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The map label for this gene is amy [H]

Identifier: 17228159

GI number: 17228159

Start: 767105

End: 769003

Strand: Direct

Name: amy [H]

Synonym: alr0663

Alternate gene names: 17228159

Gene position: 767105-769003 (Clockwise)

Preceding gene: 17228154

Following gene: 17228164

Centisome position: 11.96

GC content: 39.23

Gene sequence:

>1899_bases
ATGCTTAACTCAATTTACTCCAGAGAAGTTTATGATATCTTCAATCAGGCTAAATGTAATGCTCTGAACAAAGCCACGAA
AAGTGTCCAAGTTGGCGATGAGGTGAAAGAAATTCCTTCGCCTTTTCCATCACCAGCCGATTGGCGCGATCTATGGATTT
ACTTTATTATGACAGACCGCTTTAATAATCCATCTGCGCCACCTACAATGTCTTGGAATGCTTTTGATGGTAATGATTTT
TTAGGTAAAAAAGCTTTGGGTTTTCAAGGTGGTACTTTTGAAGGTATCCGTCAACAATTAGGATACTTAGAAAAATTAGG
AGTAGGAGCGATTTGGATCACTCCTCCTTTCAAAAATTGCCAGTATGAACCTACATACCACGGCTATGGAATTCAAGATT
TTTTACAAATTGATCCACGATTTGCTAGTAACAAAGATAATCCAGAAGCAGAATTACAAGCACTAATTGATGAGGCTCAT
GCACGCGGTATTTATGTGATTTTTGATATTGTACTTAACCATACAGGTAACGTTTTCAGTTATTTTATTGAGGGCAAAAA
TGTCAAATCAACTGAGACCGACAGAGATCTGCCACTACTCCCATATGATATTAAATGGCATAATAAAACAGGCGAGCCAC
AGTGGATAAATGCACCAGATGATCAAGATCCTGACCTTCATCCTAATGCAGCAGTATGGCCTAGAGAACTTTGTAAAAAT
GAGTATTTTCGCAGGAGAGGCACTCGAAAAGGTGATACTGGGGAAGGCGGAGATTTTACTGATTTGCGAGAGCTATTGAC
TGAGAAAGAAGAAGTACGTAATATCTTAATTCGGATTCATCAATATTTAATTGCCAAGTTTGATATTGACGGATTTCGGA
TTGATACTTTGCGATTTATTGAGCCAGAGTTCGCCAGAATTTTTGGTAATTCCATGCATGAATTTGCTTTAAGCATTGGT
AAGAAAAATTTCTTTAGCTTTGGTGAGGTATGGGCTGACGAAGACAAGACAGAGAAGAAAATTAGCCAATTTATTGGTCG
TAAAGCGACGGAACCAGGTGATTTATTAGGTATTGATGCAGCACTTGATTTCCCCTTATTCTTCAAACTCCGTTATGTGT
TGAAAGGATTACAACCAATAAAAGAACTAGCTGAGGTCTATGAAAAGCGTAAGGAAAGTCTGCGCGGTATTATCAGTTCT
CATGGTGAAGTTAGCAAATTTCTGGTGACATTCCTTGATAATCATGATTGGAAGAGACGCTTTTACTACAGTAACCCAGC
AAATCCTCAAAAGTTTGATGACCAACTAACTTTAGGGATTACCTGTTTGTTTGCCTTACAAGGGATACCCTGCATATACT
ACGGCACAGAACAGGGACTCAATGGTTATCTTACCGAAAATCGCGATTTTGGGGATCTTGTAGTTCGTGAAGCTTTATGG
GGTAAACCAGGCGGCGGCTTTAAGCAAGACCACCCGTTCTATAAAACAATTGCAAAGTTATCAGAATACCGCAATAGCCA
TCCGGCACTGCGTTATGGAAGGCAGTATTTTCGTCCTATATCTGGCGATCGCATTAATTATGCCATTTCTCCCTACCCGT
CTGGTGTCCTGGCGTTCTCTCGCATCTTGAATGAGACAGAAGTGGTTGTGGTAGCAAATACGAACACAGAATTTACTCAG
TCTGTTTATGTAATAGTTGATGACAATCTCCATTCTGAAAACCCTACTTTCAAGATTTTATTTAGCAATAAATCTAGGGA
TAACAGTATTCAACCGGAAAAAGTTGAAGAACATCACGGTGTCAATATCACCGAAGTTAATGGTCACAAGAATTATGGGC
CAGTACGAGTGATGAAAGTAACTCTCCAACCGATGGAAGTACAAATTTTAGTGAAATAG

Upstream 100 bases:

>100_bases
GTGAGTTTACTAAGTGTATCTTTTTAGCAACTGCATAAGCTCACAATACAGGTCTCTATTTAGTTACAGAAATAATATCT
TACATATCTAAGGAGAGAGA

Downstream 100 bases:

>100_bases
GAATAACATATTTGAGACTCTCAATTCGTGAAAGTCTCAAATATGTTACTTCATCATTGTTGGGCGATGGGGCGTATCCC
GTCGCATAAACTTTTTTCAT

Product: hypothetical protein

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MLNSIYSREVYDIFNQAKCNALNKATKSVQVGDEVKEIPSPFPSPADWRDLWIYFIMTDRFNNPSAPPTMSWNAFDGNDF
LGKKALGFQGGTFEGIRQQLGYLEKLGVGAIWITPPFKNCQYEPTYHGYGIQDFLQIDPRFASNKDNPEAELQALIDEAH
ARGIYVIFDIVLNHTGNVFSYFIEGKNVKSTETDRDLPLLPYDIKWHNKTGEPQWINAPDDQDPDLHPNAAVWPRELCKN
EYFRRRGTRKGDTGEGGDFTDLRELLTEKEEVRNILIRIHQYLIAKFDIDGFRIDTLRFIEPEFARIFGNSMHEFALSIG
KKNFFSFGEVWADEDKTEKKISQFIGRKATEPGDLLGIDAALDFPLFFKLRYVLKGLQPIKELAEVYEKRKESLRGIISS
HGEVSKFLVTFLDNHDWKRRFYYSNPANPQKFDDQLTLGITCLFALQGIPCIYYGTEQGLNGYLTENRDFGDLVVREALW
GKPGGGFKQDHPFYKTIAKLSEYRNSHPALRYGRQYFRPISGDRINYAISPYPSGVLAFSRILNETEVVVVANTNTEFTQ
SVYVIVDDNLHSENPTFKILFSNKSRDNSIQPEKVEEHHGVNITEVNGHKNYGPVRVMKVTLQPMEVQILVK

Sequences:

>Translated_632_residues
MLNSIYSREVYDIFNQAKCNALNKATKSVQVGDEVKEIPSPFPSPADWRDLWIYFIMTDRFNNPSAPPTMSWNAFDGNDF
LGKKALGFQGGTFEGIRQQLGYLEKLGVGAIWITPPFKNCQYEPTYHGYGIQDFLQIDPRFASNKDNPEAELQALIDEAH
ARGIYVIFDIVLNHTGNVFSYFIEGKNVKSTETDRDLPLLPYDIKWHNKTGEPQWINAPDDQDPDLHPNAAVWPRELCKN
EYFRRRGTRKGDTGEGGDFTDLRELLTEKEEVRNILIRIHQYLIAKFDIDGFRIDTLRFIEPEFARIFGNSMHEFALSIG
KKNFFSFGEVWADEDKTEKKISQFIGRKATEPGDLLGIDAALDFPLFFKLRYVLKGLQPIKELAEVYEKRKESLRGIISS
HGEVSKFLVTFLDNHDWKRRFYYSNPANPQKFDDQLTLGITCLFALQGIPCIYYGTEQGLNGYLTENRDFGDLVVREALW
GKPGGGFKQDHPFYKTIAKLSEYRNSHPALRYGRQYFRPISGDRINYAISPYPSGVLAFSRILNETEVVVVANTNTEFTQ
SVYVIVDDNLHSENPTFKILFSNKSRDNSIQPEKVEEHHGVNITEVNGHKNYGPVRVMKVTLQPMEVQILVK
>Mature_632_residues
MLNSIYSREVYDIFNQAKCNALNKATKSVQVGDEVKEIPSPFPSPADWRDLWIYFIMTDRFNNPSAPPTMSWNAFDGNDF
LGKKALGFQGGTFEGIRQQLGYLEKLGVGAIWITPPFKNCQYEPTYHGYGIQDFLQIDPRFASNKDNPEAELQALIDEAH
ARGIYVIFDIVLNHTGNVFSYFIEGKNVKSTETDRDLPLLPYDIKWHNKTGEPQWINAPDDQDPDLHPNAAVWPRELCKN
EYFRRRGTRKGDTGEGGDFTDLRELLTEKEEVRNILIRIHQYLIAKFDIDGFRIDTLRFIEPEFARIFGNSMHEFALSIG
KKNFFSFGEVWADEDKTEKKISQFIGRKATEPGDLLGIDAALDFPLFFKLRYVLKGLQPIKELAEVYEKRKESLRGIISS
HGEVSKFLVTFLDNHDWKRRFYYSNPANPQKFDDQLTLGITCLFALQGIPCIYYGTEQGLNGYLTENRDFGDLVVREALW
GKPGGGFKQDHPFYKTIAKLSEYRNSHPALRYGRQYFRPISGDRINYAISPYPSGVLAFSRILNETEVVVVANTNTEFTQ
SVYVIVDDNLHSENPTFKILFSNKSRDNSIQPEKVEEHHGVNITEVNGHKNYGPVRVMKVTLQPMEVQILVK

Specific function: Since Only Maltooligosaccharides Up To A Chain Length Of 6 Glucose Units Are Actively Transported Through The Cytoplasmic Membrane VIa The Membrane-Bound Complex Of Three Proteins, Malf, Malg, And Malk, Longer Maltooligosaccharides Must First Be Degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=342, Percent_Identity=25.7309941520468, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1790687, Length=407, Percent_Identity=25.0614250614251, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=100, Percent_Identity=36, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005323 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF03714 PUD [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 72536; Mature: 72536

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSIYSREVYDIFNQAKCNALNKATKSVQVGDEVKEIPSPFPSPADWRDLWIYFIMTDR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEEEEEEC
FNNPSAPPTMSWNAFDGNDFLGKKALGFQGGTFEGIRQQLGYLEKLGVGAIWITPPFKNC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
QYEPTYHGYGIQDFLQIDPRFASNKDNPEAELQALIDEAHARGIYVIFDIVLNHTGNVFS
CCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCEEE
YFIEGKNVKSTETDRDLPLLPYDIKWHNKTGEPQWINAPDDQDPDLHPNAAVWPRELCKN
EEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
EYFRRRGTRKGDTGEGGDFTDLRELLTEKEEVRNILIRIHQYLIAKFDIDGFRIDTLRFI
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
EPEFARIFGNSMHEFALSIGKKNFFSFGEVWADEDKTEKKISQFIGRKATEPGDLLGIDA
CHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEHH
ALDFPLFFKLRYVLKGLQPIKELAEVYEKRKESLRGIISSHGEVSKFLVTFLDNHDWKRR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEE
FYYSNPANPQKFDDQLTLGITCLFALQGIPCIYYGTEQGLNGYLTENRDFGDLVVREALW
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHC
GKPGGGFKQDHPFYKTIAKLSEYRNSHPALRYGRQYFRPISGDRINYAISPYPSGVLAFS
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
RILNETEVVVVANTNTEFTQSVYVIVDDNLHSENPTFKILFSNKSRDNSIQPEKVEEHHG
HHCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCC
VNITEVNGHKNYGPVRVMKVTLQPMEVQILVK
CEEEEECCCCCCCCEEEEEEEECCEEEEEEEC
>Mature Secondary Structure
MLNSIYSREVYDIFNQAKCNALNKATKSVQVGDEVKEIPSPFPSPADWRDLWIYFIMTDR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEEEEEEC
FNNPSAPPTMSWNAFDGNDFLGKKALGFQGGTFEGIRQQLGYLEKLGVGAIWITPPFKNC
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
QYEPTYHGYGIQDFLQIDPRFASNKDNPEAELQALIDEAHARGIYVIFDIVLNHTGNVFS
CCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCEEE
YFIEGKNVKSTETDRDLPLLPYDIKWHNKTGEPQWINAPDDQDPDLHPNAAVWPRELCKN
EEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
EYFRRRGTRKGDTGEGGDFTDLRELLTEKEEVRNILIRIHQYLIAKFDIDGFRIDTLRFI
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
EPEFARIFGNSMHEFALSIGKKNFFSFGEVWADEDKTEKKISQFIGRKATEPGDLLGIDA
CHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEHH
ALDFPLFFKLRYVLKGLQPIKELAEVYEKRKESLRGIISSHGEVSKFLVTFLDNHDWKRR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEE
FYYSNPANPQKFDDQLTLGITCLFALQGIPCIYYGTEQGLNGYLTENRDFGDLVVREALW
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHC
GKPGGGFKQDHPFYKTIAKLSEYRNSHPALRYGRQYFRPISGDRINYAISPYPSGVLAFS
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHH
RILNETEVVVVANTNTEFTQSVYVIVDDNLHSENPTFKILFSNKSRDNSIQPEKVEEHHG
HHCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCC
VNITEVNGHKNYGPVRVMKVTLQPMEVQILVK
CEEEEECCCCCCCCEEEEEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8424949; 8485150 [H]