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The map label for this gene is pstIM [H]

Identifier: 17231192

GI number: 17231192

Start: 4468308

End: 4469018

Strand: Direct

Name: pstIM [H]

Synonym: alr3700

Alternate gene names: 17231192

Gene position: 4468308-4469018 (Clockwise)

Preceding gene: 17231191

Following gene: 17231193

Centisome position: 69.67

GC content: 35.16

Gene sequence:

>711_bases
ATGACAACCTTCCTGACAGACTCCACTGACATCAATAGAATTAATTTCTCCTCAAGACTGAATTTGCATCGTCGCAGAGA
ACTAGGACAGTTTTTAACTCCTGCACCACTAGCCCGTTTTATGGTGGGACAATTTAATAGTCTATTGGGTCATATTAGTC
TTATAGATCCTGGTGCTGGGGTTGGATCATTAACTGCTGCCTTTGTGGAGCGACTGTTGGCAAATTCTCATGAGGTTAAG
AGTTGCTTTATTACAGTATATGAAGTTGAACCCATATTTATATTACCTCTAAAGCAATGCCTTATAGATTGTTGCACCGC
TTTAGAAAATAAAGGAATTGCCGCTAATTATTGTTTGTATGAAAAAAACTTCATTGATGCTAATAGTGAAATAAATTTAC
CCCTATTATCAACATCTGCCATCAATTTTACCCATGCAATTATCAATCCGCTATACAAGAAGATAAATAACAAATCAATT
GAAAAGAAAATAATTTCTCAAATGGGAATTGAGACTGTAAATCTATATAGTGCATTTGTTTGGCTGACTATTTTACGACT
AGTTGAGGATGGAGAGATAGTTGCAATTACTCCCAGAAGTTTCTGTAATGGGAGTTATTTTAGACATTTCCGTAAATCTT
TTTTAGAACAGATGAAGCTGGAAAAAAATCCAAATTTTCACAAGTCGTTCAGAAGCTTTTCCGGAATATGA

Upstream 100 bases:

>100_bases
TAGAATGATAAGTCCTAATTGCGAGCATATTTTGTACTACTGTGTATATACTAGGTCGCAAGCCGGACAACTATGGCATC
TTTGAGACTCAATTGATTGC

Downstream 100 bases:

>100_bases
AATTTTACAAGAGAATATTATTTTTCATGCTATTAAAACTACAGAAGATCGTAGTTACGTAGAGATTACTAATAATTTGG
AAGCCCAATCAGATGGGATT

Product: site-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.PstI; Adenine-specific methyltransferase PstI [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTTFLTDSTDINRINFSSRLNLHRRRELGQFLTPAPLARFMVGQFNSLLGHISLIDPGAGVGSLTAAFVERLLANSHEVK
SCFITVYEVEPIFILPLKQCLIDCCTALENKGIAANYCLYEKNFIDANSEINLPLLSTSAINFTHAIINPLYKKINNKSI
EKKIISQMGIETVNLYSAFVWLTILRLVEDGEIVAITPRSFCNGSYFRHFRKSFLEQMKLEKNPNFHKSFRSFSGI

Sequences:

>Translated_236_residues
MTTFLTDSTDINRINFSSRLNLHRRRELGQFLTPAPLARFMVGQFNSLLGHISLIDPGAGVGSLTAAFVERLLANSHEVK
SCFITVYEVEPIFILPLKQCLIDCCTALENKGIAANYCLYEKNFIDANSEINLPLLSTSAINFTHAIINPLYKKINNKSI
EKKIISQMGIETVNLYSAFVWLTILRLVEDGEIVAITPRSFCNGSYFRHFRKSFLEQMKLEKNPNFHKSFRSFSGI
>Mature_235_residues
TTFLTDSTDINRINFSSRLNLHRRRELGQFLTPAPLARFMVGQFNSLLGHISLIDPGAGVGSLTAAFVERLLANSHEVKS
CFITVYEVEPIFILPLKQCLIDCCTALENKGIAANYCLYEKNFIDANSEINLPLLSTSAINFTHAIINPLYKKINNKSIE
KKIISQMGIETVNLYSAFVWLTILRLVEDGEIVAITPRSFCNGSYFRHFRKSFLEQMKLEKNPNFHKSFRSFSGI

Specific function: This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002296
- InterPro:   IPR011639 [H]

Pfam domain/function: PF07669 Eco57I [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 26734; Mature: 26603

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFLTDSTDINRINFSSRLNLHRRRELGQFLTPAPLARFMVGQFNSLLGHISLIDPGAG
CCCEECCCCCCCEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECCCCC
VGSLTAAFVERLLANSHEVKSCFITVYEVEPIFILPLKQCLIDCCTALENKGIAANYCLY
HHHHHHHHHHHHHCCCCCHHEEEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEE
EKNFIDANSEINLPLLSTSAINFTHAIINPLYKKINNKSIEKKIISQMGIETVNLYSAFV
ECCEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
WLTILRLVEDGEIVAITPRSFCNGSYFRHFRKSFLEQMKLEKNPNFHKSFRSFSGI
HHHHHHHHCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TTFLTDSTDINRINFSSRLNLHRRRELGQFLTPAPLARFMVGQFNSLLGHISLIDPGAG
CCEECCCCCCCEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECCCCC
VGSLTAAFVERLLANSHEVKSCFITVYEVEPIFILPLKQCLIDCCTALENKGIAANYCLY
HHHHHHHHHHHHHCCCCCHHEEEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEE
EKNFIDANSEINLPLLSTSAINFTHAIINPLYKKINNKSIEKKIISQMGIETVNLYSAFV
ECCEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHH
WLTILRLVEDGEIVAITPRSFCNGSYFRHFRKSFLEQMKLEKNPNFHKSFRSFSGI
HHHHHHHHCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6330092 [H]