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The map label for this gene is wcaL [C]

Identifier: 170757348

GI number: 170757348

Start: 3346656

End: 3347750

Strand: Reverse

Name: wcaL [C]

Synonym: CLD_1439

Alternate gene names: 170757348

Gene position: 3347750-3346656 (Counterclockwise)

Preceding gene: 170756338

Following gene: 170754425

Centisome position: 84.58

GC content: 28.4

Gene sequence:

>1095_bases
ATGAAAATATGTTATTTAGCTGATGCTAATAGTACACATACTAAAAAATGGTGTAGATTTTTTAAGAATAAAGGATATGA
TATACATGTAATATCATTAAATGATGGAGATATAGACGGAGTTACTGTACATAGTTTTAACAAAGATTTAGATAAGATAA
GAAAAGGAAGCTCTTTTAATAAAATTTCTTATATAAGATATTTTAGTGACATAAAAAAAATTATAGAAAAAATAAAACCG
GATGTTGTTCATGCGCATTATGCTACTAGTTATGGTTTATTAGGAGCATTATCAGGATTTCATCCTTATATAATATCTGT
TTGGGGTAGTGATGTATATGATTTTCCTAAGGGAAGCTATGTAAAAAGAAAAATGGTTGAGTATAATTTGTCCAAAGCAG
ATATTATAATGTCTACCAGTAAAGTAATGGCAAAAGAAACAAATCAGTATACTACTAAAAATATAGAAATAACCCCTTTT
GGAGTAAATATAGATACATTTAAGCCTTACGCTGGTAAATATGAAAAAAAAGAAAATTTAGTAATAGGTACAGTTAAAAC
GTTAGAGCCTAAATATGGTATAGAGTACTTAGTTAGAGCTTTTGCAAAGGTAAAGCAAAAACATAGTAATATTAAGTTAG
AAATTGCAGGGGTAGGGGACCAAAAGAACTTTTTACTTAATTTATGTAATGAACTTAATATAAAAGACCATGTTAAATTT
TTAGGGTTTATAAATCAAGAAAAGGTAATAGAGGCCTTTAATAGATTTGATGTAGCTGTATTTCCTTCCACATTAGATAG
TGAAAGTTTCGGGGTAGCGGCAGTAGAAGCACAGGCATGTGGAACTCCAGTAATTGTTTCTAATGTAGGAGGATTACCTG
AAGCTACATCTCCTAATAATAGTAGTCTATTAGTAAATAAAAAAAGTGTAGATGAGTTAGCAGAGGCTATAGAAAGATTA
ATTGAGGATGACAATTTAAGGATAAATATGGGTAAGACAGGAAGAAAGTTTGTGGAAGATAATTTTAATATAGAAGATAA
TTTTAATAATGTAGATATAATATACAAATCTATAATAGATGGAGGAGATAATTAA

Upstream 100 bases:

>100_bases
ATAGATAATATTATTAATAAACATACATGGAAAGCTAGAGCTATAAAAGTAGTAGAGGATTTAACAAATCTTCAATAATA
CTTTTAATGGAGGAATAATA

Downstream 100 bases:

>100_bases
TGAAGATATTAACAGTAGTTGGTGCACGTCCACAATTTATAAAAGCAGCAGCAGTTTCAAATATAATAAGAAAAGAGCAT
GAAGAAATATTAGTTCATAC

Product: glycosyl transferase, group 1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKICYLADANSTHTKKWCRFFKNKGYDIHVISLNDGDIDGVTVHSFNKDLDKIRKGSSFNKISYIRYFSDIKKIIEKIKP
DVVHAHYATSYGLLGALSGFHPYIISVWGSDVYDFPKGSYVKRKMVEYNLSKADIIMSTSKVMAKETNQYTTKNIEITPF
GVNIDTFKPYAGKYEKKENLVIGTVKTLEPKYGIEYLVRAFAKVKQKHSNIKLEIAGVGDQKNFLLNLCNELNIKDHVKF
LGFINQEKVIEAFNRFDVAVFPSTLDSESFGVAAVEAQACGTPVIVSNVGGLPEATSPNNSSLLVNKKSVDELAEAIERL
IEDDNLRINMGKTGRKFVEDNFNIEDNFNNVDIIYKSIIDGGDN

Sequences:

>Translated_364_residues
MKICYLADANSTHTKKWCRFFKNKGYDIHVISLNDGDIDGVTVHSFNKDLDKIRKGSSFNKISYIRYFSDIKKIIEKIKP
DVVHAHYATSYGLLGALSGFHPYIISVWGSDVYDFPKGSYVKRKMVEYNLSKADIIMSTSKVMAKETNQYTTKNIEITPF
GVNIDTFKPYAGKYEKKENLVIGTVKTLEPKYGIEYLVRAFAKVKQKHSNIKLEIAGVGDQKNFLLNLCNELNIKDHVKF
LGFINQEKVIEAFNRFDVAVFPSTLDSESFGVAAVEAQACGTPVIVSNVGGLPEATSPNNSSLLVNKKSVDELAEAIERL
IEDDNLRINMGKTGRKFVEDNFNIEDNFNNVDIIYKSIIDGGDN
>Mature_364_residues
MKICYLADANSTHTKKWCRFFKNKGYDIHVISLNDGDIDGVTVHSFNKDLDKIRKGSSFNKISYIRYFSDIKKIIEKIKP
DVVHAHYATSYGLLGALSGFHPYIISVWGSDVYDFPKGSYVKRKMVEYNLSKADIIMSTSKVMAKETNQYTTKNIEITPF
GVNIDTFKPYAGKYEKKENLVIGTVKTLEPKYGIEYLVRAFAKVKQKHSNIKLEIAGVGDQKNFLLNLCNELNIKDHVKF
LGFINQEKVIEAFNRFDVAVFPSTLDSESFGVAAVEAQACGTPVIVSNVGGLPEATSPNNSSLLVNKKSVDELAEAIERL
IEDDNLRINMGKTGRKFVEDNFNIEDNFNNVDIIYKSIIDGGDN

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=271, Percent_Identity=23.2472324723247, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788357, Length=320, Percent_Identity=22.8125, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI9755344, Length=347, Percent_Identity=22.1902017291066, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 40935; Mature: 40935

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKICYLADANSTHTKKWCRFFKNKGYDIHVISLNDGDIDGVTVHSFNKDLDKIRKGSSFN
CEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCHHHHHHHHCCCCCC
KISYIRYFSDIKKIIEKIKPDVVHAHYATSYGLLGALSGFHPYIISVWGSDVYDFPKGSY
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCH
VKRKMVEYNLSKADIIMSTSKVMAKETNQYTTKNIEITPFGVNIDTFKPYAGKYEKKENL
HHHHHHHCCCCCHHEEEEHHHHHHHHCCCEEECEEEEEEECCCCCCCCCCCCCCCCCCCE
VIGTVKTLEPKYGIEYLVRAFAKVKQKHSNIKLEIAGVGDQKNFLLNLCNELNIKDHVKF
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHH
LGFINQEKVIEAFNRFDVAVFPSTLDSESFGVAAVEAQACGTPVIVSNVGGLPEATSPNN
HHCCCHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEHHHCCCCEEEECCCCCCCCCCCCC
SSLLVNKKSVDELAEAIERLIEDDNLRINMGKTGRKFVEDNFNIEDNFNNVDIIYKSIID
CEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCCCCCHHHHEEHHHC
GGDN
CCCC
>Mature Secondary Structure
MKICYLADANSTHTKKWCRFFKNKGYDIHVISLNDGDIDGVTVHSFNKDLDKIRKGSSFN
CEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCHHHHHHHHCCCCCC
KISYIRYFSDIKKIIEKIKPDVVHAHYATSYGLLGALSGFHPYIISVWGSDVYDFPKGSY
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCH
VKRKMVEYNLSKADIIMSTSKVMAKETNQYTTKNIEITPFGVNIDTFKPYAGKYEKKENL
HHHHHHHCCCCCHHEEEEHHHHHHHHCCCEEECEEEEEEECCCCCCCCCCCCCCCCCCCE
VIGTVKTLEPKYGIEYLVRAFAKVKQKHSNIKLEIAGVGDQKNFLLNLCNELNIKDHVKF
EEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHH
LGFINQEKVIEAFNRFDVAVFPSTLDSESFGVAAVEAQACGTPVIVSNVGGLPEATSPNN
HHCCCHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEHHHCCCCEEEECCCCCCCCCCCCC
SSLLVNKKSVDELAEAIERLIEDDNLRINMGKTGRKFVEDNFNIEDNFNNVDIIYKSIID
CEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCCCCCHHHHEEHHHC
GGDN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]