Click here to switch to the map view.

The map label for this gene is rph [H]

Identifier: 17227565

GI number: 17227565

Start: 73290

End: 74033

Strand: Direct

Name: rph [H]

Synonym: alr0069

Alternate gene names: 17227565

Gene position: 73290-74033 (Clockwise)

Preceding gene: 17227564

Following gene: 17227568

Centisome position: 1.14

GC content: 43.68

Gene sequence:

>744_bases
ATGGTTTGGCAACGTCCCGACGGTAGAAAACCCTATGAACTCCGTCCTATAAATTTTCATACTAAATTTACTCGTTTTGC
CCCTGGTTCGGTGCTGACAATATGCGGTGAGACTAAGGTACTGTGTACTGTTAGCGTTGCGGAAAGTGTACCCAAGTTTC
TTACAGGTAGTGGCAAAGGTTGGTTAACGGCTGAGTATCGGATGCTACCATCAGCTACACAGCAACGGCATGAGAGAGAA
TTATTAAAGTTATCTGGACGGACGCAAGAAATTCAACGTTTGATTGGGCGTAGTTTACGAGCAGCGTTAGATTTTGAGGC
GTTGGGAGAACGGACTTTAACTGTAGATGCAGATGTGTTACAAGCCGATGCAGGTACAAGAACGGCAGCAATTACAGGCG
GATTTGTAGCATTAGCTGAGGCTATTTCTCAATTATTGCAGCGAGGGGTATTAGAGCGATCGCCTCTCTGCGGACAAATC
GCCGCCGTTTCTGTGGGTTTATTAGAACAAGAAGCATATTTAGATTTGAACTATATAGAAGATGTAGCCGCAACGGTAGA
TTTTAACGTTGTGATGAATAAAAACTTAGGAATTATCGAAGTTCAGGGAACAGCAGAAGAAGGCAGTTTTAGCCGTACCC
AGTTGAATCAGCTACTAGATTGCGCCGAAACAGGAATTCAGCAACTATTAATTGCCCAACAGCAAGCAATTACTGATTGG
GATAGGTTGTTTGTTGGCAAATAG

Upstream 100 bases:

>100_bases
TGTAGGCATGAGATCACAACCTCACACAGTACTTAATGTTTTGCACCACTAAGGTAACTTTAAAGGGGTATTCTACAATT
AAAAGTTAGAGGCAACTCTC

Downstream 100 bases:

>100_bases
AAAGTGGGCTTCCGCCGTGAGCGTGAGCGTTCAACTGAGCGCTCACGCCGTTCGCGTAGCGTCCCGCCGGGAAGTCCCAA
CGGGAATAGGGATATTAGTC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLTGSGKGWLTAEYRMLPSATQQRHERE
LLKLSGRTQEIQRLIGRSLRAALDFEALGERTLTVDADVLQADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQI
AAVSVGLLEQEAYLDLNYIEDVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQQAITDW
DRLFVGK

Sequences:

>Translated_247_residues
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLTGSGKGWLTAEYRMLPSATQQRHERE
LLKLSGRTQEIQRLIGRSLRAALDFEALGERTLTVDADVLQADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQI
AAVSVGLLEQEAYLDLNYIEDVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQQAITDW
DRLFVGK
>Mature_247_residues
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLTGSGKGWLTAEYRMLPSATQQRHERE
LLKLSGRTQEIQRLIGRSLRAALDFEALGERTLTVDADVLQADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQI
AAVSVGLLEQEAYLDLNYIEDVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQQAITDW
DRLFVGK

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=55.2380952380952, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27152; Mature: 27152

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLTGSGKG
CCCCCCCCCCCCEEEECCCCCCEEEECCCCEEEEECCCEEEEEEEHHHHHHHHHCCCCCC
WLTAEYRMLPSATQQRHERELLKLSGRTQEIQRLIGRSLRAALDFEALGERTLTVDADVL
EEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEEEHHHH
QADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQIAAVSVGLLEQEAYLDLNYIE
HCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
DVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQQAITDW
HHHHHEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRLFVGK
HHHHCCC
>Mature Secondary Structure
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLTGSGKG
CCCCCCCCCCCCEEEECCCCCCEEEECCCCEEEEECCCEEEEEEEHHHHHHHHHCCCCCC
WLTAEYRMLPSATQQRHERELLKLSGRTQEIQRLIGRSLRAALDFEALGERTLTVDADVL
EEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEEEHHHH
QADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQIAAVSVGLLEQEAYLDLNYIE
HCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
DVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQQAITDW
HHHHHEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRLFVGK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA