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The map label for this gene is acnB [H]

Identifier: 172087756

GI number: 172087756

Start: 2415543

End: 2418083

Strand: Reverse

Name: acnB [H]

Synonym: VF_2158

Alternate gene names: 172087756

Gene position: 2418083-2415543 (Counterclockwise)

Preceding gene: 59712766

Following gene: 59712761

Centisome position: 83.45

GC content: 44.04

Gene sequence:

>2541_bases
GTGGTCCCAAGACCACTGGATGCTGAGCAAGTAGCAGCATTAATTGAATTAGTAAAAAATCCGCCTCAAGGCGAAGAAGA
AGTTATTCTTGATTTACTAGAGAATCGTATTCCCCCAGGCGTTGATGAAGCAGCTTATGTTAAAGCTGGCTTCTTAACAG
CAGTTACTAAAGGAGAAGTATCTTCTCCACTTGTTAGTAAAGAAAAAGCAGCAGAATTACTTGGCACAATGCAAGGTGGT
TATAATATAGAGCCTCTTGTTTCTTTATTAGATGACACTGAACTGGCTCCTATTGCAGTTAAAGCGCTTTCTCATACACT
TTTGATGTTTGATGCTTTTTATGACGTAGAAGAAAAAGCGAAAGCGGGTAACAGTTCTGCGCAGCAAGTACTTCAATCAT
GGGCTGATGCAGAATGGTTTACATCAAAAGATAAAGTAGCTGAAAAGATCACAGTAAAAGTATTTAAAGTCACAGGTGAA
ACGAACACCGATGATCTTTCTCCTGCTCCTGATGCATGGTCTCGTCCTGATATTCCTGTACATGCAAAAGCAATGTTGAA
AATGGAACGTGATGGTATCACTCCAGATGAACAGGGTAGCGTTGGTCCTATTACTCAAATCGAAGAAATGCAAAAAGACG
GCATCCCTTTAGCTTATGTTGGTGATGTTGTTGGTACTGGTTCTTCTCGCAAATCAGCGACTAACTCAGTGCTTTGGTTC
ATGGGTGAAGATATCCCATTTGTACCAAACAAGCGCACTGGCGGTGTTTGTCTTGGTGGTAAGATCGCACCAATTTTCTA
TAACACGATGGAAGATTCAGGAGCACTACCGATTGAACTTGATGTTCAAAATATGAATATGGGAGATATTATTGATATTT
TCCCTTATGAAGGTGTAGTTCGTAAAAATAATGAAGAGATCTCTAGCTTTAAATTGAGCGCAGTGTTGCTTGATGAAGTT
CGTGCTGGAGGTCGTATTCCACTGATTATTGGTCGTGGATTAACAAGCCGTGCACGTATTTCTCTTGGTTTAGAGGAAAC
TGACTTATTTGCTAAGCCAATTGATCCATCTGCATCAGATAAAGGCTACACACTAGCTCAGAAGATGGTTGGTAAAGCAT
GTGGTGTTGAAGGCGTTCGTGCAGGTCAATATTGTGAGCCTAAGATGACTACGGTAGGCTCTCAGGATACGACGGGTCCT
ATGACGCGTGATGAGCTTAAAGATCTTGCATGTCTTGGTTTCTCTGCGGATCTTGTGATGCAATCTTTCTGTCACACATC
GGCATACCCGAAACCAGTTGATGTTAACACGCACCACACGCTACCTGATTTCATCATGAACCGTGCAGGTGTTTCACTGC
GTCCGGGTGATGGTGTTATCCACTCATGGTTAAACCGTATGCTCCTACCTGATACTGTTGGTACTGGTGGTGACTCGCAT
ACTCGTTTCCCTCTAGGTATTTCGTTCCCTGCTGGTTCTGGTCTAGTAGCATTTGCGGCAGCAACAGGTGTTATGCCTCT
TGATATGCCTGAATCTATCTTGGTTCGCTTTAAAGGTGAAATGCAGCCAGGTATCACGCTACGTGACCTAGTACACGCGA
TTCCTTTATACGGTATCAAGCAAGGCCTACTAACAGTAGAGAAAGCAGGTAAGATCAACGAATTCTCTGGTCGAGTACTA
GAAATTGAAGGTGTTGAACACCTATCTGTTGAGCAAGCGTTTGAACTTTCTGATGCATCTGCTGAGCGTTCAGCTGCTGG
TTGTACAGTTAAGTTGTCTCAAGAGTCCATTGAAGAATATTTGAATTCAAACATCGTTATGCTTAAGTGGATGATCGCTG
AAGGCTACGGTGATGTTCGTACTATCGAGCGTCGTATTACGGCAATGGAAGAGTGGCTAGCGAATCCTGAGTTGCTGTCT
GCTGATTCAGATGCTGAATACGCTCACGTTATCGAAATCGACCTTGCTGACATTGATCAACCAATCCTATGTGCACCAAA
CGATCCAGATGATGCCCGTCTTCTTTCTGACGTTCAAGGCACAGAGATCCAAGAAGTGTTCATCGGTTCTTGTATGACCA
ACATCGGTCACTTCCGTGCTGCAGGTAAGATGCTTGAAGAGTTCAACGGCTCGCTGAGCACTCGTCTGTGGGTGGCTCCG
CCAACTAAGATGGATAAAGATCAGCTAACAGAAGAAGGCTACTACGGCATCTTCGGCCGTGCTGGAGTTCGTATTGAAAC
TCCGGGTTGTTCACTATGTATGGGTAATCAGGCACGTGTGGCAGACAAGTCGACAGTAATGTCTACGTCTACTCGTAACT
TCCCGAACCGTTTGGGTACAGGTGCGAATGTTTACCTAGCATCTGCTGAACTTTCTGCGGTTGGTGCAATTCTTGGTCGT
ATCCCGACGAAGGAAGAGTATCTAGAGTACGCTGATAAGATTAATGCAACCGCTGCAGATACCTACCGTTATCTGAACTT
CCATAAAATGGGAGCATATACAGAGAAAGCGGATACGGTTATTTTCCAAGAGCCAGCTTAA

Upstream 100 bases:

>100_bases
TTCCAAGGATGCTAACTTATCAAGAGTTAGCCAAAATGTGAGGAATTAAGTCGTGCTTGAAGCTTATCGTAAACACGTCG
AAGAACGTGCTTCAGAAGGC

Downstream 100 bases:

>100_bases
CCTTATCTTCATAAATACAAAAAGCAGCCAATTGGCTGCTTTTTTATTTAGAGGGCTGGATACTTACCTATTTCATTGAA
TATATTGTTGCAACATTACG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 846; Mature: 846

Protein sequence:

>846_residues
MVPRPLDAEQVAALIELVKNPPQGEEEVILDLLENRIPPGVDEAAYVKAGFLTAVTKGEVSSPLVSKEKAAELLGTMQGG
YNIEPLVSLLDDTELAPIAVKALSHTLLMFDAFYDVEEKAKAGNSSAQQVLQSWADAEWFTSKDKVAEKITVKVFKVTGE
TNTDDLSPAPDAWSRPDIPVHAKAMLKMERDGITPDEQGSVGPITQIEEMQKDGIPLAYVGDVVGTGSSRKSATNSVLWF
MGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELDVQNMNMGDIIDIFPYEGVVRKNNEEISSFKLSAVLLDEV
RAGGRIPLIIGRGLTSRARISLGLEETDLFAKPIDPSASDKGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQDTTGP
MTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSH
TRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPLYGIKQGLLTVEKAGKINEFSGRVL
EIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQESIEEYLNSNIVMLKWMIAEGYGDVRTIERRITAMEEWLANPELLS
ADSDAEYAHVIEIDLADIDQPILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNIGHFRAAGKMLEEFNGSLSTRLWVAP
PTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKSTVMSTSTRNFPNRLGTGANVYLASAELSAVGAILGR
IPTKEEYLEYADKINATAADTYRYLNFHKMGAYTEKADTVIFQEPA

Sequences:

>Translated_846_residues
MVPRPLDAEQVAALIELVKNPPQGEEEVILDLLENRIPPGVDEAAYVKAGFLTAVTKGEVSSPLVSKEKAAELLGTMQGG
YNIEPLVSLLDDTELAPIAVKALSHTLLMFDAFYDVEEKAKAGNSSAQQVLQSWADAEWFTSKDKVAEKITVKVFKVTGE
TNTDDLSPAPDAWSRPDIPVHAKAMLKMERDGITPDEQGSVGPITQIEEMQKDGIPLAYVGDVVGTGSSRKSATNSVLWF
MGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELDVQNMNMGDIIDIFPYEGVVRKNNEEISSFKLSAVLLDEV
RAGGRIPLIIGRGLTSRARISLGLEETDLFAKPIDPSASDKGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQDTTGP
MTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSH
TRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPLYGIKQGLLTVEKAGKINEFSGRVL
EIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQESIEEYLNSNIVMLKWMIAEGYGDVRTIERRITAMEEWLANPELLS
ADSDAEYAHVIEIDLADIDQPILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNIGHFRAAGKMLEEFNGSLSTRLWVAP
PTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKSTVMSTSTRNFPNRLGTGANVYLASAELSAVGAILGR
IPTKEEYLEYADKINATAADTYRYLNFHKMGAYTEKADTVIFQEPA
>Mature_846_residues
MVPRPLDAEQVAALIELVKNPPQGEEEVILDLLENRIPPGVDEAAYVKAGFLTAVTKGEVSSPLVSKEKAAELLGTMQGG
YNIEPLVSLLDDTELAPIAVKALSHTLLMFDAFYDVEEKAKAGNSSAQQVLQSWADAEWFTSKDKVAEKITVKVFKVTGE
TNTDDLSPAPDAWSRPDIPVHAKAMLKMERDGITPDEQGSVGPITQIEEMQKDGIPLAYVGDVVGTGSSRKSATNSVLWF
MGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELDVQNMNMGDIIDIFPYEGVVRKNNEEISSFKLSAVLLDEV
RAGGRIPLIIGRGLTSRARISLGLEETDLFAKPIDPSASDKGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQDTTGP
MTRDELKDLACLGFSADLVMQSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSH
TRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPLYGIKQGLLTVEKAGKINEFSGRVL
EIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQESIEEYLNSNIVMLKWMIAEGYGDVRTIERRITAMEEWLANPELLS
ADSDAEYAHVIEIDLADIDQPILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNIGHFRAAGKMLEEFNGSLSTRLWVAP
PTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKSTVMSTSTRNFPNRLGTGANVYLASAELSAVGAILGR
IPTKEEYLEYADKINATAADTYRYLNFHKMGAYTEKADTVIFQEPA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=846, Percent_Identity=77.8959810874704, Blast_Score=1358, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=377, Percent_Identity=27.3209549071618, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323335, Length=380, Percent_Identity=25.7894736842105, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 91666; Mature: 91666

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPRPLDAEQVAALIELVKNPPQGEEEVILDLLENRIPPGVDEAAYVKAGFLTAVTKGEV
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEECCCC
SSPLVSKEKAAELLGTMQGGYNIEPLVSLLDDTELAPIAVKALSHTLLMFDAFYDVEEKA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHH
KAGNSSAQQVLQSWADAEWFTSKDKVAEKITVKVFKVTGETNTDDLSPAPDAWSRPDIPV
HCCCHHHHHHHHHHCCCHHCCCHHHHHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
HAKAMLKMERDGITPDEQGSVGPITQIEEMQKDGIPLAYVGDVVGTGSSRKSATNSVLWF
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCCEEEE
MGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELDVQNMNMGDIIDIFPYEGVV
ECCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCEE
RKNNEEISSFKLSAVLLDEVRAGGRIPLIIGRGLTSRARISLGLEETDLFAKPIDPSASD
ECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCHHHCEECCCCCCCCC
KGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVM
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
QSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSH
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCC
TRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPLYGIK
CEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHH
QGLLTVEKAGKINEFSGRVLEIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQESIEEY
HHHEEHHHCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCHHCCCCCEEEECHHHHHHH
LNSNIVMLKWMIAEGYGDVRTIERRITAMEEWLANPELLSADSDAEYAHVIEIDLADIDQ
HCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEECCCCCC
PILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNIGHFRAAGKMLEEFNGSLSTRLWVAP
CEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
PTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKSTVMSTSTRNFPNRLGT
CCCCCHHHHCCCCCEEEECCCCEEEECCCCCEEECCCCCCCCCHHHEECCCCCCHHHHCC
GANVYLASAELSAVGAILGRIPTKEEYLEYADKINATAADTYRYLNFHKMGAYTEKADTV
CCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHEEHHCCCCCCCCCEE
IFQEPA
EEECCC
>Mature Secondary Structure
MVPRPLDAEQVAALIELVKNPPQGEEEVILDLLENRIPPGVDEAAYVKAGFLTAVTKGEV
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEECCCC
SSPLVSKEKAAELLGTMQGGYNIEPLVSLLDDTELAPIAVKALSHTLLMFDAFYDVEEKA
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHH
KAGNSSAQQVLQSWADAEWFTSKDKVAEKITVKVFKVTGETNTDDLSPAPDAWSRPDIPV
HCCCHHHHHHHHHHCCCHHCCCHHHHHHEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
HAKAMLKMERDGITPDEQGSVGPITQIEEMQKDGIPLAYVGDVVGTGSSRKSATNSVLWF
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCCEEEE
MGEDIPFVPNKRTGGVCLGGKIAPIFYNTMEDSGALPIELDVQNMNMGDIIDIFPYEGVV
ECCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCEEEEEECCCCCCCEEEECCCCCEE
RKNNEEISSFKLSAVLLDEVRAGGRIPLIIGRGLTSRARISLGLEETDLFAKPIDPSASD
ECCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCHHHCEECCCCCCCCC
KGYTLAQKMVGKACGVEGVRAGQYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVM
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
QSFCHTSAYPKPVDVNTHHTLPDFIMNRAGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSH
HHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCC
TRFPLGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPLYGIK
CEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHH
QGLLTVEKAGKINEFSGRVLEIEGVEHLSVEQAFELSDASAERSAAGCTVKLSQESIEEY
HHHEEHHHCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCHHCCCCCEEEECHHHHHHH
LNSNIVMLKWMIAEGYGDVRTIERRITAMEEWLANPELLSADSDAEYAHVIEIDLADIDQ
HCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEEEEECCCCCC
PILCAPNDPDDARLLSDVQGTEIQEVFIGSCMTNIGHFRAAGKMLEEFNGSLSTRLWVAP
CEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
PTKMDKDQLTEEGYYGIFGRAGVRIETPGCSLCMGNQARVADKSTVMSTSTRNFPNRLGT
CCCCCHHHHCCCCCEEEECCCCEEEECCCCCEEECCCCCCCCCHHHEECCCCCCHHHHCC
GANVYLASAELSAVGAILGRIPTKEEYLEYADKINATAADTYRYLNFHKMGAYTEKADTV
CCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHEEHHCCCCCCCCCEE
IFQEPA
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]