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The map label for this gene is pleD [H]

Identifier: 17229799

GI number: 17229799

Start: 2779215

End: 2780627

Strand: Direct

Name: pleD [H]

Synonym: alr2307

Alternate gene names: 17229799

Gene position: 2779215-2780627 (Clockwise)

Preceding gene: 17229798

Following gene: 17229800

Centisome position: 43.33

GC content: 39.28

Gene sequence:

>1413_bases
ATGAATTTAAATAATAACAATAAATATAAAAGCAATATTTTGGTAGTAGATGATACACCAGATAATCTGCGGCTGTTATC
AGCGATGTTAACTGCACGAGGTTTTGAAGTTCGCAAAGCCTTGAACGGTAAAATGGCACTAACTGCGTGTCAGATGCTTT
TACCTGATGTCATTTTGCTAGATGTTAGTATGCCGGATATGGATGGTTATCAAGTTTGTCAGCAACTGAAAGCTGATGAA
CACACCTCCGATATACCAGTGATATTTATTAGCGCGCTAGATGATGTCCTAGATAAAGTTAAAGCTTTCGATGTTGGTGG
TGTGGATTACATTACTAAGCCCTTTCATGGTGCGGAAGTAATCTTACGAATTGAAAATCAAATAAATTTACGTTTGTTGC
AACTTCAATTAAAAGAAAGAAATTTTTTATTGCAAGATGCCCTTAATAATTTAAAAGCCTCACAGGTTCAACAAATTCAA
AACGAGAAAATGGTGGCACTTGGACAATTAGTAGCAGGTATTGCCCATGAAGTTAATAACCCTATTAGTTTTATCTATGG
CAACCTGCAATATGCTAATGAGTATATCCAAGATTTAGTGAAAATCATCGAAGTTTATCAACAGGAGTATCCCAATCCAA
CATCCAAAATTCAACAACTAATTCAAAAGACAGACTTGAGTTTTGTGATGCAAGACCTGCAAAACTTAATGGGTGCTATG
TACAGAGGATCTGATCGGATTCGGGAAATTGTACTGGCGCTGCAAAACTTTTCCCGCCATGACGAGGCGCAAATGAAATT
GGTAAACATTCATCAGGGAATAGACAGCGCCTTGGTGATGTTACAGCATCGACTCAATGCCACATCAAATCGTCCAGCAA
TTGATGTGGTGAAAGATTACAGTAACTTACCTTTGATTACTTGCTATCCGAGCGAATTAAATCAAGTATTTATGCACATC
TTAAATAATGCCATAGATGCTTTGGCGGTGAGAGAAGAAACTGATTCTGGCGTAATACCAATGACTCCCAGACTACGTGT
ACAAAATAGCCAAGTCAATCCTCTGTGGGTAAGTGTTCGTCAAGGAACAGCTCATCTACAGTCATTGGGAATAACCAAAA
TCAAGGGAGAACCCCCAAACCCAAATCCTCAAATTCGGATTCGCACAGAATTACTCAACTCGCACACCGTGAGGATTGCG
ATCGCCGATAATGGTGTAGGTATAGATCAATCTTGGCAATCCCATCTGTTTGACCCATTTTTCACCACAAAAACCGTAGG
CAAAGGTAGTGGATTGGGATTATCTATCAGCTATCAGATTATAGTGCAGAAACACCAAGGGCAAATCACCTGTGCTTCTG
CATTAGGCGAAGGAGCAGAGTTTGTTATTACAATTCCGATTACACCAGTCTGA

Upstream 100 bases:

>100_bases
TCAACAAAATATTTGTTGAGTTTAGGTAATTTTTATATAAGTCCGTCCAAACAGCATTTAATATATTTATCAGGGGATAA
ATGATGAATTTTAATTCATA

Downstream 100 bases:

>100_bases
GTAGAAAGGGCTGAGTCAAATGACACTACAAATAAATTAGGATTTTTAGTCAACACAGGCTACTATTTTCATTCACATAA
AAGCATAATAGAAGTGTGAC

Product: two-component hybrid sensor and regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNLNNNNKYKSNILVVDDTPDNLRLLSAMLTARGFEVRKALNGKMALTACQMLLPDVILLDVSMPDMDGYQVCQQLKADE
HTSDIPVIFISALDDVLDKVKAFDVGGVDYITKPFHGAEVILRIENQINLRLLQLQLKERNFLLQDALNNLKASQVQQIQ
NEKMVALGQLVAGIAHEVNNPISFIYGNLQYANEYIQDLVKIIEVYQQEYPNPTSKIQQLIQKTDLSFVMQDLQNLMGAM
YRGSDRIREIVLALQNFSRHDEAQMKLVNIHQGIDSALVMLQHRLNATSNRPAIDVVKDYSNLPLITCYPSELNQVFMHI
LNNAIDALAVREETDSGVIPMTPRLRVQNSQVNPLWVSVRQGTAHLQSLGITKIKGEPPNPNPQIRIRTELLNSHTVRIA
IADNGVGIDQSWQSHLFDPFFTTKTVGKGSGLGLSISYQIIVQKHQGQITCASALGEGAEFVITIPITPV

Sequences:

