFAERSFAWFASEQRLKRVVKIDDQANLPALHGSPHILVTLHLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
VEIGALALTDYLREHVGNAGCSLYTRMANPALDAAIKALRSRFGATMVSRNENIRQIMRT
CCHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHH
IKRGEALQLISDMDFGERDAEFVPFFGVQALTLNAIPRMAALTGARVVPMYTEILPDYQG
HHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
YVLRILPAWDDYPTGDLSADTRRMNAFFEDAIRPRITEYYWVHKRFKHRPPGEPGIY
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]