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The map label for this gene is purA

Identifier: 170757650

GI number: 170757650

Start: 3928548

End: 3929834

Strand: Reverse

Name: purA

Synonym: CLD_0858

Alternate gene names: 170757650

Gene position: 3929834-3928548 (Counterclockwise)

Preceding gene: 170754343

Following gene: 170756592

Centisome position: 99.28

GC content: 32.01

Gene sequence:

>1287_bases
ATGTCAGCTTTTATAGTATTAGGGGCCCAATGGGGCGATGAAGGTAAAGGAAAAATGACTGATTATTTAGCTGAAAATGC
AGATGTAGTTGTAAGATTTCAAGGTGGTAATAACGCAGGTCATACAGTAGTTGTAGGAGAAAAGGAATATAAATTACACT
TAATACCTTCAGGTATACTGTATAATGACAAACTAAATGTAATAGGAAATGGAGTAGTTTTAGATCCAAAGGCTTTATTT
GAAGAAATAAACTATTTAGAATCTTTAGGAGTAGAAATAACACCAGATAGATTAATAATAAGTGATAGAGCTCATGTAAT
AATGCCATATCATAGGGTATTAGATGGAATAAAAGAAAGAGCTAGAGGAAATAAAGATATAGGAACTACAGGAAAGGGTA
TAGGACCAAGTTATACGGATAAGATGGAAAGAAGCGGTATAAGGGTTTGCGATCTTATACATAAAGAAGTTTTTGAAGAA
AATTTGAAAGAAACTTTAGAAGTAAAAAATAAAATAATAACAGAAGTATTTGGTGGAGAGGCTTTAGACTATAATGAAAT
CTATAATGAATATTTAGGATATGCAGAAAAATTAAGACCTTTTGTAAAAGATATATCTGTTATAGTTAACAAAAAAATAA
AAGATGGTAAAGAAGTGTTATTTGAAGGAGCTCAAGGAACATTACTTGATATAGATTATGGAACATATCCTTATGTAACA
TCTTCAAGCACAATAGCTGGAGGAGTTTGCATAGGAGCAGGTGTAGGGCCAACAGCTATAACTAATGCAGTAGGTATAGC
AAAAGCATATACAACAAGAGTAGGAAAAGGTCCTTTCCCAACAGAGCTTTTAGATAGCACTGGAGACTGGGTAAGAGAAA
AGGGACATGAGTTTGGAGTTACTACAGGGAGAGCAAGAAGATGTGGATGGTTAGATTTAGTTATATTAAAAACTTCAGCG
AGAATATCAGGACTTACAAGTTTTGCAGTAACAAAAATTGATACATTAGCAGGATTAGATACATTAAAAGTATGCACAGG
ATATAGACTAAATGGAGAAATTATAGATTATGTTCCTGCTAGCTTAGAAGATTTAGCAAAATGTGAACCAATATATGAAG
AATTTGAAGGTTGGGATGATAGTATAGCTAATGCTAGATGCTATGAAGATTTACCTGAAAATGCTATAAAATATTTAAAG
AAAATAGAAGATTTCACAGAAACAAAGGTATCTATAGTTTCTGTAGGTCCAAAAAGAGATCAAACTATGATGATATCAGA
AATTTAA

Upstream 100 bases:

>100_bases
TATATATTGTAGAAGTATTTTATTTAAAACTAAAATTACATAGCATATAAATCATATTTAAATATAATAATTTTCTTAAA
ATGAAAGTGAGGAAAATAAT

Downstream 100 bases:

>100_bases
TGTATATTATAGGGCATAGTAATAAAGAATATTAATATATATAAAAAATAAATATAATATCTTTAAAGAATACTAATTTA
TTGATATAATAATAATTAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGILYNDKLNVIGNGVVLDPKALF
EEINYLESLGVEITPDRLIISDRAHVIMPYHRVLDGIKERARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEE
NLKETLEVKNKIITEVFGGEALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVT
SSSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGVTTGRARRCGWLDLVILKTSA
RISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPASLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLK
KIEDFTETKVSIVSVGPKRDQTMMISEI

Sequences:

>Translated_428_residues
MSAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGILYNDKLNVIGNGVVLDPKALF
EEINYLESLGVEITPDRLIISDRAHVIMPYHRVLDGIKERARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEE
NLKETLEVKNKIITEVFGGEALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVT
SSSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGVTTGRARRCGWLDLVILKTSA
RISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPASLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLK
KIEDFTETKVSIVSVGPKRDQTMMISEI
>Mature_427_residues
SAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGILYNDKLNVIGNGVVLDPKALFE
EINYLESLGVEITPDRLIISDRAHVIMPYHRVLDGIKERARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEEN
LKETLEVKNKIITEVFGGEALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVTS
SSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGVTTGRARRCGWLDLVILKTSAR
ISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPASLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLKK
IEDFTETKVSIVSVGPKRDQTMMISEI

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=46.135831381733, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=45.5399061032864, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=43.8287153652393, Blast_Score=332, Evalue=4e-91,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=48.1132075471698, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=45.9207459207459, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=45.9207459207459, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=46.0829493087558, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=43.9716312056738, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47073; Mature: 46942

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGIL
CCEEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCEE
YNDKLNVIGNGVVLDPKALFEEINYLESLGVEITPDRLIISDRAHVIMPYHRVLDGIKER
ECCCEEEEECCEEECHHHHHHHHHHHHHCCCEECCCEEEEECCCCEEECHHHHHHHHHHH
ARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEENLKETLEVKNKIITEVFGGE
HCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCCEEE
SSSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGV
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHCCCCCCC
TTGRARRCGWLDLVILKTSARISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPA
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCEECCCEEECCCC
SLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLKKIEDFTETKVSIVSVGPKRD
CHHHHHHCCHHHHHHCCCCHHHCCCHHHHHCHHHHHHHHHHHHHHHHCEEEEEEECCCCC
QTMMISEI
CEEEEECC
>Mature Secondary Structure 
SAFIVLGAQWGDEGKGKMTDYLAENADVVVRFQGGNNAGHTVVVGEKEYKLHLIPSGIL
CEEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCEE
YNDKLNVIGNGVVLDPKALFEEINYLESLGVEITPDRLIISDRAHVIMPYHRVLDGIKER
ECCCEEEEECCEEECHHHHHHHHHHHHHCCCEECCCEEEEECCCCEEECHHHHHHHHHHH
ARGNKDIGTTGKGIGPSYTDKMERSGIRVCDLIHKEVFEENLKETLEVKNKIITEVFGGE
HCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALDYNEIYNEYLGYAEKLRPFVKDISVIVNKKIKDGKEVLFEGAQGTLLDIDYGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCCEEE
SSSTIAGGVCIGAGVGPTAITNAVGIAKAYTTRVGKGPFPTELLDSTGDWVREKGHEFGV
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHCCCCCCC
TTGRARRCGWLDLVILKTSARISGLTSFAVTKIDTLAGLDTLKVCTGYRLNGEIIDYVPA
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCEECCCEEECCCC
SLEDLAKCEPIYEEFEGWDDSIANARCYEDLPENAIKYLKKIEDFTETKVSIVSVGPKRD
CHHHHHHCCHHHHHHCCCCHHHCCCHHHHHCHHHHHHHHHHHHHHHHCEEEEEEECCCCC
QTMMISEI
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA