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The map label for this gene is murD

Identifier: 170755247

GI number: 170755247

Start: 3827817

End: 3829193

Strand: Reverse

Name: murD

Synonym: CLD_0974

Alternate gene names: 170755247

Gene position: 3829193-3827817 (Counterclockwise)

Preceding gene: 170754288

Following gene: 170757683

Centisome position: 96.74

GC content: 28.61

Gene sequence:

>1377_bases
ATGAAAAGCAATTTTTCTAAGTTTAAGGATTTTATAAAGTATAAAAAAGTAGCAGTGGTAGGTATAGGGGTAAGCAATAG
ACCTTTAATAAAATTTTTAGTTAAGTTAGGTGCAAAAGTAACTGCTTTTGACAAAAAACATAGAGAAAAATTAGGAAGTA
TAAGTTTTGAATTAGAAGAGATAGGTGTAGATTTAGTATTAGGTGAAAATTATTTAGATAAATTAGATGGATATGATGTT
ATTTTTAAAACTCCATCTATGAGAATAGATAGACCGGAATTTGTTAAGGCAAAAGAATCTGGAGCTTATATTACATCAGA
AATGGAAGAGTTTATAAAATATTGTCCAGCTAAAGTTTTTGGTATAACAGGAAGTGATGGTAAAACAACTACTACAACAT
TAGTGTATGAAATGTTAAAAAGAGAAGGTTATAGAACTTGGGTAGGGGGGAATATAGGAACACCTTTATTTGCTAATATT
GAAGAAATGAAGGAAGACCATATGGTTGTATTAGAACTATCAAGTTTTCAGCTTATGACAATGGATGTATCACCAGAGAT
TTCTTTGATCACTAATTTAAGTCCTAATCATTTAGATGTGCATAAAGATTTTGAGGAATATGTATGGGCTAAAAAAAATA
TATTTAAGTATCAATCAAGTAATAACTTGTTAGTATTAAATAAAGATGATGATTTAACAAATGGGATGGAGAATGAAGCT
TTAGGAGATGTTTTAAAGTTTAGTCTTGTAGAAAAAGTATATAATGGGGCTTGCTTATCAAATAATAAGCTTACTATACA
AGGAAAAGAAGTATGTGATTCTAAAGATATAAATCTTAAAGGTAGGCATAATATAGCTAATTTATTAGCAGCTTTTTGCA
TGGTAAATAAATATGTATCAATAGATAGTATGAAATATGTAGCTACAAATTTTTCAGGTGTAGAACATAGATGCGAATTT
ATAAGAGAAGTTAATGGGGTTAAATACTATAATGATTCAATAGCATCAAGCCCTAGTAGAACTTTAGCAGGACTAAATTC
TTTTGAAAAACCAGTAATATTAATAGCTGGTGGATATGATAAAAAGATACCTTTTGAACCATTAGCAGAAGGTGGATATG
ATAAAATTAAAATATTGATATTAATGGGAGATACGAAGAATAAAATAAGATCAGCTTTTGAAAAAGTAATCTCCTATAAA
AAGTGTGAGATGGAAATAGTTATAGTAAATAGTATGGAGGAAGCTGTGAAAGTAGCTGATGATATGGCGGAAAAAGGAGA
TATAATAACTTTATCACCAGCTTGTGCTAGCTTTGATATGTATCCTAACTTTGAAATAAGGGGAAATGAATTTAAGAATA
TAGTAAATAGTTTATAA

Upstream 100 bases:

>100_bases
TAATTACAATATAAAAATATAAAAGTATTTAAAATGTTTTATAAATAAATTAAAGTGATATAATATTAGGTGGATTAATA
CTTAGTTTGGAGGTTTATAA

Downstream 100 bases:

>100_bases
TTTTGTATATTAGATTTATAGAGTTATATTTAAAATTTTTAGAATATAATGTATGTGTAATTCCCTAATTAAAAGTAGTT
TAATATTAAAAGTTCTGCTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISFELEEIGVDLVLGENYLDKLDGYDV
IFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVFGITGSDGKTTTTTLVYEMLKREGYRTWVGGNIGTPLFANI
EEMKEDHMVVLELSSFQLMTMDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDINLKGRHNIANLLAAFCMVNKYVSIDSMKYVATNFSGVEHRCEF
IREVNGVKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYDKKIPFEPLAEGGYDKIKILILMGDTKNKIRSAFEKVISYK
KCEMEIVIVNSMEEAVKVADDMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL

Sequences:

>Translated_458_residues
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISFELEEIGVDLVLGENYLDKLDGYDV
IFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVFGITGSDGKTTTTTLVYEMLKREGYRTWVGGNIGTPLFANI
EEMKEDHMVVLELSSFQLMTMDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDINLKGRHNIANLLAAFCMVNKYVSIDSMKYVATNFSGVEHRCEF
IREVNGVKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYDKKIPFEPLAEGGYDKIKILILMGDTKNKIRSAFEKVISYK
KCEMEIVIVNSMEEAVKVADDMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL
>Mature_458_residues
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISFELEEIGVDLVLGENYLDKLDGYDV
IFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVFGITGSDGKTTTTTLVYEMLKREGYRTWVGGNIGTPLFANI
EEMKEDHMVVLELSSFQLMTMDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDINLKGRHNIANLLAAFCMVNKYVSIDSMKYVATNFSGVEHRCEF
IREVNGVKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYDKKIPFEPLAEGGYDKIKILILMGDTKNKIRSAFEKVISYK
KCEMEIVIVNSMEEAVKVADDMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=30.1969365426696, Blast_Score=168, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 51527; Mature: 51527

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISFELEE
CCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHCCCEEEHHH
IGVDLVLGENYLDKLDGYDVIFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVF
CCEEEEECCHHHHHCCCCEEEEECCCCCCCCHHHHEECCCCCCHHHHHHHHHHHCCCEEE
GITGSDGKTTTTTLVYEMLKREGYRTWVGGNIGTPLFANIEEMKEDHMVVLELSSFQLMT
EEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCEEEEEECCEEEEE
MDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
EECCCCEEEEECCCCCCCCHHCCHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCCHHH
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDINLKGRHNIANLLAAFCMVNKYVS
HHHHHHHHHHHHHHCCCEECCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
IDSMKYVATNFSGVEHRCEFIREVNGVKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYD
CCCEEEECCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHCCCCCCCEEEEECCCC
KKIPFEPLAEGGYDKIKILILMGDTKNKIRSAFEKVISYKKCEMEIVIVNSMEEAVKVAD
CCCCCCHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHH
DMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL
HHHHCCCEEEECCCCCCCCCCCCEEECCHHHHHHHHCC
>Mature Secondary Structure
MKSNFSKFKDFIKYKKVAVVGIGVSNRPLIKFLVKLGAKVTAFDKKHREKLGSISFELEE
CCCCHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCEEEEHHHHHHHHHCCCEEEHHH
IGVDLVLGENYLDKLDGYDVIFKTPSMRIDRPEFVKAKESGAYITSEMEEFIKYCPAKVF
CCEEEEECCHHHHHCCCCEEEEECCCCCCCCHHHHEECCCCCCHHHHHHHHHHHCCCEEE
GITGSDGKTTTTTLVYEMLKREGYRTWVGGNIGTPLFANIEEMKEDHMVVLELSSFQLMT
EEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHCCCCEEEEEECCEEEEE
MDVSPEISLITNLSPNHLDVHKDFEEYVWAKKNIFKYQSSNNLLVLNKDDDLTNGMENEA
EECCCCEEEEECCCCCCCCHHCCHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCCHHH
LGDVLKFSLVEKVYNGACLSNNKLTIQGKEVCDSKDINLKGRHNIANLLAAFCMVNKYVS
HHHHHHHHHHHHHHCCCEECCCEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
IDSMKYVATNFSGVEHRCEFIREVNGVKYYNDSIASSPSRTLAGLNSFEKPVILIAGGYD
CCCEEEECCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCHHHHHCCCCCCCEEEEECCCC
KKIPFEPLAEGGYDKIKILILMGDTKNKIRSAFEKVISYKKCEMEIVIVNSMEEAVKVAD
CCCCCCHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHH
DMAEKGDIITLSPACASFDMYPNFEIRGNEFKNIVNSL
HHHHCCCEEEECCCCCCCCCCCCEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA