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The map label for this gene is fusA

Identifier: 170757683

GI number: 170757683

Start: 3814731

End: 3816803

Strand: Reverse

Name: fusA

Synonym: CLD_0987

Alternate gene names: 170757683

Gene position: 3816803-3814731 (Counterclockwise)

Preceding gene: 170755247

Following gene: 170756880

Centisome position: 96.43

GC content: 30.05

Gene sequence:

>2073_bases
ATGAATTATAGCGCCACCAATCTAAGGAATGTAGGCATAATTGGACATAGTGGATCAGGAAAAACTTCTTTAACAGAAGC
TATTTTATATTTTACAAGCATGGTAGATAGATTAGGTACCATAGAAGAGGGAAATACTGTATCTGATTATGATACAGAGG
AAAAAAAACGTAAAATATCTATATCTACCACAATACAACCGTGTAAATGGAAAGACACAAAAATTAATTTGATTGATATG
CCTGGTTATTTTGATTTTGTAGGAGAAAAGATTCAAGGGTTAAAAGCTGCCGATATTGCTCTTATAGTATTGTCAGCAAC
AGGAAATATACATGTTGAAACAGAAAAATCTTGGAATTATACAACAAAAGAGAAAATACCTAAAGCATTTTATATAAACA
AATTAGATAAAGAAAATATGGATTTTGAAGGCACTTTAAATAAACTTAAAGAAAGATATGGTACATCTGTAGTACCTGTT
CAATACCCTATAGGAAAAGAAGAAAATTTTAAAGGTGTAATTAATGTTATTTCTAATAAAGCAACTATATTTAATGATAA
AACACATGAGATAGAAATAAAAGAAGTACCAAATGAATTATTAGATGAAGTTAATGAATGTAAAAATATGATTGTAGAAG
CAGTAGCTGAAACAGATGAGAAGCTTTTAGAAAAATATTTTGATGAAGGTACACTAAGTGAAAAGGATATATATGAAGGA
TTAATGGCAGGAACTGTAAAAGGAGAAATAGTTCCGGTAATGTGTGGGTCAGCCATAAAGGCTATAGGTATTGAAACCTT
TTTAGAGGATGTAGTTCAATGGTTTCCATCACCAGCAGAAACAAAAAAAATGGAAGTATCAGATGGTATAAATAAAAATA
ATATAGAAATAAATTGTAATGAAAAAGAACCATTTTCAGCACTGGTTTTTAAAACTATTATAGATCCTTTTATAGGTAGA
TTATCATTATTCAAAGTAACGAGTGGAGTGCTAAATGAAGAGAGCTCTATTTATAATTCAACAAAAGATAAAAAGGAGAA
GGTAGGTAAATTATATTTCCTAAGGGGCAAGCAACAAATTCAAACAGATAGAGTAATTTCAGGAGATATAGGGGCAGTTA
GTAAATTACAATATACTTTTACAGGAGATACTTTGTGTGATTTAAAAAGAAAAATATTATTGGAAAATATAGATTTTCCT
AAAGCTAATATGACTATGGCAGCATTACCTAAATCAAAAAATGATGAAGATAAAATGTTTTATGCCCTAGACAAATTGGT
GGAGGAAGACCCTACGATATATATAAGTAGAGATTTAGAAAATGCAGAAACATTAATATCAGGTATGGGAGATATACATT
TAGAAGTAATAGCAAATAGAGTGAAAAATAAATTTGGGACAGAAATGGTATTGGATTCACCTAAAATTCCATATAGGGAG
ACTATAAAATCTATATCTAAGGTTCAAGGAAAGCATAAAAAGCAATCTGGCGGACATGGTCAATATGGAGATGTATTTAT
AAAATTCGAACCAAATAAAAATAGCGAGTTTGAATTTGTGAATAATATAGTTGGAGGGGTAGTTCCTAAACAATATATTC
CTGCAGTAGAAGAAGGAATTTTAGAATGCATGAAGAAGGGAGTCTTAGCAGAATATCCTGTTATAGGAATTAAGGCTACA
ATATACGATGGTTCATATCATTCAGTAGATTCATCTGAAATGGCTTTTAAAATTGCAGCATCTATTGCATTTAAAAAAGG
AATAAAGGAAGCAAAGCCAACTTTATTAGAACCTATAATGCGTTTAGAAGTGATATCATCAGAGGAATATATGGGGGATA
TTATAGGAGATATAAATAGAAAGAGAGGTAAAATTCTAGGCATGGAGCAGGAAGCTAATAATTTAGAAAGAATTGTAGCA
GAAGTACCTCAGGCGGAAATCCTAAATTATTCTACAGATTTAAGGAGTATAACTCAAGGTATGGGAGAATTTTCTTTAAG
CTTTGAAAGATATGATGAAATTTTAGATCCTATTGAGGTTGAAAAAGTATTATCTAGTAAAAATAAAATATAG

Upstream 100 bases:

>100_bases
GTATAGTCTGATTATTAAGAAAATTAGTAATTTTTGAAAGGATTTTATATTATCAAATAGAATATATATTTAAGCCATAA
AATAGATAGGGGGAATTAGT

Downstream 100 bases:

>100_bases
TATAAAAATCACCTAGTTTCTAGGTGATTTTTATACTATATTTTATTTAGTGTTTTTTATTATTTTAGTATTTCCATTTA
GATCAAATACCTTTTGAAAT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MNYSATNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVETEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIGKEENFKGVINVISNKATIFNDKTHEIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDVVQWFPSPAETKKMEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEMVLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLAEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI

Sequences:

>Translated_690_residues
MNYSATNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVETEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIGKEENFKGVINVISNKATIFNDKTHEIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDVVQWFPSPAETKKMEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEMVLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLAEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI
>Mature_690_residues
MNYSATNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKISISTTIQPCKWKDTKINLIDM
PGYFDFVGEKIQGLKAADIALIVLSATGNIHVETEKSWNYTTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPV
QYPIGKEENFKGVINVISNKATIFNDKTHEIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDVVQWFPSPAETKKMEVSDGINKNNIEINCNEKEPFSALVFKTIIDPFIGR
LSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQIQTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFP
KANMTMAALPKSKNDEDKMFYALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEMVLDSPKIPYRE
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGILECMKKGVLAEYPVIGIKAT
IYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIMRLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVA
EVPQAEILNYSTDLRSITQGMGEFSLSFERYDEILDPIEVEKVLSSKNKI

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=682, Percent_Identity=33.2844574780059, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI19923640, Length=734, Percent_Identity=27.2479564032698, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI25306283, Length=399, Percent_Identity=29.0726817042606, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI25306287, Length=294, Percent_Identity=30.952380952381, Blast_Score=158, Evalue=2e-38,
Organism=Escherichia coli, GI1789738, Length=680, Percent_Identity=40, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI1790835, Length=494, Percent_Identity=24.6963562753036, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI48994988, Length=134, Percent_Identity=32.8358208955224, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=677, Percent_Identity=29.9852289512555, Blast_Score=350, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17556745, Length=708, Percent_Identity=24.1525423728814, Blast_Score=215, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6323098, Length=677, Percent_Identity=31.7577548005908, Blast_Score=351, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6322359, Length=773, Percent_Identity=27.1668822768435, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6324707, Length=825, Percent_Identity=21.4545454545455, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=825, Percent_Identity=21.4545454545455, Blast_Score=111, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24582462, Length=684, Percent_Identity=33.3333333333333, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI221458488, Length=711, Percent_Identity=27.7074542897328, Blast_Score=253, Evalue=4e-67,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77340; Mature: 77340

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSATNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKIS
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEE
ISTTIQPCKWKDTKINLIDMPGYFDFVGEKIQGLKAADIALIVLSATGNIHVETEKSWNY
EEECCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCEEEEEEECCCCEEEEECCCCCC
TTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPVQYPIGKEENFKGVINVISNK
CHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCC
ATIFNDKTHEIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
EEEECCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDVVQWFPSPAETKKMEVSDGINKNNIEINCN
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHCCCCCCCEEEECC
EKEPFSALVFKTIIDPFIGRLSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCEEEECCCHHH
QTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFPKANMTMAALPKSKNDEDKMF
HHCCEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHH
YALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEMVLDSPKIPYRE
HHHHHHHCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHH
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGI
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCHHHHHH
LECMKKGVLAEYPVIGIKATIYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIM
HHHHHCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVAEVPQAEILNYSTDLRSITQG
HHHHHCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHC
MGEFSLSFERYDEILDPIEVEKVLSSKNKI
HHHHEECHHHHHHHHCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNYSATNLRNVGIIGHSGSGKTSLTEAILYFTSMVDRLGTIEEGNTVSDYDTEEKKRKIS
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEE
ISTTIQPCKWKDTKINLIDMPGYFDFVGEKIQGLKAADIALIVLSATGNIHVETEKSWNY
EEECCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEECCEEEEEEECCCCEEEEECCCCCC
TTKEKIPKAFYINKLDKENMDFEGTLNKLKERYGTSVVPVQYPIGKEENFKGVINVISNK
CHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCC
ATIFNDKTHEIEIKEVPNELLDEVNECKNMIVEAVAETDEKLLEKYFDEGTLSEKDIYEG
EEEECCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LMAGTVKGEIVPVMCGSAIKAIGIETFLEDVVQWFPSPAETKKMEVSDGINKNNIEINCN
HHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHCCCCCCCEEEECC
EKEPFSALVFKTIIDPFIGRLSLFKVTSGVLNEESSIYNSTKDKKEKVGKLYFLRGKQQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCEEEECCCHHH
QTDRVISGDIGAVSKLQYTFTGDTLCDLKRKILLENIDFPKANMTMAALPKSKNDEDKMF
HHCCEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHH
YALDKLVEEDPTIYISRDLENAETLISGMGDIHLEVIANRVKNKFGTEMVLDSPKIPYRE
HHHHHHHCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHH
TIKSISKVQGKHKKQSGGHGQYGDVFIKFEPNKNSEFEFVNNIVGGVVPKQYIPAVEEGI
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHCCHHHHHH
LECMKKGVLAEYPVIGIKATIYDGSYHSVDSSEMAFKIAASIAFKKGIKEAKPTLLEPIM
HHHHHCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLEVISSEEYMGDIIGDINRKRGKILGMEQEANNLERIVAEVPQAEILNYSTDLRSITQG
HHHHHCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHC
MGEFSLSFERYDEILDPIEVEKVLSSKNKI
HHHHEECHHHHHHHHCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA