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The map label for this gene is hsdBR [H]

Identifier: 17231193

GI number: 17231193

Start: 4469152

End: 4470792

Strand: Direct

Name: hsdBR [H]

Synonym: alr3701

Alternate gene names: 17231193

Gene position: 4469152-4470792 (Clockwise)

Preceding gene: 17231192

Following gene: 17231195

Centisome position: 69.68

GC content: 41.26

Gene sequence:

>1641_bases
GTGAATGAGAATGATTCAGACAGTTATATTCATATTATTACAAACTCTCTAGAAGATTCATTGCGAGTGCAAATGGATCA
ATTCTCATCTACCTTAGATGAACTTGGGTTAGCAGTGTCAACGGGGCCAGTTGTAGATTTTCGCCTCAAATCAGCTTTGA
GAAATTATGTGAATGAGGAAACTGTTCCATTACTTTACCCAGAAGCAATAAAAACAGGGAAAGTTTTATTTCCGCCAAAA
AAGCCGCGTAAGTCCATCGCCATTGTACAAAATCAAGAAACAGACAAATGGCTAATTCCATCAGGTTGGTATGTTTTGAC
GAAGCGATTTTCTGCTAAAGAAGAAAAACGGCGGGTTGTTGCTGCTGTATGTTCTCCTGTAGATGCACCAGTGTTGGGCA
TAGAAAATCACCTCAACTACTACCATTCTCAAGGTGAGGGAATGAATCCCGACCTGGCACGAGGTTTAGCAGCATTTCTC
AGTTCAACTTTATTGGATAGTTACTTCCGCCTATTCAGTGGACATACACAAGTAAATGCAACGGATTTGCGTAGAATTAA
GTATCCTTGCAAAGATGATTTAATCAAGCTAGGTAGTCAAATTGGTGATTCTTGCCTTGATCAGGCTCAACTTGATACGG
TTGTACATAAAACTCTATCGATTATGAGCGAGGCAATAAAGGCAGTTCTGGCTGCTAAACGAATTGAGGAGGCGTTGGCA
ATTCTTAAAGATATTTCTGCTCCCAAAGAGCAACAGAACGAGCGTTCAGCACTTTTCTTACTTGCATTGGCAGATATTCG
TCCGGAAATTCCTTGGACTCAAGCTACATCACCAAGACGCAGAATTACAGAAATGATGGATTGGTTTCGTGATCACTATG
GTAAACAATACGCACCGAATACACGCGAAACAGTCAGACGGCAGACGATGCACCAGTTTGTGCAGATGGGGATAGTTGTT
GAGAATCCAGATCAACCAGATCGACCGATTAACAGTCCCAAATGGTGTTATCAACTTCATCAACAATTTGTGACACTGCT
TAAATCATACGGTTCCGAGCAGTGGGAAGAGACTCGTCGTAATTATGTTATTTCAGTTAAAAACTTATTGCAGGACAGAA
ATCGAAATATCCCGATGATTCCCGTAAGTTTGCCTAACGGTCAAGCAATTCAACTATCATCAGGTGGACAAAATATATTG
ATTAAAGAAATTCTGGAAAACTTTTGTCCCAGATTTACACCTGAAGGTTTAGTTCTGTTTGTAGGTGATGCTGGAAATAA
GTTTATTGTTAATGAGACTCAGAAGTTTCGAGAAATTGGGATCGAGTTAGATCCTCACGGTAAGATGCCAGACATTGTGG
TTTACTACGAACGGCAAGAGTGGCTTGTCTTGATAGAAGCGGTGACTAGTCATGGTCCGGTCAATCTAAAACGTCGCAAC
GAATTAAAGCGGCTCTTTCAATCTAGCCGTCAGGGGTTAGTTTTCGTTACTGCTTTCCCCAGTCGCAAGGAAATGACTCG
CTATCTTGCTGAAATCTCTTGGGAGACAGAAGTTTGGGTAGCAGCTCAACCTGATCACATGATTCATTTCAATGGAGAAA
GATTTCTGGGACCCTATGAAGACCGAGAAAATAGGTTTTGA

Upstream 100 bases:

>100_bases
TAAAACTACAGAAGATCGTAGTTACGTAGAGATTACTAATAATTTGGAAGCCCAATCAGATGGGATTTTAGAATTAAGAA
ATATTCCCTACAACGAAGTT

Downstream 100 bases:

>100_bases
GCAATAATGTTAGAAGCCCCTAGATTGATTCTGTTCTCGGACTTTTACCCTATATTATTGTGGTGTTTTAACAGGTTTTA
AATTTCTCGCTTTATAAAAC

Product: type II site-specific deoxyribonuclease

Products: NA

Alternate protein names: R.BsuBI; Endonuclease BsuBI; Type II restriction enzyme BsuBI [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MNENDSDSYIHIITNSLEDSLRVQMDQFSSTLDELGLAVSTGPVVDFRLKSALRNYVNEETVPLLYPEAIKTGKVLFPPK
KPRKSIAIVQNQETDKWLIPSGWYVLTKRFSAKEEKRRVVAAVCSPVDAPVLGIENHLNYYHSQGEGMNPDLARGLAAFL
SSTLLDSYFRLFSGHTQVNATDLRRIKYPCKDDLIKLGSQIGDSCLDQAQLDTVVHKTLSIMSEAIKAVLAAKRIEEALA
ILKDISAPKEQQNERSALFLLALADIRPEIPWTQATSPRRRITEMMDWFRDHYGKQYAPNTRETVRRQTMHQFVQMGIVV
ENPDQPDRPINSPKWCYQLHQQFVTLLKSYGSEQWEETRRNYVISVKNLLQDRNRNIPMIPVSLPNGQAIQLSSGGQNIL
IKEILENFCPRFTPEGLVLFVGDAGNKFIVNETQKFREIGIELDPHGKMPDIVVYYERQEWLVLIEAVTSHGPVNLKRRN
ELKRLFQSSRQGLVFVTAFPSRKEMTRYLAEISWETEVWVAAQPDHMIHFNGERFLGPYEDRENRF

Sequences:

>Translated_546_residues
MNENDSDSYIHIITNSLEDSLRVQMDQFSSTLDELGLAVSTGPVVDFRLKSALRNYVNEETVPLLYPEAIKTGKVLFPPK
KPRKSIAIVQNQETDKWLIPSGWYVLTKRFSAKEEKRRVVAAVCSPVDAPVLGIENHLNYYHSQGEGMNPDLARGLAAFL
SSTLLDSYFRLFSGHTQVNATDLRRIKYPCKDDLIKLGSQIGDSCLDQAQLDTVVHKTLSIMSEAIKAVLAAKRIEEALA
ILKDISAPKEQQNERSALFLLALADIRPEIPWTQATSPRRRITEMMDWFRDHYGKQYAPNTRETVRRQTMHQFVQMGIVV
ENPDQPDRPINSPKWCYQLHQQFVTLLKSYGSEQWEETRRNYVISVKNLLQDRNRNIPMIPVSLPNGQAIQLSSGGQNIL
IKEILENFCPRFTPEGLVLFVGDAGNKFIVNETQKFREIGIELDPHGKMPDIVVYYERQEWLVLIEAVTSHGPVNLKRRN
ELKRLFQSSRQGLVFVTAFPSRKEMTRYLAEISWETEVWVAAQPDHMIHFNGERFLGPYEDRENRF
>Mature_546_residues
MNENDSDSYIHIITNSLEDSLRVQMDQFSSTLDELGLAVSTGPVVDFRLKSALRNYVNEETVPLLYPEAIKTGKVLFPPK
KPRKSIAIVQNQETDKWLIPSGWYVLTKRFSAKEEKRRVVAAVCSPVDAPVLGIENHLNYYHSQGEGMNPDLARGLAAFL
SSTLLDSYFRLFSGHTQVNATDLRRIKYPCKDDLIKLGSQIGDSCLDQAQLDTVVHKTLSIMSEAIKAVLAAKRIEEALA
ILKDISAPKEQQNERSALFLLALADIRPEIPWTQATSPRRRITEMMDWFRDHYGKQYAPNTRETVRRQTMHQFVQMGIVV
ENPDQPDRPINSPKWCYQLHQQFVTLLKSYGSEQWEETRRNYVISVKNLLQDRNRNIPMIPVSLPNGQAIQLSSGGQNIL
IKEILENFCPRFTPEGLVLFVGDAGNKFIVNETQKFREIGIELDPHGKMPDIVVYYERQEWLVLIEAVTSHGPVNLKRRN
ELKRLFQSSRQGLVFVTAFPSRKEMTRYLAEISWETEVWVAAQPDHMIHFNGERFLGPYEDRENRF

Specific function: Recognizes the double-stranded sequence CTGCAG and cleaves after A-5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009528 [H]

Pfam domain/function: PF06616 BsuBI_PstI_RE [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 62663; Mature: 62663

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENDSDSYIHIITNSLEDSLRVQMDQFSSTLDELGLAVSTGPVVDFRLKSALRNYVNEE
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCCC
TVPLLYPEAIKTGKVLFPPKKPRKSIAIVQNQETDKWLIPSGWYVLTKRFSAKEEKRRVV
CCCEEEHHHHCCCCEECCCCCCCCCEEEEECCCCCCEECCCCCEEEHHHHCCHHHHHHHH
AAVCSPVDAPVLGIENHLNYYHSQGEGMNPDLARGLAAFLSSTLLDSYFRLFSGHTQVNA
HHHHCCCCCCEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TDLRRIKYPCKDDLIKLGSQIGDSCLDQAQLDTVVHKTLSIMSEAIKAVLAAKRIEEALA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKDISAPKEQQNERSALFLLALADIRPEIPWTQATSPRRRITEMMDWFRDHYGKQYAPN
HHHHCCCCHHHCCCHHHEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
TRETVRRQTMHQFVQMGIVVENPDQPDRPINSPKWCYQLHQQFVTLLKSYGSEQWEETRR
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
NYVISVKNLLQDRNRNIPMIPVSLPNGQAIQLSSGGQNILIKEILENFCPRFTPEGLVLF
CEEEEHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEE
VGDAGNKFIVNETQKFREIGIELDPHGKMPDIVVYYERQEWLVLIEAVTSHGPVNLKRRN
EECCCCEEEECCHHHHHHCCCEECCCCCCCCEEEEEECCCCEEEEEHHHCCCCCCCHHHH
ELKRLFQSSRQGLVFVTAFPSRKEMTRYLAEISWETEVWVAAQPDHMIHFNGERFLGPYE
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCC
DRENRF
CCCCCC
>Mature Secondary Structure
MNENDSDSYIHIITNSLEDSLRVQMDQFSSTLDELGLAVSTGPVVDFRLKSALRNYVNEE
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHCCCC
TVPLLYPEAIKTGKVLFPPKKPRKSIAIVQNQETDKWLIPSGWYVLTKRFSAKEEKRRVV
CCCEEEHHHHCCCCEECCCCCCCCCEEEEECCCCCCEECCCCCEEEHHHHCCHHHHHHHH
AAVCSPVDAPVLGIENHLNYYHSQGEGMNPDLARGLAAFLSSTLLDSYFRLFSGHTQVNA
HHHHCCCCCCEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TDLRRIKYPCKDDLIKLGSQIGDSCLDQAQLDTVVHKTLSIMSEAIKAVLAAKRIEEALA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKDISAPKEQQNERSALFLLALADIRPEIPWTQATSPRRRITEMMDWFRDHYGKQYAPN
HHHHCCCCHHHCCCHHHEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
TRETVRRQTMHQFVQMGIVVENPDQPDRPINSPKWCYQLHQQFVTLLKSYGSEQWEETRR
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
NYVISVKNLLQDRNRNIPMIPVSLPNGQAIQLSSGGQNILIKEILENFCPRFTPEGLVLF
CEEEEHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEE
VGDAGNKFIVNETQKFREIGIELDPHGKMPDIVVYYERQEWLVLIEAVTSHGPVNLKRRN
EECCCCEEEECCHHHHHHCCCEECCCCCCCCEEEEEECCCCEEEEEHHHCCCCCCCHHHH
ELKRLFQSSRQGLVFVTAFPSRKEMTRYLAEISWETEVWVAAQPDHMIHFNGERFLGPYE
HHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCC
DRENRF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1480472 [H]