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The map label for this gene is gshB

Identifier: 17231351

GI number: 17231351

Start: 4657465

End: 4658439

Strand: Reverse

Name: gshB

Synonym: all3859

Alternate gene names: 17231351

Gene position: 4658439-4657465 (Counterclockwise)

Preceding gene: 17231352

Following gene: 17231348

Centisome position: 72.63

GC content: 44.0

Gene sequence:

>975_bases
GTGAAACTGGCTTTTATTATAGATCCCATCCATCAGTTAGACCCATGCCATGATACCAGTGTGGCGCTGATGGAAGCAGC
GCAAATTTTAGGACATGAAGTTTGGGTAACTCAGGCTAATTGGTTGAGTGTAGTAGACAGCAAAGCTTGGGCTATATTGC
AGCAGGTAGAACTTGTACCAGTGCAGCTAATAGATGGACGATGGGTGGCAGCAAGTCCTTGGTATACATTAAATACTCGT
TCCTTCAGTTCTTTGGAGACGATGGATGCTGTATTCATGCGGACAGATCCGCCCGTGAACGATGCCTATCTTTACGCTAC
CTATGTTCTAGATTACGTTGATCAACGTAAAACTTTGGTAATTAACAATCCCAATGGTATCCGTGGGGCAAATGAAAAAA
TGTATGCCCTTCAATTTACCAAGGCAATTCCCGAAACCATCGTCAGTGCAGATAAAGATTTTATCCGGCAATTTGTCGAA
GCAAAAGGCGCTACTGTTCTCAAACCCTTGGGTAACAAAGCTGGGGAAGGGATTCTATTTCTACAAGCAGGCGATCGCAA
TTTTAACTCCATCGTCGAACTCAGCACCCAGCAAGGCCGATTACCCGTAATGGTACAAACTTATCTACCAGAGGCCAAAG
AGGGAGATAAGCGGATCATTTTACTTAATGGTGAACCCATTGGCGCTCTCAATCGTTTGGCAAGTGGCAGTGATTTTCGT
AATAACATGGCCACTGGTGGGACGGTTGCAAAAACCGAAATTACCCCACGAGAAGAAGAAATTTGTAGCCAAATCGCCGC
AAATTTACGCCAAGATGGGTTAATCTTTGTGGGTATTGATGTGATAGGTGGCTATCTCACAGAAGTTAACGTCACTAGCC
CAACTGGGATAAGGGAAATCGACCGACTAGATGGTACTCGCCTGGCTCATCAAGTAATTCAATGGGTTGAAAAAAACCTA
CAAATTCAAAATTAA

Upstream 100 bases:

>100_bases
AGCAACAGGATTACGCTTTTTTCCTCACCACTAATTGTCAATCTGTCAGGATATCGAGGGATAATTTATCTGAGAATTTC
CTGGCGTTGAGGCAAAAGGC

Downstream 100 bases:

>100_bases
ACACAGGTAATCAAAGGAAAACTACAGGAGGAGGACGTGAAGATTTCTCTTTTCGGTCTCACTCCTGTGTATCACAAATA
CACAATTAGGCGTAAAAGTC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVPVQLIDGRWVAASPWYTLNTR
SFSSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQRKTLVINNPNGIRGANEKMYALQFTKAIPETIVSADKDFIRQFVE
AKGATVLKPLGNKAGEGILFLQAGDRNFNSIVELSTQQGRLPVMVQTYLPEAKEGDKRIILLNGEPIGALNRLASGSDFR
NNMATGGTVAKTEITPREEEICSQIAANLRQDGLIFVGIDVIGGYLTEVNVTSPTGIREIDRLDGTRLAHQVIQWVEKNL
QIQN

Sequences:

>Translated_324_residues
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVPVQLIDGRWVAASPWYTLNTR
SFSSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQRKTLVINNPNGIRGANEKMYALQFTKAIPETIVSADKDFIRQFVE
AKGATVLKPLGNKAGEGILFLQAGDRNFNSIVELSTQQGRLPVMVQTYLPEAKEGDKRIILLNGEPIGALNRLASGSDFR
NNMATGGTVAKTEITPREEEICSQIAANLRQDGLIFVGIDVIGGYLTEVNVTSPTGIREIDRLDGTRLAHQVIQWVEKNL
QIQN
>Mature_324_residues
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVPVQLIDGRWVAASPWYTLNTR
SFSSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQRKTLVINNPNGIRGANEKMYALQFTKAIPETIVSADKDFIRQFVE
AKGATVLKPLGNKAGEGILFLQAGDRNFNSIVELSTQQGRLPVMVQTYLPEAKEGDKRIILLNGEPIGALNRLASGSDFR
NNMATGGTVAKTEITPREEEICSQIAANLRQDGLIFVGIDVIGGYLTEVNVTSPTGIREIDRLDGTRLAHQVIQWVEKNL
QIQN

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=324, Percent_Identity=33.641975308642, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787076, Length=248, Percent_Identity=25.8064516129032, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_NOSS1 (P45480)

Other databases:

- EMBL:   U14408
- EMBL:   BA000019
- EMBL:   Z31371
- PIR:   AD2288
- PIR:   JC4291
- RefSeq:   NP_487899.1
- ProteinModelPortal:   P45480
- SMR:   P45480
- STRING:   P45480
- GeneID:   1107457
- GenomeReviews:   BA000019_GR
- KEGG:   ana:all3859
- NMPDR:   fig|103690.1.peg.4166
- eggNOG:   COG0189
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- PhylomeDB:   P45480
- ProtClustDB:   PRK05246
- BioCyc:   NSP103690:ALL3859-MONOMER
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 35954; Mature: 35954

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVP
CEEEEEECCHHHCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHEEE
VQLIDGRWVAASPWYTLNTRSFSSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQRKTLV
EEEECCEEEEECCCEEECCCCCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCEEE
INNPNGIRGANEKMYALQFTKAIPETIVSADKDFIRQFVEAKGATVLKPLGNKAGEGILF
EECCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCEEEE
LQAGDRNFNSIVELSTQQGRLPVMVQTYLPEAKEGDKRIILLNGEPIGALNRLASGSDFR
EEECCCCCCHHEEECCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHCCCCCHH
NNMATGGTVAKTEITPREEEICSQIAANLRQDGLIFVGIDVIGGYLTEVNVTSPTGIREI
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCEEEEEECCCCCCHHHH
DRLDGTRLAHQVIQWVEKNLQIQN
HHCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVP
CEEEEEECCHHHCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHEEE
VQLIDGRWVAASPWYTLNTRSFSSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQRKTLV
EEEECCEEEEECCCEEECCCCCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCEEE
INNPNGIRGANEKMYALQFTKAIPETIVSADKDFIRQFVEAKGATVLKPLGNKAGEGILF
EECCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCEEEE
LQAGDRNFNSIVELSTQQGRLPVMVQTYLPEAKEGDKRIILLNGEPIGALNRLASGSDFR
EEECCCCCCHHEEECCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHCCCCCHH
NNMATGGTVAKTEITPREEEICSQIAANLRQDGLIFVGIDVIGGYLTEVNVTSPTGIREI
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCEEEEEECCCCCCHHHH
DRLDGTRLAHQVIQWVEKNLQIQN
HHCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7557485; 11759840; 8525061