BacMap

Definition	Nostoc sp. PCC 7120, complete genome.
Accession	NC_003272
Length	6,413,771

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The map label for this gene is spkB [H]

Identifier: 17230252

GI number: 17230252

Start: 3356658

End: 3357662

Strand: Reverse

Name: spkB [H]

Synonym: all2760

Alternate gene names: 17230252

Gene position: 3357662-3356658 (Counterclockwise)

Preceding gene: 17230253

Following gene: 17230248

Centisome position: 52.35

GC content: 39.3

Gene sequence:

>1005_bases
ATGGAGCTTGATGCGTTTAATAAAGGAGCAATTCCCTTAATCAATCATCCAGATTGTTCTGCTTTAGGTTATCAAGTTAT
TCAAGAACTCGGACGGAACCAAGAAGAAGGTCGCATCACTTATTTAGCTCATCATCATCAGTCTAAGCAACAGGTAGTAA
TTAAAGAATTTAGTTTTGCTCATACCTATACTGACTGGTGTGATTTTACAGCTTATGAAAGTGAAGCGAAAATCTTGCAA
AAACTAAATCATCCCCGCATTCCTCGCTATCTAGATTCTTTTGCAACTCAGACAGGTTTCTATTTAGTCCAGGAGTATAA
ACACGCTCTACCCTTAAGCTCAAAACGCAGCTTTCAGCCAGAAGAAATTAAGCAAATAGCTGTATCAGTTTTAGAAATTT
TGGTGTATTTACAGCAACGAATTGACCCAATTATTCATCGGGATATCAAACCAGAAAATATCTTAGTCGATGAGCAACTG
AATGCTTACTTAGTAGATTTTGGTTTAGCTAGGGTGCAAGATACAAAAATAGCTCTCACCAGCTTACTAACAGGAACACC
AGGCTTTATACCACCAGAGGAACAATTAGGCTATTCTTTATCTTTGGCTTCAGATTTATACAGTGTAGGGGCGACACTAA
TTTGTTTACTTGCTAATATCCGTTCTGTAGATATTGGTAAGTTGATTGACAGTAAGGGTTATTTTGATTTTCAGAAACTA
GATTCTCAAATCGATTTACGCTTCCGCTCCTGGTTAATGGGTATGGTGGAACCCAAGTGGCAATATCGCTATACTAATGC
CGCCGATGCTTTGGCAGCACTACAGCCTATTCAAGTCACTGGTAATGCTACAGCTATGGAAATATTAGCCACAGTCATCA
AGTTGAAATATCAAACTACTGTTTTGGGTTTAGCAATAATTGGGGTGCTAGCAGTCGCTGGGGCTACTTTGATACTGTCT
CAACAGGGTGGAGCCGCTCAACAGTTACAAGAGGCGAGGAAATAA

Upstream 100 bases:

>100_bases
CTCCATAGCGCGATTAAGGCAGAATGGCAGGTAATAATCTGGGTGAGTATTTATAATTTTTGGATTGCACCTTGACAAAA
TCAAACTCAGGTGGTGTGTA

Downstream 100 bases:

>100_bases
GGAATTGTGCAATTAGCGCTCTTTTAAGACAGTCGCTACACTAGTTTTTTATTTTTAGCCGTAACTGAACGGTATTGCCT
TATACCCCTATACCCCTTTC

Product: serine/threonine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MELDAFNKGAIPLINHPDCSALGYQVIQELGRNQEEGRITYLAHHHQSKQQVVIKEFSFAHTYTDWCDFTAYESEAKILQ
KLNHPRIPRYLDSFATQTGFYLVQEYKHALPLSSKRSFQPEEIKQIAVSVLEILVYLQQRIDPIIHRDIKPENILVDEQL
NAYLVDFGLARVQDTKIALTSLLTGTPGFIPPEEQLGYSLSLASDLYSVGATLICLLANIRSVDIGKLIDSKGYFDFQKL
DSQIDLRFRSWLMGMVEPKWQYRYTNAADALAALQPIQVTGNATAMEILATVIKLKYQTTVLGLAIIGVLAVAGATLILS
QQGGAAQQLQEARK

Sequences:

>Translated_334_residues
MELDAFNKGAIPLINHPDCSALGYQVIQELGRNQEEGRITYLAHHHQSKQQVVIKEFSFAHTYTDWCDFTAYESEAKILQ
KLNHPRIPRYLDSFATQTGFYLVQEYKHALPLSSKRSFQPEEIKQIAVSVLEILVYLQQRIDPIIHRDIKPENILVDEQL
NAYLVDFGLARVQDTKIALTSLLTGTPGFIPPEEQLGYSLSLASDLYSVGATLICLLANIRSVDIGKLIDSKGYFDFQKL
DSQIDLRFRSWLMGMVEPKWQYRYTNAADALAALQPIQVTGNATAMEILATVIKLKYQTTVLGLAIIGVLAVAGATLILS
QQGGAAQQLQEARK
>Mature_334_residues
MELDAFNKGAIPLINHPDCSALGYQVIQELGRNQEEGRITYLAHHHQSKQQVVIKEFSFAHTYTDWCDFTAYESEAKILQ
KLNHPRIPRYLDSFATQTGFYLVQEYKHALPLSSKRSFQPEEIKQIAVSVLEILVYLQQRIDPIIHRDIKPENILVDEQL
NAYLVDFGLARVQDTKIALTSLLTGTPGFIPPEEQLGYSLSLASDLYSVGATLICLLANIRSVDIGKLIDSKGYFDFQKL
DSQIDLRFRSWLMGMVEPKWQYRYTNAADALAALQPIQVTGNATAMEILATVIKLKYQTTVLGLAIIGVLAVAGATLILS
QQGGAAQQLQEARK

Specific function: Protein kinase required for cell motility, but not for phototaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Homo sapiens, GI4505373, Length=158, Percent_Identity=32.9113924050633, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI225735561, Length=190, Percent_Identity=30.5263157894737, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI217330634, Length=146, Percent_Identity=32.8767123287671, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI5454096, Length=200, Percent_Identity=30.5, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI41872673, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI83627721, Length=205, Percent_Identity=28.780487804878, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5453882, Length=157, Percent_Identity=31.8471337579618, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI45439359, Length=153, Percent_Identity=39.2156862745098, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI103471999, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI5031689, Length=190, Percent_Identity=32.6315789473684, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4502743, Length=204, Percent_Identity=29.9019607843137, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI14196445, Length=209, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI225637562, Length=153, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4502553, Length=156, Percent_Identity=30.1282051282051, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI41281753, Length=153, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI21361358, Length=223, Percent_Identity=28.2511210762332, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI261244925, Length=214, Percent_Identity=28.0373831775701, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI115529463, Length=185, Percent_Identity=31.3513513513514, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI261244921, Length=214, Percent_Identity=28.0373831775701, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI120659787, Length=185, Percent_Identity=31.3513513513514, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI261244927, Length=214, Percent_Identity=28.0373831775701, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI261244923, Length=214, Percent_Identity=28.0373831775701, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI19923407, Length=214, Percent_Identity=28.0373831775701, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI30039692, Length=212, Percent_Identity=25.9433962264151, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI225637560, Length=153, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI261244929, Length=214, Percent_Identity=28.0373831775701, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI222418622, Length=214, Percent_Identity=28.0373831775701, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4502023, Length=174, Percent_Identity=25.8620689655172, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI120659785, Length=150, Percent_Identity=35.3333333333333, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI19923468, Length=150, Percent_Identity=35.3333333333333, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI120659782, Length=150, Percent_Identity=35.3333333333333, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI32307163, Length=174, Percent_Identity=25.8620689655172, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI4885549, Length=174, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI163310763, Length=165, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17511015, Length=191, Percent_Identity=30.3664921465969, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539480, Length=153, Percent_Identity=30.718954248366, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508425, Length=147, Percent_Identity=31.9727891156463, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71991643, Length=158, Percent_Identity=33.5443037974684, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71991633, Length=158, Percent_Identity=33.5443037974684, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71991638, Length=158, Percent_Identity=33.5443037974684, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71991623, Length=158, Percent_Identity=33.5443037974684, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71991629, Length=158, Percent_Identity=33.5443037974684, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71991657, Length=158, Percent_Identity=33.5443037974684, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71991652, Length=158, Percent_Identity=33.5443037974684, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71991647, Length=158, Percent_Identity=33.5443037974684, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71991618, Length=158, Percent_Identity=33.5443037974684, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193208795, Length=208, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17568289, Length=225, Percent_Identity=28.8888888888889, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193208793, Length=208, Percent_Identity=28.8461538461538, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71994328, Length=233, Percent_Identity=23.6051502145923, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320715, Length=154, Percent_Identity=35.0649350649351, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324557, Length=151, Percent_Identity=32.4503311258278, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320102, Length=207, Percent_Identity=31.8840579710145, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325117, Length=92, Percent_Identity=42.3913043478261, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321126, Length=166, Percent_Identity=29.5180722891566, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325104, Length=139, Percent_Identity=30.9352517985612, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319917, Length=197, Percent_Identity=29.4416243654822, Blast_Score=66, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323643, Length=150, Percent_Identity=30, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325053, Length=207, Percent_Identity=28.0193236714976, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322682, Length=224, Percent_Identity=27.2321428571429, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319825, Length=196, Percent_Identity=29.5918367346939, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI17137760, Length=141, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922606, Length=181, Percent_Identity=32.0441988950276, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24638616, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24638612, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24638618, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17975557, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24638614, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24653972, Length=211, Percent_Identity=27.0142180094787, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24653970, Length=211, Percent_Identity=27.0142180094787, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24653974, Length=211, Percent_Identity=27.0142180094787, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653968, Length=207, Percent_Identity=26.5700483091787, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007536, Length=202, Percent_Identity=30.6930693069307, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007530, Length=202, Percent_Identity=30.6930693069307, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18858209, Length=176, Percent_Identity=30.1136363636364, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007532, Length=202, Percent_Identity=30.6930693069307, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007534, Length=202, Percent_Identity=30.6930693069307, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24653966, Length=160, Percent_Identity=30, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45553327, Length=216, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24649791, Length=249, Percent_Identity=22.8915662650602, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24647358, Length=174, Percent_Identity=28.1609195402299, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24647360, Length=174, Percent_Identity=28.1609195402299, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17737298, Length=145, Percent_Identity=33.1034482758621, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001646
- InterPro:   IPR020636
- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR017441
- InterPro:   IPR017442
- InterPro:   IPR016252 [H]

Pfam domain/function: PF00805 Pentapeptide; PF00069 Pkinase [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 37429; Mature: 37429

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00108 PROTEIN_KINASE_ST ; PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELDAFNKGAIPLINHPDCSALGYQVIQELGRNQEEGRITYLAHHHQSKQQVVIKEFSFA
CCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
HTYTDWCDFTAYESEAKILQKLNHPRIPRYLDSFATQTGFYLVQEYKHALPLSSKRSFQP
HHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EEIKQIAVSVLEILVYLQQRIDPIIHRDIKPENILVDEQLNAYLVDFGLARVQDTKIALT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
SLLTGTPGFIPPEEQLGYSLSLASDLYSVGATLICLLANIRSVDIGKLIDSKGYFDFQKL
HHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHH
DSQIDLRFRSWLMGMVEPKWQYRYTNAADALAALQPIQVTGNATAMEILATVIKLKYQTT
HHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHH
VLGLAIIGVLAVAGATLILSQQGGAAQQLQEARK
HHHHHHHHHHHHHCHHEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MELDAFNKGAIPLINHPDCSALGYQVIQELGRNQEEGRITYLAHHHQSKQQVVIKEFSFA
CCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
HTYTDWCDFTAYESEAKILQKLNHPRIPRYLDSFATQTGFYLVQEYKHALPLSSKRSFQP
HHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EEIKQIAVSVLEILVYLQQRIDPIIHRDIKPENILVDEQLNAYLVDFGLARVQDTKIALT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHH
SLLTGTPGFIPPEEQLGYSLSLASDLYSVGATLICLLANIRSVDIGKLIDSKGYFDFQKL
HHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHH
DSQIDLRFRSWLMGMVEPKWQYRYTNAADALAALQPIQVTGNATAMEILATVIKLKYQTT
HHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHH
VLGLAIIGVLAVAGATLILSQQGGAAQQLQEARK
HHHHHHHHHHHHHCHHEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12732981; 8905231 [H]