>Translated_470_residues
MNLNNNNKYKSNILVVDDTPDNLRLLSAMLTARGFEVRKALNGKMALTACQMLLPDVILLDVSMPDMDGYQVCQQLKADE
HTSDIPVIFISALDDVLDKVKAFDVGGVDYITKPFHGAEVILRIENQINLRLLQLQLKERNFLLQDALNNLKASQVQQIQ
NEKMVALGQLVAGIAHEVNNPISFIYGNLQYANEYIQDLVKIIEVYQQEYPNPTSKIQQLIQKTDLSFVMQDLQNLMGAM
YRGSDRIREIVLALQNFSRHDEAQMKLVNIHQGIDSALVMLQHRLNATSNRPAIDVVKDYSNLPLITCYPSELNQVFMHI
LNNAIDALAVREETDSGVIPMTPRLRVQNSQVNPLWVSVRQGTAHLQSLGITKIKGEPPNPNPQIRIRTELLNSHTVRIA
IADNGVGIDQSWQSHLFDPFFTTKTVGKGSGLGLSISYQIIVQKHQGQITCASALGEGAEFVITIPITPV
>Mature_470_residues
MNLNNNNKYKSNILVVDDTPDNLRLLSAMLTARGFEVRKALNGKMALTACQMLLPDVILLDVSMPDMDGYQVCQQLKADE
HTSDIPVIFISALDDVLDKVKAFDVGGVDYITKPFHGAEVILRIENQINLRLLQLQLKERNFLLQDALNNLKASQVQQIQ
NEKMVALGQLVAGIAHEVNNPISFIYGNLQYANEYIQDLVKIIEVYQQEYPNPTSKIQQLIQKTDLSFVMQDLQNLMGAM
YRGSDRIREIVLALQNFSRHDEAQMKLVNIHQGIDSALVMLQHRLNATSNRPAIDVVKDYSNLPLITCYPSELNQVFMHI
LNNAIDALAVREETDSGVIPMTPRLRVQNSQVNPLWVSVRQGTAHLQSLGITKIKGEPPNPNPQIRIRTELLNSHTVRIA
IADNGVGIDQSWQSHLFDPFFTTKTVGKGSGLGLSISYQIIVQKHQGQITCASALGEGAEFVITIPITPV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=323, Percent_Identity=28.7925696594427, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=38.0952380952381, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=35.0877192982456, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=32.4786324786325, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788394, Length=172, Percent_Identity=28.4883720930233, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786911, Length=114, Percent_Identity=34.2105263157895, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=31.404958677686, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789809, Length=113, Percent_Identity=31.858407079646, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=113, Percent_Identity=31.858407079646, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=35.5769230769231, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786784, Length=151, Percent_Identity=28.476821192053, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 52507; Mature: 52507

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNNNNKYKSNILVVDDTPDNLRLLSAMLTARGFEVRKALNGKMALTACQMLLPDVILL
CCCCCCCCEECEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCEEEE
DVSMPDMDGYQVCQQLKADEHTSDIPVIFISALDDVLDKVKAFDVGGVDYITKPFHGAEV
EECCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEE
ILRIENQINLRLLQLQLKERNFLLQDALNNLKASQVQQIQNEKMVALGQLVAGIAHEVNN
EEEECCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
PISFIYGNLQYANEYIQDLVKIIEVYQQEYPNPTSKIQQLIQKTDLSFVMQDLQNLMGAM
CCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRGSDRIREIVLALQNFSRHDEAQMKLVNIHQGIDSALVMLQHRLNATSNRPAIDVVKDY
HCCHHHHHHHHHHHHHCCCCCHHHEEEHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHCC
SNLPLITCYPSELNQVFMHILNNAIDALAVREETDSGVIPMTPRLRVQNSQVNPLWVSVR
CCCCEEEECHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCCEEEECCCCCEEEEEEE
QGTAHLQSLGITKIKGEPPNPNPQIRIRTELLNSHTVRIAIADNGVGIDQSWQSHLFDPF
CCHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHCCCCC
FTTKTVGKGSGLGLSISYQIIVQKHQGQITCASALGEGAEFVITIPITPV
HHHCCCCCCCCCEEEEEEEEEEEECCCCEEEHHHCCCCCEEEEEEECCCC
>Mature Secondary Structure
MNLNNNNKYKSNILVVDDTPDNLRLLSAMLTARGFEVRKALNGKMALTACQMLLPDVILL
CCCCCCCCEECEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCCEEEE
DVSMPDMDGYQVCQQLKADEHTSDIPVIFISALDDVLDKVKAFDVGGVDYITKPFHGAEV
EECCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEE
ILRIENQINLRLLQLQLKERNFLLQDALNNLKASQVQQIQNEKMVALGQLVAGIAHEVNN
EEEECCCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
PISFIYGNLQYANEYIQDLVKIIEVYQQEYPNPTSKIQQLIQKTDLSFVMQDLQNLMGAM
CCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRGSDRIREIVLALQNFSRHDEAQMKLVNIHQGIDSALVMLQHRLNATSNRPAIDVVKDY
HCCHHHHHHHHHHHHHCCCCCHHHEEEHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHCC
SNLPLITCYPSELNQVFMHILNNAIDALAVREETDSGVIPMTPRLRVQNSQVNPLWVSVR
CCCCEEEECHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCCEEEECCCCCEEEEEEE
QGTAHLQSLGITKIKGEPPNPNPQIRIRTELLNSHTVRIAIADNGVGIDQSWQSHLFDPF
CCHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHCCCCC
FTTKTVGKGSGLGLSISYQIIVQKHQGQITCASALGEGAEFVITIPITPV
HHHCCCCCCCCCEEEEEEEEEEEECCCCEEEHHHCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA